Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,234,854	A→G	N366S (AAC→AGC)	pgi →	glucose‑6‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,234,854	0	A	G	100.0%	240.7 / NA	68	N366S (AAC→AGC)	pgi	glucose‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/35);  total (33/35)

GGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCAT  >  NC_000913/4234718‑4234987                                                                                                                                         |                                                                                                                                      ggCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGt                                                                                                                                    <  2:219156/140‑1 (MQ=255) ggCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGt                                                                                                                                    <  2:454096/140‑1 (MQ=255)   cATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTtg                                                                                                                                  <  2:236250/140‑1 (MQ=255)   cATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTtg                                                                                                                                  <  1:381952/140‑1 (MQ=255)     tCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTtgtg                                                                                                                                <  2:728384/140‑1 (MQ=255)     tCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACCGTAGCGTtgtg                                                                                                                                <  2:549156/140‑1 (MQ=255)        ggTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGAt                                                                                                                             >  1:294304/1‑140 (MQ=255)         gTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTtgtg                                                                                                                                >  1:226778/1‑136 (MQ=255)         gTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTtgtg                                                                                                                                <  2:226778/136‑1 (MQ=255)            caacaaTTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTAcc                                                                                                                         >  1:763429/1‑140 (MQ=255)               caaTTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGa                                                                                                                      >  2:993811/1‑140 (MQ=255)                         ggTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGAt                                                                                                            >  1:347167/1‑140 (MQ=255)                          gTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGAtt                                                                                                           >  1:267430/1‑140 (MQ=255)                                 aaCTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTggg                                                                                                    <  1:993811/140‑1 (MQ=255)                                      aaGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGaa                                                                                               <  2:347167/140‑1 (MQ=255)                                      aaGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGaa                                                                                               <  2:294304/140‑1 (MQ=255)                                                      tGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAgg                                                                                          <  2:692188/129‑1 (MQ=255)                                                      tGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAgg                                                                                          >  1:692188/1‑129 (MQ=255)                                                      tGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTc                                                                               >  1:781590/1‑140 (MQ=255)                                                      tGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTc                                                                               >  2:672672/1‑140 (MQ=255)                                                      tGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTc                                                                               >  2:162908/1‑140 (MQ=255)                                                          cAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCa                                                                           <  1:605749/140‑1 (MQ=255)                                                           aGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCAc                                                                          >  1:264239/1‑140 (MQ=255)                                                            gTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCAcg                                                                         <  1:474884/140‑1 (MQ=255)                                                             tataTGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCAcgc                                                                        <  2:763429/140‑1 (MQ=255)                                                              atatGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCAcgcg                                                                       <  2:879083/140‑1 (MQ=255)                                                                  gCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACg                                                                                  <  2:65575/125‑1 (MQ=255)                                                                  gCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACg                                                                                  >  1:65575/1‑125 (MQ=255)                                                                    aCCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTAc                                                                 >  1:530553/1‑140 (MQ=255)                                                                       gTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGTCTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCa                                                                           <  1:957108/127‑1 (MQ=255)                                                                       gTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGTCTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCa                                                                           >  2:957108/1‑127 (MQ=255)                                                                       gTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTc                                                                               <  2:735301/123‑1 (MQ=255)                                                                       gTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTc                                                                               >  1:735301/1‑123 (MQ=255)                                                                       gTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAg                                                              >  1:98193/1‑140 (MQ=255)                                                                       gTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAg                                                              >  2:6117/1‑140 (MQ=255)                                                                       gTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAg                                                              >  1:231587/1‑140 (MQ=255)                                                                       gTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAg                                                              >  2:138441/1‑140 (MQ=255)                                                                       gTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAg                                                              >  2:467279/1‑140 (MQ=255)                                                                       gTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAg                                                              >  1:920829/1‑140 (MQ=255)                                                                        tttCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCa                                                                           <  1:793929/126‑1 (MQ=255)                                                                        tttCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCa                                                                           >  2:793929/1‑126 (MQ=255)                                                                           cgcgGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGa                                                          >  2:989557/1‑140 (MQ=255)                                                                                    cTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAgg                                                 <  2:231587/140‑1 (MQ=255)                                                                                    cTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAgg                                                 <  1:630057/140‑1 (MQ=255)                                                                                        cagcagGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAAc                                             <  1:989557/140‑1 (MQ=255)                                                                                        cagcagGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAAc                                             <  1:535770/140‑1 (MQ=255)                                                                                        cagcagGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAAc                                             <  2:410279/140‑1 (MQ=255)                                                                                        cagcagGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAAc                                             <  2:530553/140‑1 (MQ=255)                                                                                          gcagGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCa                                           >  1:536225/1‑140 (MQ=255)                                                                                              ggCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAAc                                             >  1:484632/1‑134 (MQ=255)                                                                                              ggCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAAc                                             <  2:484632/134‑1 (MQ=255)                                                                                              ggCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAAt                                       >  2:82528/1‑140 (MQ=255)                                                                                              ggCAATATGGAGTCCAACGGCAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAAt                                       >  2:169391/1‑140 (MQ=255)                                                                                                   tatGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTAc                                  <  2:781590/140‑1 (MQ=255)                                                                                                          tCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGa                           <  2:264239/140‑1 (MQ=255)                                                                                                          tCCAACGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGa                           >  2:988519/1‑140 (MQ=255)                                                                                                               cGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCa                      >  2:868179/1‑140 (MQ=255)                                                                                                               cGGTAAGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCa                      >  2:399550/1‑140 (MQ=255)                                                                                                                    aGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCt                 <  2:98193/140‑1 (MQ=255)                                                                                                                    aGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCt                 <  2:215456/140‑1 (MQ=255)                                                                                                                    aGTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCt                 >  1:93106/1‑140 (MQ=255)                                                                                                                     gTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTc                <  2:536225/140‑1 (MQ=255)                                                                                                                     gTATGTTGACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTc                <  1:988519/140‑1 (MQ=255)                                                                                                                            gACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTAt         <  2:267430/140‑1 (MQ=255)                                                                                                                            gACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTAt         <  1:108465/140‑1 (MQ=255)                                                                                                                            gACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTAt         >  2:108465/1‑140 (MQ=255)                                                                                                                            gACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTAt         >  1:969518/1‑140 (MQ=255)                                                                                                                            gACCGTAACGGTAGCGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTAt         <  1:164759/140‑1 (MQ=255)                                                                                                                                gtaacggtagcgTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCAcc     >  2:692520/1‑140 (MQ=255)                                                                                                                                gtaacggtagcgTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCAcc     >  2:434969/1‑140 (MQ=255)                                                                                                                                gtaacggtagcgTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCAcc     >  2:143420/1‑140 (MQ=255)                                                                                                                                gtaacggtagcgTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCAcc     <  1:692520/140‑1 (MQ=255)                                                                                                                                  aacggtagcgTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCa   >  2:314402/1‑140 (MQ=255)                                                                                                                                   acggtagcgTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCAt  <  1:82528/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      GGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCAT  >  NC_000913/4234718‑4234987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑