Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,740,106	A→G	A134A (GCA→GCG)	avtA →	valine‑‑pyruvate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,740,106	0	A	G	100.0%	189.4 / NA	54	A134A (GCA→GCG)	avtA	valine‑‑pyruvate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/31);  total (23/31)

AGAAGTTGGGTTGGGATATCGAACCACAGAATATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCACCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAGGCCAGTTTAAATACCACGTCGATTTTGAGCATCTGCATA  >  NC_000913/3739970‑3740230                                                                                                                                         |                                                                                                                             agaAGTTGGGTTGGGATATCGAACCACAGAATATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTAATTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCg                                                                                                                           <  2:282930/140‑1 (MQ=255)  gaAGTTGGGTTGGGATATCGAACCACAGAATATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCCGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCgg                                                                                                                          <  2:175670/140‑1 (MQ=255)   aaGTTGGGTTGGGATATCGAACCACAGAATATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGa                                                                                                                         <  2:510995/140‑1 (MQ=255)     gttgggttggGATATCGAACCACAGAATATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGa                                                                                                                         >  2:579418/1‑138 (MQ=255)     gttgggttggGATATCGAACCACAGAATATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGa                                                                                                                         <  1:579418/138‑1 (MQ=255)       tgggttgggATATCGAACCACAGAATATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATAc                                                                                                                     <  1:889769/140‑1 (MQ=255)              ggATATCGAACCACAGAATATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCt                                                                                                              <  1:821603/140‑1 (MQ=255)                   tCGAACCACAGAATATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCt                                                                                                         >  1:521231/1‑140 (MQ=255)                   tCGAACCACAGAATATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGCGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCt                                                                                                         >  1:33705/1‑140 (MQ=255)                    cGAACCACAGAATATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTg                                                                                                        >  1:983275/1‑140 (MQ=255)                        ccACAGAATATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGc                                                                                                    >  2:128695/1‑140 (MQ=255)                         cacaGAATATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGcc                                                                                                   >  2:281832/1‑140 (MQ=255)                            aGAATATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGa                                                                                                <  1:347698/140‑1 (MQ=255)                             gAATATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGAc                                                                                               <  1:281832/140‑1 (MQ=255)                              aaTATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACt                                                                                              >  2:359371/1‑140 (MQ=255)                              aaTATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACt                                                                                              <  2:521231/140‑1 (MQ=255)                              aaTATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACt                                                                                              >  1:173654/1‑140 (MQ=255)                                tatTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTgg                                                                                            >  2:463244/1‑140 (MQ=255)                                tatTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTgg                                                                                            <  2:775396/140‑1 (MQ=255)                                tatTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTgg                                                                                            <  1:128695/140‑1 (MQ=255)                                    gCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGgaaga                                                                                        <  2:337988/140‑1 (MQ=255)                                      aCTAACAAACGGCAGCCAGAGGGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGaagaag                                                                                      <  1:627511/140‑1 (MQ=255)                                      aCTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGaagaag                                                                                      >  1:779487/1‑140 (MQ=255)                                        tAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGAt                                                                                    >  2:98557/1‑140 (MQ=255)                                        tAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGAt                                                                                    <  1:572929/140‑1 (MQ=255)                                          aCAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCt                                                                                  >  1:783206/1‑140 (MQ=255)                                          aCAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTCAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCt                                                                                  >  1:758127/1‑140 (MQ=255)                                                    gCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGaagaag                                                                                      <  1:417574/126‑1 (MQ=255)                                                    gCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGaagaag                                                                                      >  2:417574/1‑126 (MQ=255)                                                     ccAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTg                                                                       >  1:387914/1‑140 (MQ=255)                                                       agagCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTgcg                                                                     <  1:98557/140‑1 (MQ=255)                                                         agCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGcgcg                                                                   >  2:215035/1‑140 (MQ=255)                                                            gcgTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTcc                                                                <  2:783206/140‑1 (MQ=255)                                                            gcgTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTcc                                                                <  1:361206/140‑1 (MQ=255)                                                            gcgTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTcc                                                                <  2:277921/140‑1 (MQ=255)                                                                   tCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATAtt                                                         >  1:750483/1‑140 (MQ=255)                                                                            ttAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGaa                                                      <  1:773861/134‑1 (MQ=255)                                                                            ttAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGaa                                                      >  2:773861/1‑134 (MQ=255)                                                                               aCCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCg                                             <  2:387914/140‑1 (MQ=255)                                                                                 cTGTTCGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGa                                           >  1:533664/1‑140 (MQ=255)                                                                                    tttGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAgg                                        >  2:12972/1‑140 (MQ=255)                                                                                    tttGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAgg                                        >  1:337422/1‑140 (MQ=255)                                                                                      tGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAGGcc                                      <  2:173654/140‑1 (MQ=255)                                                                                      tGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAGGcc                                      <  2:83731/140‑1 (MQ=255)                                                                                              gCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAGGCCAGTTTaaa                              >  2:423486/1‑140 (MQ=255)                                                                                               ccGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAGGCCAGTTTAAAt                             <  2:779487/140‑1 (MQ=255)                                                                                                             gggTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAGGCCAGTTTAAATACCACGTCGAtttt               <  1:463244/140‑1 (MQ=255)                                                                                                              ggTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAGGCCAGTTTAAATACCACGTCGATTTTg              <  2:758127/140‑1 (MQ=255)                                                                                                               gTCAAAAAAGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAGGCCAGTTTAAATACCACGTCGATTTTGa             <  1:686144/140‑1 (MQ=255)                                                                                                                   aaaaaGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAGGCCAGTTTAAATACCACGTCGATTTTGAGCAt         <  1:99084/140‑1 (MQ=255)                                                                                                                   aaaaaGTGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAGGCCAGTTTAAATACCACGTCGATTTTGAGCAt         <  2:934859/140‑1 (MQ=255)                                                                                                                         tGCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAGGCCAGTTTAAATACCACGTCGATTTTGAGCATCTGCat   >  1:720283/1‑140 (MQ=255)                                                                                                                          gCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAGGCCAGTTTAAATACCACGTCGATTTTGAGCATCTGCata  <  1:367075/140‑1 (MQ=255)                                                                                                                          gCTGTTCCCGCTTGCGCCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAGGCCAGTTTAAATACCACGTCGATTTTGAGCATCTGCata  <  2:983275/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                             AGAAGTTGGGTTGGGATATCGAACCACAGAATATTGCACTAACAAACGGCAGCCAGAGCGCGTTTTTCTACTTATTTAACCTGTTTGCCGGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCACCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAGGCCAGTTTAAATACCACGTCGATTTTGAGCATCTGCATA  >  NC_000913/3739970‑3740230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑