Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,421,823	T→C	V39A (GTA→GCA)	ulaC →	L‑ascorbate specific PTS enzyme IIA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,421,823	0	T	C	100.0%	251.7 / NA	71	V39A (GTA→GCA)	ulaC	L‑ascorbate specific PTS enzyme IIA component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/37);  total (34/37)

GTGAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGT  >  NC_000913/4421690‑4421955                                                                                                                                      |                                                                                                                                     gtgAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGcc                                                                                                                                <  2:3631/140‑1 (MQ=255) gtgAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGcc                                                                                                                                >  2:365962/1‑140 (MQ=255) gtgAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGcc                                                                                                                                >  1:61767/1‑140 (MQ=255) gtgAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGcc                                                                                                                                >  2:579962/1‑140 (MQ=255)  tgAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCcg                                                                                                                               <  2:279701/140‑1 (MQ=255)        aaGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTtac                                                                                                                         >  1:408477/1‑140 (MQ=255)         aGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATCGCAGGAAGCGGTGAAAATCGCCGTTGACCTGTTGGTGGCGGCAGAGGTGGCAGAGCCGCGTtact                                                                                                                        >  2:373219/1‑140 (MQ=255)          ggACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAGTCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTtacta                                                                                                                       >  1:951064/1‑140 (MQ=255)          ggACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTtacta                                                                                                                       <  2:758997/140‑1 (MQ=255)            aCGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAACAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTAcc                                                                                                                     >  2:236484/1‑140 (MQ=255)             cgcgCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCa                                                                                                                    <  2:273896/140‑1 (MQ=255)              gcgcCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAg                                                                                                                   >  2:303092/1‑140 (MQ=255)              gcgcCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAg                                                                                                                   >  2:348600/1‑140 (MQ=255)              gcgcCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAg                                                                                                                   >  2:894555/1‑140 (MQ=255)              gcgcCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAAGCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAg                                                                                                                   >  2:991360/1‑140 (MQ=255)               cgcCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAgg                                                                                                                  >  2:967912/1‑140 (MQ=255)               cgcCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAgg                                                                                                                  <  1:894555/140‑1 (MQ=255)               cgcCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAgg                                                                                                                  <  1:613040/140‑1 (MQ=255)               cgcCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAgg                                                                                                                  >  2:183360/1‑140 (MQ=255)                gcCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGc                                                                                                                 >  2:5031/1‑140 (MQ=255)                gcCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGc                                                                                                                 >  2:868480/1‑140 (MQ=255)                 ccATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTtacta                                                                                                                       >  1:576680/1‑133 (MQ=255)                 ccATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTtacta                                                                                                                       <  2:576680/133‑1 (MQ=255)                 ccATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCg                                                                                                                >  1:415616/1‑140 (MQ=255)                 ccATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCg                                                                                                                <  1:579962/140‑1 (MQ=255)                 ccATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCg                                                                                                                >  2:199947/1‑140 (MQ=255)                 ccATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGGGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCg                                                                                                                >  2:109834/1‑140 (MQ=255)                     gAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTc                                                                                                            >  2:656602/1‑140 (MQ=255)                         ttACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCATGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGa                                                                                                        <  1:957433/140‑1 (MQ=255)                         ttACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGa                                                                                                        <  1:868480/140‑1 (MQ=255)                         ttACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGa                                                                                                        <  1:885152/140‑1 (MQ=255)                         ttACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGa                                                                                                        <  2:229066/140‑1 (MQ=255)                              tGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCg                                                                                                   <  1:925520/140‑1 (MQ=255)                              tGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCg                                                                                                   <  2:492634/140‑1 (MQ=255)                              tGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAACAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCg                                                                                                   <  1:236484/140‑1 (MQ=255)                                aTTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGtt                                                                                                 >  2:119698/1‑140 (MQ=255)                                 ttCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTg                                                                                                >  2:683114/1‑140 (MQ=255)                                   cGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAGTCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGaa                                                                                              <  2:951064/140‑1 (MQ=255)                                       ggCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGt                                                                                          <  1:656602/140‑1 (MQ=255)                                        gCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGtt                                                                                         <  1:683114/140‑1 (MQ=255)                                        gCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGtt                                                                                         <  1:183360/140‑1 (MQ=255)                                         cGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTc                                                                                        <  2:683813/140‑1 (MQ=255)                                            aaaaTAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGt                                                                                     <  1:119698/140‑1 (MQ=255)                                             aaataaatCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTc                                                                                    <  1:199947/140‑1 (MQ=255)                                                          cGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTAt                                                                       <  1:348600/140‑1 (MQ=255)                                                                cAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTcc                                                                 >  1:152266/1‑140 (MQ=255)                                                                 aGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCg                                                                <  1:991360/140‑1 (MQ=255)                                                                             agaCATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATg                                                    >  1:964789/1‑140 (MQ=255)                                                                                 aTGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGc                                                >  1:214509/1‑140 (MQ=255)                                                                                 aTGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGc                                                >  1:443115/1‑140 (MQ=255)                                                                                  tGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCa                                               <  1:5031/140‑1 (MQ=255)                                                                                     cAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACgg                                            <  2:408477/140‑1 (MQ=255)                                                                                     cAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACgg                                            <  2:443115/140‑1 (MQ=255)                                                                                       ggAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGc                                          >  1:463681/1‑140 (MQ=255)                                                                                       ggAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGc                                          <  2:415616/140‑1 (MQ=255)                                                                                         aaGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGt                                        <  1:609720/140‑1 (MQ=255)                                                                                         aaGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGt                                        >  2:58833/1‑140 (MQ=255)                                                                                         aaGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGt                                        >  2:185097/1‑140 (MQ=255)                                                                                           gCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACgg                                            <  1:285800/134‑1 (MQ=255)                                                                                           gCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACgg                                            >  2:285800/1‑134 (MQ=255)                                                                                           gCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTcc                                      <  2:463681/140‑1 (MQ=255)                                                                                           gCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTcc                                      >  1:519290/1‑140 (MQ=255)                                                                                                 aaaaTCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAaga                                <  2:524380/140‑1 (MQ=255)                                                                                                  aaaTCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAagag                               >  1:651180/1‑140 (MQ=255)                                                                                                      cGGCGTTGACCTGCTGGTGGCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCg                           >  2:347008/1‑140 (MQ=255)                                                                                                                    tggtggCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTTGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGt             <  2:61767/140‑1 (MQ=255)                                                                                                                     ggtggCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGtt            <  1:58833/140‑1 (MQ=255)                                                                                                                      gtggCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGttt           <  2:8889/140‑1 (MQ=255)                                                                                                                      gtggCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGttt           <  1:365962/140‑1 (MQ=255)                                                                                                                      gtggCGGCAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCC‑‑‑‑TTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTtctct       >  2:157967/1‑140 (MQ=255)                                                                                                                        ggcggcAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTtct         >  1:895516/1‑140 (MQ=255)                                                                                                                         gcggcAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTtctc        >  1:307516/1‑140 (MQ=255)                                                                                                                             cAGATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTg    >  1:740840/1‑140 (MQ=255)                                                                                                                               gATGTGGCAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGt  <  2:740840/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     GTGAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGT  >  NC_000913/4421690‑4421955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑