Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	554,180	T→C	A86A (GCA→GCG)	ppiB ←	peptidyl‑prolyl cis‑trans isomerase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	554,180	0	T	C	100.0%	241.9 / NA	68	A86A (GCA→GCG)	ppiB	peptidyl‑prolyl cis‑trans isomerase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/36);  total (32/36)

TCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGT  >  NC_000913/554046‑554313                                                                                                                                       |                                                                                                                                      tCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCAt                                                                                                                                  <  2:828326/140‑1 (MQ=255)    aTGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGc                                                                                                                               <  2:409303/140‑1 (MQ=255)    aTGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGc                                                                                                                               <  1:272250/140‑1 (MQ=255)      gCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCa                                                                                                                             >  2:249185/1‑140 (MQ=255)        cGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGc                                                                                                                           <  2:265706/140‑1 (MQ=255)                 cTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACgg                                                                                                                  >  2:945388/1‑140 (MQ=255)                         aaCACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCATGGGTAttttt                                                                                                          >  2:865165/1‑140 (MQ=255)                          acacGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTc                                                                                                         <  1:865165/140‑1 (MQ=255)                            acGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGt                                                                                                                >  1:914849/1‑131 (MQ=255)                            acGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGt                                                                                                                <  2:914849/131‑1 (MQ=255)                             cGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGTAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAgg                                                                                                      <  2:823759/140‑1 (MQ=255)                             cGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAgg                                                                                                      <  1:249185/140‑1 (MQ=255)                                   aGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCgttg                                                                                                <  2:894205/140‑1 (MQ=255)                                    gCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCgttgt                                                                                               >  1:583691/1‑140 (MQ=255)                                     ccccAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCgttgtt                                                                                              >  1:882113/1‑140 (MQ=255)                                         aaCCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCt                                                                                          <  2:407070/140‑1 (MQ=255)                                          aCCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCtt                                                                                         >  1:385132/1‑140 (MQ=255)                                              tGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGtt                                                                                     >  2:988477/1‑140 (MQ=255)                                               gCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGttt                                                                                    <  1:945388/140‑1 (MQ=255)                                                cAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGtttt                                                                                   >  1:567203/1‑140 (MQ=255)                                                   gCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGa                                                                                >  2:494199/1‑140 (MQ=255)                                                    cTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGAt                                                                               <  1:824709/140‑1 (MQ=255)                                                    cTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGAt                                                                               <  1:494199/140‑1 (MQ=255)                                                    cTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGAt                                                                               <  1:988477/140‑1 (MQ=255)                                                              agaAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTtg                                                                     >  2:164177/1‑140 (MQ=255)                                                                       ggAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCtttt                                                            <  2:821912/140‑1 (MQ=255)                                                                               ttATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCAt                                                    >  1:171438/1‑140 (MQ=255)                                                                                  tCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGcc                                                 <  1:470117/140‑1 (MQ=255)                                                                                      ccACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGt                                             >  1:632924/1‑140 (MQ=255)                                                                                            tgatgaAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCaaaa                                       >  1:418348/1‑140 (MQ=255)                                                                                              atgaAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAAcc                                     >  1:154209/1‑140 (MQ=255)                                                                                               tgaAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAAccg                                    <  2:154209/140‑1 (MQ=255)                                                                                               tgaAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAAccg                                    <  2:632924/140‑1 (MQ=255)                                                                                                   gaaCTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAAccgc                                   >  1:448976/1‑137 (MQ=255)                                                                                                   gaaCTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAAccgc                                   <  2:448976/137‑1 (MQ=255)                                                                                                    aaCTGTGCAGTTGCAGAGTGCGGAGCCTGAGTCCGCGCCACTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGCTGGCTTTTTGTTTCATGCCCGGTTCAAAACCgccgcc                               <  2:882113/140‑1 (MQ=255)                                                                                                    aaCTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCgccgcc                               <  2:385132/140‑1 (MQ=255)                                                                                                    aaCTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCgccgcc                               <  2:414972/140‑1 (MQ=255)                                                                                                        gtgCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTg                            >  2:848347/1‑139 (MQ=255)                                                                                                        gtgCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTg                            <  1:848347/139‑1 (MQ=255)                                                                                                         tgCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGaa                          <  2:583691/140‑1 (MQ=255)                                                                                                         tgCAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTAAAAACCGCCGCCCTGaa                          >  1:799668/1‑140 (MQ=255)                                                                                                           cAGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATCGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATc                        >  1:553764/1‑140 (MQ=255)                                                                                                            aGTTGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTAAAAACCGCCGCCCTGAATCa                       <  2:799668/140‑1 (MQ=255)                                                                                                              ttGCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATa                     <  1:164177/140‑1 (MQ=255)                                                                                                              ttGCAGAGTGCGGAGCCTGAGTACGCGCCATCGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATa                     <  2:553764/140‑1 (MQ=255)                                                                                                                gCAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATaaa                   >  2:872144/1‑140 (MQ=255)                                                                                                                 cAGAGTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAg                  <  1:944918/140‑1 (MQ=255)                                                                                                                   gagTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAg                  <  1:115268/138‑1 (MQ=255)                                                                                                                   gagTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAg                  >  2:115268/1‑138 (MQ=255)                                                                                                                   gagTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGcc                <  1:641402/140‑1 (MQ=255)                                                                                                                   gagTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGcc                >  2:641402/1‑140 (MQ=255)                                                                                                                     gTGCGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGt              >  2:453276/1‑140 (MQ=255)                                                                                                                        cGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTTTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGa           >  2:997992/1‑140 (MQ=255)                                                                                                                        cGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAg                  >  2:765133/1‑133 (MQ=255)                                                                                                                        cGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAg                  <  1:765133/133‑1 (MQ=255)                                                                                                                        cGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGa           >  2:256273/1‑140 (MQ=255)                                                                                                                        cGGAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGa           >  1:872158/1‑140 (MQ=255)                                                                                                                         ggAGCCTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGAt          <  2:567203/140‑1 (MQ=255)                                                                                                                             ccTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAaca      >  2:1514/1‑140 (MQ=255)                                                                                                                             ccTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAaca      <  2:127613/140‑1 (MQ=255)                                                                                                                             ccTGAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAaca      <  1:453276/140‑1 (MQ=255)                                                                                                                                gAGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACgg   >  1:313587/1‑140 (MQ=255)                                                                                                                                 aGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGt  >  2:617913/1‑140 (MQ=255)                                                                                                                                 aGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGt  >  2:168531/1‑140 (MQ=255)                                                                                                                                 aGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGt  <  1:256273/140‑1 (MQ=255)                                                                                                                                 aGTACGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGt  >  1:134974/1‑140 (MQ=255)                                                                                                                                    aCGCGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGc                 <  2:641386/122‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      TCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGT  >  NC_000913/554046‑554313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑