Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,305,347	A→G	G417G (GGT→GGC)	yjcS ←	alkyl sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,305,347	0	A	G	100.0%	288.3 / NA	81	G417G (GGT→GGC)	yjcS	alkyl sulfatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/39);  total (42/39)

GGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGT  >  NC_000913/4305214‑4305483                                                                                                                                      |                                                                                                                                         gggCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTaa                                                                                                                                    >  2:906291/1‑140 (MQ=255) gggCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTaa                                                                                                                                    >  2:719235/1‑140 (MQ=255)            cccAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCt                                                                                                                         >  1:260635/1‑140 (MQ=255)            cccAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCt                                                                                                                         >  2:173118/1‑140 (MQ=255)             ccAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTg                                                                                                                        >  1:992288/1‑140 (MQ=255)               aGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGc                                                                                                                      <  2:260635/140‑1 (MQ=255)                gcgcCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGcc                                                                                                                     <  2:36862/140‑1 (MQ=255)                 cgcCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGccc                                                                                                                    <  1:744283/140‑1 (MQ=255)                   ccTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGcc                                                                                                                     <  1:152506/137‑1 (MQ=255)                   ccTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGcc                                                                                                                     >  2:152506/1‑137 (MQ=255)                   ccTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAg                                                                                                                  <  1:719235/140‑1 (MQ=255)                      gCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTa                                                                                                               >  2:260529/1‑140 (MQ=255)                                  tACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCa                                                                                                   >  1:592282/1‑140 (MQ=255)                                    cccATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAgg                                                                                                 >  2:479218/1‑140 (MQ=255)                                      cATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAggcg                                                                                               >  1:4302/1‑140 (MQ=255)                                       aTCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGTCCAGTTATTGGCAAGTGCAggcgg                                                                                              <  2:836613/140‑1 (MQ=255)                                       aTCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAggcgg                                                                                              <  2:4302/140‑1 (MQ=255)                                       aTCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAggcgg                                                                                              <  1:260529/140‑1 (MQ=255)                                        tctcCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAggcggc                                                                                             >  1:730847/1‑140 (MQ=255)                                         ctcCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCa                                                                                            <  2:730847/140‑1 (MQ=255)                                                  aCCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCa                                                                                   >  1:42482/1‑140 (MQ=255)                                                  aCCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCa                                                                                   >  1:88619/1‑140 (MQ=255)                                                  aCCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCa                                                                                   >  1:819324/1‑140 (MQ=255)                                                   ccATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCa                                                                                                   >  2:712756/1‑123 (MQ=255)                                                   ccATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCa                                                                                                   <  1:712756/123‑1 (MQ=255)                                                          gATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCg                                                                           <  1:939964/140‑1 (MQ=255)                                                          gATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCg                                                                           <  2:242505/140‑1 (MQ=255)                                                            tGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATATAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGc                                                                             <  2:299877/136‑1 (MQ=255)                                                            tGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATATAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGc                                                                             >  1:299877/1‑136 (MQ=255)                                                            tGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGAt                                                                         >  2:213446/1‑140 (MQ=255)                                                            tGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGAt                                                                         >  2:368082/1‑140 (MQ=255)                                                              cAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGAttt                                                                       <  2:42482/140‑1 (MQ=255)                                                                ggTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGc                                                                             >  1:921541/1‑132 (MQ=255)                                                                ggTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGc                                                                             <  2:921541/132‑1 (MQ=255)                                                                ggTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATttca                                                                     >  2:416522/1‑140 (MQ=255)                                                                    aGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCg                                                                              <  1:340104/127‑1 (MQ=255)                                                                    aGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCg                                                                              >  2:340104/1‑127 (MQ=255)                                                                    aGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATttcattca                                                                 <  1:906291/140‑1 (MQ=255)                                                                    aGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATttcattca                                                                 <  2:305278/140‑1 (MQ=255)                                                                    aGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATttcattca                                                                 <  1:416522/140‑1 (MQ=255)                                                                     gCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTtcattcat                                                                >  1:267639/1‑140 (MQ=255)                                                                     gCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTtcattcat                                                                >  2:935060/1‑140 (MQ=255)                                                                     gCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTtcagtcat                                                                >  2:919122/1‑140 (MQ=255)                                                                       cGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATgg                                                              <  2:819324/140‑1 (MQ=255)                                                                         ggTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGgtg                                                            <  2:992288/140‑1 (MQ=255)                                                                               cgtcgtAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGccc                                                      >  1:757924/1‑140 (MQ=255)                                                                                   gtAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGt                                                  <  1:449220/140‑1 (MQ=255)                                                                                    tAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGtt                                                 >  2:139536/1‑140 (MQ=255)                                                                                      aTAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCg                                               <  2:757924/140‑1 (MQ=255)                                                                                       tAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGc                                              <  2:88619/140‑1 (MQ=255)                                                                                       tAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGc                                              <  1:935060/140‑1 (MQ=255)                                                                                        aGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGcc                                             >  1:615267/1‑140 (MQ=255)                                                                                            aaGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGt                                         >  1:811083/1‑140 (MQ=255)                                                                                                 aGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAgg                                    <  2:811083/140‑1 (MQ=255)                                                                                                   aaGTTATACACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGt                                  >  1:94812/1‑140 (MQ=255)                                                                                                        ataCACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGt                             >  1:890469/1‑140 (MQ=255)                                                                                                        ataCACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGt                             >  1:531652/1‑140 (MQ=255)                                                                                                         taCACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTc                                 >  2:423996/1‑135 (MQ=255)                                                                                                         taCACCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTc                                 <  1:423996/135‑1 (MQ=255)                                                                                                          acacCGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCg                           <  1:919122/140‑1 (MQ=255)                                                                                                             ccGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCATGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTAGTGa                        >  2:879469/1‑140 (MQ=255)                                                                                                             ccGCGCGGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGa                        <  2:531652/140‑1 (MQ=255)                                                                                                              cgcgcgGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGaa                       >  1:21194/1‑140 (MQ=255)                                                                                                              cgcgcgGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGaa                       >  1:270879/1‑140 (MQ=255)                                                                                                               gcgcgGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGt                             <  2:201128/133‑1 (MQ=255)                                                                                                               gcgcgGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGt                             >  1:201128/1‑133 (MQ=255)                                                                                                               gcgcgGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAAt                      >  1:102929/1‑140 (MQ=255)                                                                                                                cgcgGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGGCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATg                     >  2:687063/1‑140 (MQ=255)                                                                                                                cgcgGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATg                     >  2:719355/1‑140 (MQ=255)                                                                                                                cgcgGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATg                     >  2:281572/1‑140 (MQ=255)                                                                                                                cgcgGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATg                     <  1:585714/140‑1 (MQ=255)                                                                                                                cgcgGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATg                     >  1:581271/1‑140 (MQ=255)                                                                                                                cgcgGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATg                     <  1:479218/140‑1 (MQ=255)                                                                                                                cgcgGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATACATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATg                     >  1:527520/1‑140 (MQ=255)                                                                                                                  cgGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTa                   <  2:21194/140‑1 (MQ=255)                                                                                                                  cgGGCGTTGTGGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTAGTCGTGAATGTa                   <  2:94812/140‑1 (MQ=255)                                                                                                                          gtgGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATgg           <  2:102929/140‑1 (MQ=255)                                                                                                                          gtgGCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATgg           <  1:719355/140‑1 (MQ=255)                                                                                                                             gCTGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTAt        <  1:139536/140‑1 (MQ=255)                                                                                                                               tGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATcg      <  2:581271/140‑1 (MQ=255)                                                                                                                               tGACAGAGCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATACATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATcg      <  2:527520/140‑1 (MQ=255)                                                                                                                                   agagCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGt  <  1:413653/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         GGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGT  >  NC_000913/4305214‑4305483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑