Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,973,620	A→G	I5V (ATC→GTC)	rffH →	dTDP‑glucose pyrophosphorylase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,973,620	0	A	G	100.0%	263.9 / NA	74	I5V (ATC→GTC)	rffH	dTDP‑glucose pyrophosphorylase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/35);  total (39/35)

GCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATT  >  NC_000913/3973493‑3973754                                                                                                                                |                                                                                                                                       gCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTggcgggcg                                                                                                                            <  1:919177/140‑1 (MQ=255)    tAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGtt                                                                                                                         >  1:697879/1‑140 (MQ=255)     aaaaCGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTc                                                                                                                        >  1:110580/1‑140 (MQ=255)       aaCGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCg                                                                                                                      <  2:110580/140‑1 (MQ=255)       aaCGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCg                                                                                                                      >  1:787551/1‑140 (MQ=255)        aCGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCgg                                                                                                                     <  1:435657/140‑1 (MQ=255)            ttCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTGCGGCAcc                                                                                                                 >  2:830662/1‑140 (MQ=255)                 tGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTggcgggcg                                                                                                                            <  1:1001443/124‑1 (MQ=255)                 tGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTggcgggcg                                                                                                                            >  2:1001443/1‑124 (MQ=255)                 tGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGAtt                                                                                                            >  2:216352/1‑140 (MQ=255)                 tGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGAtt                                                                                                            <  2:58062/140‑1 (MQ=255)                    tATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCa                                                                                                                   >  1:516987/1‑130 (MQ=255)                    tATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCa                                                                                                                   <  2:516987/130‑1 (MQ=255)                      tCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATc                                                                                                       <  2:339309/140‑1 (MQ=255)                      tCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATc                                                                                                       >  1:878037/1‑140 (MQ=255)                      tCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATc                                                                                                       >  1:219606/1‑140 (MQ=255)                      tCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATc                                                                                                       >  2:641565/1‑140 (MQ=255)                           cTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGAtt                                                                                                  >  1:305174/1‑140 (MQ=255)                           cTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGAtt                                                                                                  >  1:341825/1‑140 (MQ=255)                           cTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGAtt                                                                                                  >  2:515389/1‑140 (MQ=255)                              aTGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTAcg                                                                                               <  1:716028/140‑1 (MQ=255)                              aTGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGACTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTAcg                                                                                               >  1:69875/1‑140 (MQ=255)                                    gCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTAcgcgcg                                                                                           >  1:643768/1‑138 (MQ=255)                                    gCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTAcgcgcg                                                                                           <  2:643768/138‑1 (MQ=255)                                        gtggAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTAcgcg                                                                                             <  1:655351/132‑1 (MQ=255)                                        gtggAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTAcgcg                                                                                             >  2:655351/1‑132 (MQ=255)                                        gtggAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTAt                                                                                     >  2:359288/1‑140 (MQ=255)                                        gtggAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTAt                                                                                     >  2:488626/1‑140 (MQ=255)                                         tggAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATc                                                                                    <  1:305864/140‑1 (MQ=255)                                             aGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAg                                                                                <  2:878037/140‑1 (MQ=255)                                              gCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTAcg                                                                                               <  1:355614/124‑1 (MQ=255)                                              gCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTAcg                                                                                               >  2:355614/1‑124 (MQ=255)                                              gCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGc                                                                               <  1:830662/140‑1 (MQ=255)                                                  gTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACt                                                                           >  2:595897/1‑140 (MQ=255)                                                  gTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACt                                                                           >  2:580675/1‑140 (MQ=255)                                                     cAGGGCGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGtt                                                                        >  1:678614/1‑140 (MQ=255)                                                      aGGGCGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTg                                                                       <  2:678614/140‑1 (MQ=255)                                                      aGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTg                                                                       >  2:161355/1‑140 (MQ=255)                                                       ggACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGc                                                                      <  1:247750/140‑1 (MQ=255)                                                       ggACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGc                                                                      <  2:660703/140‑1 (MQ=255)                                                       ggACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGc                                                                      <  1:161355/140‑1 (MQ=255)                                                           ggCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGAt                                                                  <  2:219606/140‑1 (MQ=255)                                                            gCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGAtt                                                                 <  1:377872/140‑1 (MQ=255)                                                             cAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGAttt                                                                >  2:795875/1‑140 (MQ=255)                                                             cAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGAttt                                                                >  1:621779/1‑140 (MQ=255)                                                                     aGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAg                                                        >  1:17278/1‑140 (MQ=255)                                                                      gggCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGc                                                       <  2:787551/140‑1 (MQ=255)                                                                      gggCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGc                                                       <  1:488626/140‑1 (MQ=255)                                                                        gCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCa                                                     <  2:697879/140‑1 (MQ=255)                                                                        gCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCa                                                     <  1:157903/140‑1 (MQ=255)                                                                          gAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCa                                                     >  2:271244/1‑138 (MQ=255)                                                                          gAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCa                                                     <  1:271244/138‑1 (MQ=255)                                                                           aGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATg                                                  >  2:292120/1‑140 (MQ=255)                                                                           aGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATg                                                  >  1:333143/1‑140 (MQ=255)                                                                             cGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGAt                                                >  1:117282/1‑140 (MQ=255)                                                                               tttAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGAttt                                              <  1:359288/140‑1 (MQ=255)                                                                                 tAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCa                                                     <  2:526015/131‑1 (MQ=255)                                                                                 tAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCa                                                     >  1:526015/1‑131 (MQ=255)                                                                                         aaaGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCt                                    >  2:327037/1‑140 (MQ=255)                                                                                           aGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGt                                  <  2:333143/140‑1 (MQ=255)                                                                                            ggCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTc                                 <  2:621779/140‑1 (MQ=255)                                                                                              cTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTc                                 >  1:607806/1‑138 (MQ=255)                                                                                              cTAATTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTc                                 <  2:607806/138‑1 (MQ=255)                                                                                                aaTTTTCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTg                             <  1:795875/140‑1 (MQ=255)                                                                                                     tCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGAt                        <  1:580675/140‑1 (MQ=255)                                                                                                     tCAGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGAt                        <  2:341825/140‑1 (MQ=255)                                                                                                       aGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATcc                                       >  2:477117/1‑123 (MQ=255)                                                                                                       aGCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATcc                                       <  1:477117/123‑1 (MQ=255)                                                                                                        gCGGAGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCt                     >  2:68806/1‑140 (MQ=255)                                                                                                            aGGCAAACATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGcc                 >  2:329630/1‑140 (MQ=255)                                                                                                                   cATGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATcc          >  1:10092/1‑140 (MQ=255)                                                                                                                    aTGAAAGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCcg         <  2:981208/140‑1 (MQ=255)                                                                                                                        aaGGTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGaa     >  2:251577/1‑140 (MQ=255)                                                                                                                           gTATTGTCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAAtt  >  2:393316/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                       GCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATT  >  NC_000913/3973493‑3973754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑