Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,681,333	T→C	E203G (GAA→GGA)	yhjJ ←	peptidase M16 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,681,333	0	T	C	100.0%	143.4 / NA	41	E203G (GAA→GGA)	yhjJ	peptidase M16 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/21);  total (20/21)

GTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTTCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGT  >  NC_000913/3681198‑3681468                                                                                                                                        |                                                                                                                                        gTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGc                                                                                                                                     >  2:857886/1‑140 (MQ=255)    tCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTc                                                                                                                                  >  1:894121/1‑140 (MQ=255)        gTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACg                                                                                                                              <  1:190963/140‑1 (MQ=255)         tttGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACgg                                                                                                                             >  1:327728/1‑140 (MQ=255)          ttGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACggg                                                                                                                            <  2:936164/140‑1 (MQ=255)                      tCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCgg                                                                                                                <  1:316894/140‑1 (MQ=255)                       cGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGa                                                                                                               >  2:127776/1‑140 (MQ=255)                        gCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGAt                                                                                                              <  1:857886/140‑1 (MQ=255)                           aaaCGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCgg                                                                                                           <  2:121947/140‑1 (MQ=255)                               gTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAgg                                                                                                       >  2:632769/1‑140 (MQ=255)                                  ttGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATc                                                                                                    <  1:330166/140‑1 (MQ=255)                                  ttGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATc                                                                                                    <  1:518428/140‑1 (MQ=255)                                      ttATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGa                                                                                                <  1:477292/140‑1 (MQ=255)                                      ttATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGa                                                                                                <  1:304045/140‑1 (MQ=255)                                            ggTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAAc                                                                                          <  1:822245/140‑1 (MQ=255)                                                     ccGAGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGAc                                                                                 <  2:327728/140‑1 (MQ=255)                                                        aGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCt                                                                              >  2:650691/1‑140 (MQ=255)                                                               aTCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGa                                                                                  >  1:237159/1‑129 (MQ=255)                                                               aTCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGa                                                                                  >  1:617708/1‑129 (MQ=255)                                                               aTCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGa                                                                                  <  2:617708/129‑1 (MQ=255)                                                               aTCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGa                                                                                  <  2:237159/129‑1 (MQ=255)                                                               aTCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGAc                                                                       >  1:982013/1‑140 (MQ=255)                                                                         cccACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGcca                                                             >  2:283014/1‑140 (MQ=255)                                                                          ccaccaccaGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGccac                                                            <  1:283014/140‑1 (MQ=255)                                                                               accaGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGcc                                                       <  1:814138/140‑1 (MQ=255)                                                                               accaGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGcc                                                       <  1:650691/140‑1 (MQ=255)                                                                               accaGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAAACGATAGCGCCACCAGcc                                                       <  1:127776/140‑1 (MQ=255)                                                                                   gTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCctct                                                   >  2:758258/1‑140 (MQ=255)                                                                                       cGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAg                                               >  2:78211/1‑140 (MQ=255)                                                                                                 tCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGcc                                                       >  1:43088/1‑122 (MQ=255)                                                                                                 tCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGcc                                                       <  1:818336/122‑1 (MQ=255)                                                                                                 tCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGcc                                                       >  2:818336/1‑122 (MQ=255)                                                                                                 tCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGcc                                                       <  2:43088/122‑1 (MQ=255)                                                                                                 tCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAgg                                     >  2:66715/1‑140 (MQ=255)                                                                                                  ccGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGc                                    <  2:349781/140‑1 (MQ=255)                                                                                                      ggTGTACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGATGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAgg                                >  1:649885/1‑140 (MQ=255)                                                                                                         gtACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTg                             >  1:150501/1‑140 (MQ=255)                                                                                                          tACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCa                                  <  2:687896/134‑1 (MQ=255)                                                                                                          tACCATTTCTGATAGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCa                                  >  1:687896/1‑134 (MQ=255)                                                                                                                       aGAAATCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGa               >  2:323348/1‑140 (MQ=255)                                                                                                                         aaaTCTTTAATTTTTCCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACt             >  1:435484/1‑140 (MQ=255)                                                                                                                                   tttttcCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGcgcg   <  2:13021/140‑1 (MQ=255)                                                                                                                                    ttttcCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGt  <  1:323348/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        GTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAGCGTCATTGCATCCGGGGTGTACCATTTCTGATAGAAATCTTTAATTTTTTCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGT  >  NC_000913/3681198‑3681468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑