Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,932,741	A→G	T476A (ACC→GCC)	kup →	K(+):H(+) symporter Kup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,932,741	0	A	G	100.0%	303.3 / NA	85	T476A (ACC→GCC)	kup	K(+):H(+) symporter Kup
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/47);  total (38/47)

TACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGT  >  NC_000913/3932605‑3932877                                                                                                                                         |                                                                                                                                         tACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCcg                                                                                                                                       <  2:790190/140‑1 (MQ=255) tACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCcg                                                                                                                                       >  2:445057/1‑140 (MQ=255) tACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCcg                                                                                                                                       <  2:525437/140‑1 (MQ=255)  aCTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGTATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCcgc                                                                                                                                      <  1:668517/140‑1 (MQ=255)  aCTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCcgc                                                                                                                                      <  1:579135/140‑1 (MQ=255)  aCTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCcgc                                                                                                                                      <  1:888960/140‑1 (MQ=255)  aCTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCcgc                                                                                                                                      <  2:365865/140‑1 (MQ=255)   cTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCcg                                                                                                                                       <  1:572408/138‑1 (MQ=255)   cTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCcg                                                                                                                                       >  2:572408/1‑138 (MQ=255)    tgtgATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCgg                                                                                                                                    <  1:960419/140‑1 (MQ=255)    tgtgATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCgg                                                                                                                                    <  2:837053/140‑1 (MQ=255)      tgATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGgtg                                                                                                                                  >  2:88483/1‑140 (MQ=255)        aTGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGta                                                                                                                                >  1:398953/1‑140 (MQ=255)           tttATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCcgc                                                                                                                                      <  1:264646/131‑1 (MQ=255)           tttATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCcgc                                                                                                                                      >  2:264646/1‑131 (MQ=255)           tttATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTatat                                                                                                                             >  2:237830/1‑140 (MQ=255)           tttATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTatat                                                                                                                             >  1:840579/1‑140 (MQ=255)           tttATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTatat                                                                                                                             >  2:983542/1‑140 (MQ=255)           tttATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTatat                                                                                                                             >  2:186995/1‑140 (MQ=255)             tATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGt                                                                                                                           <  2:398953/140‑1 (MQ=255)             tATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGt                                                                                                                           >  1:712556/1‑140 (MQ=255)                cGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCcg                                                                                                                                       >  1:583184/1‑125 (MQ=255)                cGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCcg                                                                                                                                       <  2:583184/125‑1 (MQ=255)                cGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTcgc                                                                                                                        >  2:655140/1‑140 (MQ=255)                 gTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTcgcg                                                                                                                       <  1:88483/140‑1 (MQ=255)                 gTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTcgcg                                                                                                                       <  2:490715/140‑1 (MQ=255)                   gatgaCCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTg                                                                                                                     <  2:424109/140‑1 (MQ=255)                    atgaCCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCTGGGCCGCGGTGTATATGTCGCGTGc                                                                                                                    >  1:880937/1‑140 (MQ=255)                    atgaCCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGc                                                                                                                    >  1:552543/1‑140 (MQ=255)                         caccTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCa                                                                                                               <  2:675353/140‑1 (MQ=255)                         caccTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCa                                                                                                               <  2:668025/140‑1 (MQ=255)                         caccTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCa                                                                                                               >  2:64538/1‑140 (MQ=255)                           ccTGGAAGAGCGAGCGTTTCCGCTTGCTTCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAAc                                                                                                             >  2:109516/1‑140 (MQ=255)                            cTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACg                                                                                                            >  1:562845/1‑140 (MQ=255)                                aaGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCAt                                                                                                        <  1:445057/140‑1 (MQ=255)                                aaGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCAt                                                                                                        <  2:552543/140‑1 (MQ=255)                                   agcgagcgTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTcc                                                                                                     >  1:463664/1‑140 (MQ=255)                                    gcgagcgTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTccc                                                                                                    >  1:195701/1‑140 (MQ=255)                                      gagcgTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTACGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCtt                                                                                                  >  1:595424/1‑140 (MQ=255)                                          gTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTACGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTgcg                                                                                              <  2:595424/140‑1 (MQ=255)                                           tttCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTgcgc                                                                                             <  2:873311/140‑1 (MQ=255)                                            ttCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCt                                                                                            <  1:64538/140‑1 (MQ=255)                                             tCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTg                                                                                           >  1:666735/1‑140 (MQ=255)                                                   tgctgcGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCAt                                                                                     <  1:237830/140‑1 (MQ=255)                                                   tgctgcGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCAt                                                                                     <  2:109937/140‑1 (MQ=255)                                                    gctgcGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATa                                                                                    <  2:195701/140‑1 (MQ=255)                                                     ctgcGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATaa                                                                                   >  1:937424/1‑140 (MQ=255)                                                      tgcGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAAc                                                                                  >  2:261010/1‑140 (MQ=255)                                                          gcggATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTa                                                                              <  1:261010/140‑1 (MQ=255)                                                            ggATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTaaa                                                                            <  1:952847/140‑1 (MQ=255)                                                              aTGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACa                                                                          <  2:712556/140‑1 (MQ=255)                                                                   tGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACa                                                                     <  1:655140/140‑1 (MQ=255)                                                                    gAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACaa                                                                    <  2:666735/140‑1 (MQ=255)                                                                          ggTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTAtt                                                              >  2:320728/1‑140 (MQ=255)                                                                          ggTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTAtt                                                              >  2:245505/1‑140 (MQ=255)                                                                          ggTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTAtt                                                              >  1:485852/1‑140 (MQ=255)                                                                          ggTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTAtt                                                              >  2:723983/1‑140 (MQ=255)                                                                                tctctGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCTGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGa                                                        >  2:449494/1‑140 (MQ=255)                                                                                 ctctGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAg                                                       <  2:463664/140‑1 (MQ=255)                                                                                    tGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCgg                                                    >  1:392125/1‑140 (MQ=255)                                                                                    tGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCgg                                                    >  2:269438/1‑140 (MQ=255)                                                                                     ggAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCggg                                                   >  2:838649/1‑140 (MQ=255)                                                                                     ggAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCggg                                                   <  2:840579/140‑1 (MQ=255)                                                                                       aaGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTg                                                 <  1:186995/140‑1 (MQ=255)                                                                                         gCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCTGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGAt                                               <  1:449494/140‑1 (MQ=255)                                                                                         gCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCTGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGAt                                               <  2:880937/140‑1 (MQ=255)                                                                                          cGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGAtt                                              >  2:841016/1‑140 (MQ=255)                                                                                            atgatTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCt                                            >  2:751502/1‑140 (MQ=255)                                                                                                      cGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTg                                  <  1:320728/140‑1 (MQ=255)                                                                                                      cGCTGGAGAAACCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTg                                  <  1:245505/140‑1 (MQ=255)                                                                                                        cTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTgcg                                <  1:269438/140‑1 (MQ=255)                                                                                                             gaAATCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCg                           <  1:109516/140‑1 (MQ=255)                                                                                                               aaTCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGaa                         <  1:841016/140‑1 (MQ=255)                                                                                                               aaTCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGaa                         <  1:751502/140‑1 (MQ=255)                                                                                                               aaTCACCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGaa                         <  2:937424/140‑1 (MQ=255)                                                                                                                aTCACCGCCTGTTCGCGTGACCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAg                        >  1:541889/1‑140 (MQ=255)                                                                                                                 tCACCGCCTGTTCGCGTGACCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGa                       <  2:541889/140‑1 (MQ=255)                                                                                                                   aCCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTgcg                                <  2:764545/129‑1 (MQ=255)                                                                                                                   aCCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTgcg                                >  1:764545/1‑129 (MQ=255)                                                                                                                   aCCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACg                     <  1:723983/140‑1 (MQ=255)                                                                                                                   aCCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACg                     >  2:67596/1‑140 (MQ=255)                                                                                                                   aCCGCCTGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCAGGTGATTCTGTTAACTCTGCGCACCGAAGACg                     >  2:528488/1‑140 (MQ=255)                                                                                                                         tGTTCGCGTGCCCGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCat               <  1:67596/140‑1 (MQ=255)                                                                                                                                   cccGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGa                       <  1:784658/122‑1 (MQ=255)                                                                                                                                   cccGGGGCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGa                       >  2:784658/1‑122 (MQ=255)                                                                                                                                      ggggCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAAcgt  <  1:983542/140‑1 (MQ=255)                                                                                                                                      ggggCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAAcgt  <  2:392125/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGT  >  NC_000913/3932605‑3932877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑