Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,165,198	A→G	K520K (AAA→AAG)	btuB →	cobalamin/cobinamide outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,165,198	0	A	G	100.0%	293.6 / NA	82	K520K (AAA→AAG)	btuB	cobalamin/cobinamide outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/48);  total (34/48)

AACGAAGGGAAAGCGCGGATTAAGGGCGTCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAACAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTACTCATCTTATCCTTATCAAACCGTTAAAATGGGCGGTGTGAG  >  NC_000913/4165061‑4165332                                                                                                                                          |                                                                                                                                       aaCGAAGGGAAAGCGCGGATTAAGGGCGTCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGca                                                                                                                                      <  1:140880/140‑1 (MQ=255)  aCGAAGGGAAAGCGCGGATTAAGGGCGTCGAGGCGACCGCCAATTTTGATATCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGcag                                                                                                                                     >  1:793669/1‑140 (MQ=255)  aCGAAGGGAAAGCGCGGATTAAGGGCGTCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGcag                                                                                                                                     >  2:240747/1‑140 (MQ=255)   cGAAGGGAAAGCGCGGATTAAGGGCGTCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAagcagc                                                                                                                                    <  1:133137/140‑1 (MQ=255)   cGAAGGGAAAGCGCGGATTAAGGGCGTCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAagcagc                                                                                                                                    <  2:780767/140‑1 (MQ=255)   cGAAGGGAAAGCGCGGATTAAGGGCGTCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAagcagc                                                                                                                                    <  1:846445/140‑1 (MQ=255)    gAAGGGAAAGGGCGGATTAAGGGGGCGGGGGGGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAgcagca                                                                                                                                   <  2:189410/140‑1 (MQ=255)    gAAGGGAAAGCGCGGATTAAGGGCGTCGAGGCGACCGCCAATTTTGATACCGGACCACTGACACATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAgcagca                                                                                                                                   >  1:842958/1‑140 (MQ=255)        ggAAAGCGCGGATTAAGGGCGTCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTg                                                                                                                               >  1:926690/1‑140 (MQ=255)          aaaGCGCGGATTAAGGGCGTCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGaa                                                                                                                             >  2:994644/1‑140 (MQ=255)          aaaGCGCGGATTAAGGGCGTCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGaa                                                                                                                             >  1:521258/1‑140 (MQ=255)            agggcgGTTTAGGGGCGTCGAGGCGTCCGCCAATTTTGATCCCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAAt                                                                                                                           <  2:362147/137‑1 (MQ=255)                     tAAGGGCGTCGAGGCGCCCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAAGGCGATTACCGCCACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCg                                                                                                                  <  2:950389/140‑1 (MQ=255)                       aGGGCGTCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGAc                                                                                                                <  2:682768/140‑1 (MQ=255)                          gCGTCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTgg                                                                                                             <  2:569176/140‑1 (MQ=255)                            gTCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCa                                                                                                           >  2:949427/1‑140 (MQ=255)                            gTCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCa                                                                                                           >  1:571998/1‑140 (MQ=255)                             tCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAg                                                                                                          >  1:773645/1‑140 (MQ=255)                             tCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAg                                                                                                          >  1:491036/1‑140 (MQ=255)                             tCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAg                                                                                                          >  2:862799/1‑140 (MQ=255)                             tCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAg                                                                                                          >  2:227512/1‑140 (MQ=255)                             tCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAg                                                                                                          >  1:424756/1‑140 (MQ=255)                                 ggCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGt                                                                                                      <  1:596572/140‑1 (MQ=255)                                  gCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTa                                                                                                     <  1:227512/140‑1 (MQ=255)                                            aTTTTGATCCCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGAc                                                                                           <  2:722099/140‑1 (MQ=255)                                            aTTTTGATACCGGACCACTGACGCATACTGTTAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGAc                                                                                           <  2:426872/140‑1 (MQ=255)                                            aTTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGAc                                                                                           <  1:599976/140‑1 (MQ=255)                                            aTTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGAc                                                                                           <  2:55841/140‑1 (MQ=255)                                            aTTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGAc                                                                                           <  2:571998/140‑1 (MQ=255)                                              tttGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTg                                                                                         <  1:949427/140‑1 (MQ=255)                                                 gATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTgggg                                                                                      <  1:862799/140‑1 (MQ=255)                                                 gATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTgggg                                                                                      <  2:986297/140‑1 (MQ=255)                                                 gATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTgggg                                                                                      <  2:491036/140‑1 (MQ=255)                                                     ccGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTAtt                                                                                  >  2:785779/1‑140 (MQ=255)                                                        gACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACt                                                                               >  1:127553/1‑140 (MQ=255)                                                        gACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACt                                                                               >  1:853989/1‑140 (MQ=255)                                                        gACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACt                                                                               >  1:635308/1‑140 (MQ=255)                                                           cACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTTGGGTATTACTTAt                                                                            <  1:549465/140‑1 (MQ=255)                                                           cACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTAt                                                                            <  1:203668/140‑1 (MQ=255)                                                             cTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCa                                                                          <  2:635308/140‑1 (MQ=255)                                                              tGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAg                                                                         <  1:921537/140‑1 (MQ=255)                                                                   cATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTAttt                                                                    >  2:405470/1‑140 (MQ=255)                                                                     tACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAg                                                                  >  1:830234/1‑140 (MQ=255)                                                                      aCTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAgg                                                                 <  2:521462/140‑1 (MQ=255)                                                                      aCTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAgg                                                                 <  2:373053/140‑1 (MQ=255)                                                                        tgtgAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCa                                                               <  1:736452/140‑1 (MQ=255)                                                                          tgAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACt                                                             <  2:926690/140‑1 (MQ=255)                                                                          tgAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACt                                                             <  2:842958/140‑1 (MQ=255)                                                                          tgAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACt                                                             >  2:241226/1‑140 (MQ=255)                                                                          tgAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACt                                                             <  2:945805/140‑1 (MQ=255)                                                                          tgAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACt                                                             >  1:945805/1‑140 (MQ=255)                                                                          tgAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACt                                                             <  2:777709/140‑1 (MQ=255)                                                                          tgAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGACACt                                                             >  2:949539/1‑140 (MQ=255)                                                                           gAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTc                                                            >  2:61009/1‑140 (MQ=255)                                                                             gTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGc                                                          <  2:793669/140‑1 (MQ=255)                                                                              ttATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCt                                                         <  1:606441/140‑1 (MQ=255)                                                                                aTGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTAt                                                       >  1:831287/1‑140 (MQ=255)                                                                                   aTTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGAt                                                    <  2:249331/140‑1 (MQ=255)                                                                                   aTTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGAt                                                    <  2:127553/140‑1 (MQ=255)                                                                                   aTTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCCACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGAt                                                    <  2:521258/140‑1 (MQ=255)                                                                                      aTGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACt                                                             <  1:447474/128‑1 (MQ=255)                                                                                      aTGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACt                                                             >  2:447474/1‑128 (MQ=255)                                                                                      aTGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAg                                                 >  2:270760/1‑140 (MQ=255)                                                                                      aTGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAg                                                 <  2:926214/140‑1 (MQ=255)                                                                                      aTGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAg                                                 <  1:240747/140‑1 (MQ=255)                                                                                           gATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTa                                            >  1:947195/1‑140 (MQ=255)                                                                                            aTGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTAc                                           <  2:830234/140‑1 (MQ=255)                                                                                            aTGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTAc                                           >  2:966121/1‑140 (MQ=255)                                                                                            aTGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTAc                                           <  1:405470/140‑1 (MQ=255)                                                                                            aTGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTAc                                           <  1:678993/140‑1 (MQ=255)                                                                                            aTGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTAc                                           <  1:329572/140‑1 (MQ=255)                                                                                            aTGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTAc                                           <  1:241226/140‑1 (MQ=255)                                                                                            aTGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTAc                                           >  2:171610/1‑140 (MQ=255)                                                                                            aTGCGCGCAATGCGATTACCGACAAGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTTTATGACGTCGGCTGGGGTATTAGTTGTCGGTATTTAGGCCCTCGCTATGATAAGGATTAc                                           >  2:733533/1‑140 (MQ=255)                                                                                                 cgcAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTACTCATc                                      <  2:881921/140‑1 (MQ=255)                                                                                                   cAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTACTCATctt                                    >  1:7423/1‑140 (MQ=255)                                                                                                               cGACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTACTCATCTTATCCTTATCaaa                        <  2:947195/140‑1 (MQ=255)                                                                                                                gACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTACTCATCTTATCCTTATCAAAc                       <  1:270760/140‑1 (MQ=255)                                                                                                                gACACGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTACTCATCTTATCCTTATCAAAc                       >  2:930739/1‑140 (MQ=255)                                                                                                                  cacGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTACTCATCTTATCCTTATCAAACCg                     >  1:48432/1‑140 (MQ=255)                                                                                                                    cGCCGTTGTTACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTACTCATCTTATCCTTATCAAACCGtt                   <  2:7423/140‑1 (MQ=255)                                                                                                                            ttACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTACTCATCTTATCCTTATCAAACCGTTAAAATggg           <  1:733533/140‑1 (MQ=255)                                                                                                                            ttACGCCGTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTACTCATCTTATCCTTATCAAACCGTTAAAATggg           <  1:966121/140‑1 (MQ=255)                                                                                                                                   gTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTACTCATCTTATCCTTATCAAACCGTTAAAATGGGCGGtgtg    >  1:541113/1‑140 (MQ=255)                                                                                                                                   gTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTACTCATCTTATCCTTATCAAACCGTTAAAATGGGCGGtgtg    >  1:179884/1‑140 (MQ=255)                                                                                                                                   gTGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTACTCATCTTATCCTTATCAAACCGTTAAAATGGGCGGtgtg    <  2:541113/140‑1 (MQ=255)                                                                                                                                    tGCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTACTCATCTTATCCTTATCAAACCGTTAAAATGGGCGGTGTGa   >  1:544854/1‑140 (MQ=255)                                                                                                                                     gCTAAGCAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTACTCATCTTATCCTTATCAAACCGTTAAAATGGGCGGTGTGAg  <  2:179884/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       AACGAAGGGAAAGCGCGGATTAAGGGCGTCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGTTATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAACAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTATCAGTATTTAGGCACTCGCTATGATAAGGATTACTCATCTTATCCTTATCAAACCGTTAAAATGGGCGGTGTGAG  >  NC_000913/4165061‑4165332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑