Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,827,726	T→C	T314A (ACC→GCC)	gltS ←	glutamate:sodium symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,827,726	0	T	C	100.0%	114.3 / NA	33	T314A (ACC→GCC)	gltS	glutamate:sodium symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/13);  total (20/13)

GCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGCCAGCACTGCCGCATCGTAGTTTTTGCCCATCATGCGCCAGGTAACGAAGATGGCATACAACGCCATGAAGATGGTCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGCTTACGTTACCCAGCACGGATACCGCACGCTCAAAGACGCG  >  NC_000913/3827590‑3827861                                                                                                                                         |                                                                                                                                        gCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGCCAGCACTGCCGCATCGTAGTTTTTGCCCATCATGCGCCAGGTAACGAAGATGGCATACAACGCCATGAAGATGGCCTg                                                                                                                                      >  1:303922/1‑140 (MQ=255)      cATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGCCAGCACTGCCGCATCGTAGTTTTTGCCCATCATGCGCCAGGTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTacca                                                                                                                                 >  1:670822/1‑140 (MQ=255)                aTTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGCCAGCACTGCCGCATCGTAGTTTTTGCCCATCATGCGCCAGGTAACGAAGATGGCATACATCGCCATGAAGATGGCCTGTACCACCAGGCTCGc                                                                                                                       <  2:4120/140‑1 (MQ=255)                 ttGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGCCAGCACTGCCGCATCGTAGTTTTTGCCCATCATGCGCCAGGTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGcc                                                                                                                      <  2:633823/140‑1 (MQ=255)                  tGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGCCAGCACTGCCGCATCGTAGTTTTTGCCCATCATGCGCCAGGTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCa                                                                                                                     >  2:914258/1‑140 (MQ=255)                     cGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGCCAGCACTGCCGCATCGTAGTTTTTGCCCATCATGCGCCAGGTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCa                                                                                                                  >  2:227012/1‑140 (MQ=255)                                    gAGGCCAAAACCACAGTGACCCGCAGCCAGCACTGCCGCATCGTAGTTTTTGCCCATCATGCGCCAGGTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCg                                                                                                   >  1:150242/1‑140 (MQ=255)                                    gAGGCCAAAACCACAGTGACCCGCAGCCAGCACTGCCGCATCGTAGTTTTTGCCCATCATGCGCCAGGTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCg                                                                                                   <  2:685016/140‑1 (MQ=255)                                     aGGCCAAAACCACAGTGACCCGCAGCCAGCACTGCCGCATCGTAGTTTTTGCCCATCATGCGCCAGGTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGa                                                                                                  >  2:454400/1‑140 (MQ=255)                                                 caGTGACCCGCAGCCAGCACTGCCGCATCGTAGTTTTTGCCCATCATGCGCCAGGTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCca                                                                                      <  2:259664/140‑1 (MQ=255)                                                        ccGCAGCCAGCACTGCCGCATCGTAGTTTTTGCCCATCATGCGCCAGGTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTc                                                                               >  2:191557/1‑140 (MQ=255)                                                                       ccGCATCGTAGTTTTTGCCCATCATGCGCCAGGTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGcc                                                                >  1:81242/1‑140 (MQ=255)                                                                       ccGCATCGTAGTTTTTGCCCATCATGCGCCAGGTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGcc                                                                >  1:884564/1‑140 (MQ=255)                                                                            tCGTAGTTTTTGCCCATCATGCGCCAGGTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGc                                                           >  2:386027/1‑140 (MQ=255)                                                                                        cccATCATGCGCCAGGTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGa                                               <  1:454400/140‑1 (MQ=255)                                                                                            tcatGCGCCAGGTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAg                                           >  2:481657/1‑140 (MQ=255)                                                                                                      ggTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGCTTACGTTAc                                 >  1:384138/1‑140 (MQ=255)                                                                                                       gTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGc                                          >  1:925804/1‑130 (MQ=255)                                                                                                       gTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGc                                          <  2:925804/130‑1 (MQ=255)                                                                                                       gTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGCTTACGTTAcc                                >  2:639988/1‑140 (MQ=255)                                                                                                       gTAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGCTTACGTTAcc                                >  2:406220/1‑140 (MQ=255)                                                                                                        tAACGAAGATGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGCTTACGTTAccc                               <  1:227012/140‑1 (MQ=255)                                                                                                                aTGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGCTTACGTTACCCAGCACGGa                       >  1:730874/1‑140 (MQ=255)                                                                                                                 tGGCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGCTTACGTTACCCAGCACGGAt                      >  2:94217/1‑140 (MQ=255)                                                                                                                  ggCATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGCTTACGTTACCCAGCACGGATa                     <  1:94217/140‑1 (MQ=255)                                                                                                                    cATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGCTTACGTTACCCAGCa                           >  2:446851/1‑132 (MQ=255)                                                                                                                    cATACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGCTTACGTTACCCAGCa                           <  1:446851/132‑1 (MQ=255)                                                                                                                     aTACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGCTTACGTTACCCAGCACGGATACCg                  <  2:384138/140‑1 (MQ=255)                                                                                                                      tACAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGCTTACGTTACCCAGCACGGATACCGc                 <  2:81242/140‑1 (MQ=255)                                                                                                                       aCAACGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGCTTACGTTACCCAGCACGGATACCGCa                <  2:884564/140‑1 (MQ=255)                                                                                                                          aCGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGCTTACGTTACCCAGCACGGATACCGCACg              <  2:813265/139‑1 (MQ=255)                                                                                                                          aCGCCATGAAGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGCTTACGTTACCCAGCACGGATACCGCACg              >  1:813265/1‑139 (MQ=255)                                                                                                                                   aGATGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGCTTACGTTACCCAGCACGGATACCGCACGCTCAAAGAcg    <  2:17756/140‑1 (MQ=255)                                                                                                                                     aTGGCCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGCTTACGTTACCCAGCACGGATACCGCACGCTCAAAGAcgcg  >  2:750811/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGCCAGCACTGCCGCATCGTAGTTTTTGCCCATCATGCGCCAGGTAACGAAGATGGCATACAACGCCATGAAGATGGTCTGTACCACCAGAATCGCCAGCATCGGCAGCGCCAGCGAAGCCAGCTCCCACAGTTTCAGCCCCATCAACGCCATCGCCAGGAACAACGACAAGCTTACGTTACCCAGCACGGATACCGCACGCTCAAAGACGCG  >  NC_000913/3827590‑3827861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑