Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,854,055	T→C	V45A (GTG→GCG)	emrD →	multidrug efflux pump EmrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,854,055	0	T	C	100.0%	243.6 / NA	69	V45A (GTG→GCG)	emrD	multidrug efflux pump EmrD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/33);  total (36/33)

ATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGTGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCG  >  NC_000913/3853919‑3854189                                                                                                                                         |                                                                                                                                       aTAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCa                                                                                                                                     <  1:858339/140‑1 (MQ=255)          aGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAAt                                                                                                                            >  2:250780/1‑140 (MQ=255)          aGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAAt                                                                                                                            <  2:607625/140‑1 (MQ=255)          aGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAAt                                                                                                                            <  2:596008/140‑1 (MQ=255)                 gAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCt                                                                                                                     >  1:904666/1‑140 (MQ=255)                 gAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCt                                                                                                                     <  2:3968/140‑1 (MQ=255)                  aaaCGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCtt                                                                                                                    <  1:342070/140‑1 (MQ=255)                      gTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATct                                                                                                                >  2:604859/1‑140 (MQ=255)                      gTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATct                                                                                                                >  2:314720/1‑140 (MQ=255)                       tCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATctg                                                                                                               <  2:904666/140‑1 (MQ=255)                       tCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATctg                                                                                                               <  2:888946/140‑1 (MQ=255)                               ttATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTa                                                                                                       <  2:260885/140‑1 (MQ=255)                                 aTTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACg                                                                                                     >  1:989192/1‑140 (MQ=255)                                  ttGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACgg                                                                                                    <  1:314720/140‑1 (MQ=255)                                   tGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGgt                                                                                                   >  1:598712/1‑140 (MQ=255)                                       gTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGtct                                                                                               <  2:631107/140‑1 (MQ=255)                                         tGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTca                                                                                             <  1:33251/140‑1 (MQ=255)                                         tGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTca                                                                                             <  2:276065/140‑1 (MQ=255)                                          ggTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTcac                                                                                            >  1:819858/1‑140 (MQ=255)                                               tACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTg                                                                                       >  2:513489/1‑140 (MQ=255)                                                aCTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCt                                                                                        <  2:536431/138‑1 (MQ=255)                                                aCTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCt                                                                                        >  1:536431/1‑138 (MQ=255)                                                     tGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTAt                                                                                 >  2:276599/1‑140 (MQ=255)                                                      ggCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATg                                                                                >  1:208999/1‑140 (MQ=255)                                                         cGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTACTGACTTACGGTGTCTCACAGCTGTTTTATGGcc                                                                             <  1:604859/140‑1 (MQ=255)                                                           tCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCg                                                                           >  2:763265/1‑140 (MQ=255)                                                            cGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGa                                                                          >  1:863668/1‑140 (MQ=255)                                                                   aTGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGa                                                                   >  1:250901/1‑140 (MQ=255)                                                                     ggCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGAcc                                                                 >  1:407475/1‑140 (MQ=255)                                                                       cgcAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGcc                                                                             <  1:790603/126‑1 (MQ=255)                                                                       cgcAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGcc                                                                             <  1:292332/126‑1 (MQ=255)                                                                       cgcAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGcc                                                                             >  2:292332/1‑126 (MQ=255)                                                                       cgcAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGcc                                                                             >  2:790603/1‑126 (MQ=255)                                                                        gcAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACcgcg                                                              >  2:565149/1‑140 (MQ=255)                                                                             ccATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGt                                                             >  2:465477/1‑136 (MQ=255)                                                                             ccATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGt                                                             <  1:465477/136‑1 (MQ=255)                                                                                   atatTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGa                                                   <  2:598712/140‑1 (MQ=255)                                                                                        ccAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGccgccg                                                    <  1:347981/134‑1 (MQ=255)                                                                                        ccAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGccgccg                                                    >  2:347981/1‑134 (MQ=255)                                                                                        ccAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGt                                              >  1:747794/1‑140 (MQ=255)                                                                                           gCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGAt                                           >  1:751587/1‑140 (MQ=255)                                                                                           gCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGAt                                           >  1:356718/1‑140 (MQ=255)                                                                                           gCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGAt                                           >  1:422267/1‑140 (MQ=255)                                                                                              aTTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCt                                        >  2:731586/1‑140 (MQ=255)                                                                                                     atatGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGa                                 <  2:863668/140‑1 (MQ=255)                                                                                                     atatGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGa                                 <  1:276599/140‑1 (MQ=255)                                                                                                        tGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATg                              <  1:763265/140‑1 (MQ=255)                                                                                                         ggCGCGCGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGt                             <  1:250780/140‑1 (MQ=255)                                                                                                          gcgcgcGATCTCAACGTCCGTGAAGGGGCGGCGTAGAGCGTAATGGGCGCTTATCTGCTGACTTTCGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTc                            <  2:796706/140‑1 (MQ=255)                                                                                                          gcgcgcGATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTc                            <  1:847845/140‑1 (MQ=255)                                                                                                             cgcgATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGa                                 <  1:495674/132‑1 (MQ=255)                                                                                                             cgcgATCTCAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGa                                 >  2:495674/1‑132 (MQ=255)                                                                                                                  tctcAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTa                    <  2:208999/140‑1 (MQ=255)                                                                                                                   ctcAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTAt                   <  2:250901/140‑1 (MQ=255)                                                                                                                    tcAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATg                  >  2:829332/1‑140 (MQ=255)                                                                                                                    tcAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATg                  <  1:849215/140‑1 (MQ=255)                                                                                                                     cAACGTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGc                 <  1:731586/140‑1 (MQ=255)                                                                                                                         gTCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGc             <  2:422267/140‑1 (MQ=255)                                                                                                                          tCCGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCa            >  1:936101/1‑140 (MQ=255)                                                                                                                            cGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTc                            >  1:831114/1‑122 (MQ=255)                                                                                                                            cGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTc                            <  2:831114/122‑1 (MQ=255)                                                                                                                            cGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAAc          >  2:548877/1‑140 (MQ=255)                                                                                                                            cGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAAc          >  2:724100/1‑140 (MQ=255)                                                                                                                            cGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAAc          >  1:99964/1‑140 (MQ=255)                                                                                                                            cGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAAc          >  1:754695/1‑140 (MQ=255)                                                                                                                            cGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAAc          >  1:233895/1‑140 (MQ=255)                                                                                                                            cGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAAc          >  1:194678/1‑140 (MQ=255)                                                                                                                            cGTGAAGGGGCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAAc          >  2:911684/1‑140 (MQ=255)                                                                                                                                  ggggCGGCGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGt    <  1:829332/140‑1 (MQ=255)                                                                                                                                    ggcggcGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTcg  >  1:735720/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       ATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATATTCCAGCTATTGCCGATATGGCGCGCGATCTCAACGTCCGTGAAGGGGCGGTGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCG  >  NC_000913/3853919‑3854189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑