Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,451,008	A→G	intergenic (+90/‑50)	ytfQ → / → ytfR	galactofuranose ABC transporter periplasmic binding protein/galactofuranose ABC transporter putative ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,451,008	0	A	G	100.0%	197.8 / NA	56	intergenic (+90/‑50)	ytfQ/ytfR	galactofuranose ABC transporter periplasmic binding protein/galactofuranose ABC transporter putative ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/31);  total (25/31)

TACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGT  >  NC_000913/4450873‑4451140                                                                                                                                        |                                                                                                                                     tACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCcgc                                                                                                                                  <  2:705108/140‑1 (MQ=255)  aCCGCAAAAGAAGAATCAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCcgcg                                                                                                                                 >  1:954574/1‑140 (MQ=255)      cAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCtt                                                                                                                             >  1:283705/1‑140 (MQ=255)      cAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCtt                                                                                                                             >  2:96561/1‑140 (MQ=255)      cAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCtt                                                                                                                             >  2:43538/1‑140 (MQ=255)       aaaaGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                            <  1:96561/140‑1 (MQ=255)        aaaGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                           <  2:621776/140‑1 (MQ=255)          agaagaATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                         <  1:331287/140‑1 (MQ=255)             agaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGc                                                                                                                      <  2:283705/140‑1 (MQ=255)                 ttAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTAc                                                                                                                  <  1:804564/140‑1 (MQ=255)                     agaagaagaAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTggg                                                                                                              >  1:1000624/1‑140 (MQ=255)                     agaagaagaAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTggg                                                                                                              >  1:659621/1‑140 (MQ=255)                        agaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGc                                                                                                           <  1:43538/140‑1 (MQ=255)                         gaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACt                                                                                                                 <  1:102559/133‑1 (MQ=255)                         gaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACt                                                                                                                 >  2:102559/1‑133 (MQ=255)                         gaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCa                                                                                                          <  2:659621/140‑1 (MQ=255)                         gaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCa                                                                                                          >  2:979632/1‑140 (MQ=255)                         gaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCa                                                                                                          <  1:273881/140‑1 (MQ=255)                                      gggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCgg                                                                                             >  1:227742/1‑140 (MQ=255)                                          aTTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGa                                                                                         >  1:603516/1‑140 (MQ=255)                                            tGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGaaa                                                                                       >  2:165961/1‑140 (MQ=255)                                                   tGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATgac                                                                                >  1:717207/1‑140 (MQ=255)                                                     cTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATgacga                                                                              <  1:353582/140‑1 (MQ=255)                                                     cTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATgacga                                                                              >  2:317868/1‑140 (MQ=255)                                                     cTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATgacga                                                                              <  2:1000624/140‑1 (MQ=255)                                                      tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATgacgac                                                                             <  2:869021/140‑1 (MQ=255)                                                      tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATgacgac                                                                             <  1:829540/140‑1 (MQ=255)                                                      tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATgacgac                                                                             <  2:227742/140‑1 (MQ=255)                                                         gCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGAcgaccga                                                                          <  1:645691/140‑1 (MQ=255)                                                           cTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACgaccgacc                                                                        <  1:472545/140‑1 (MQ=255)                                                             gATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCaa                                                                      >  2:990351/1‑140 (MQ=255)                                                              aTGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAac                                                                     >  2:989874/1‑140 (MQ=255)                                                               tGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAaca                                                                    <  1:355697/140‑1 (MQ=255)                                                               tGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAaca                                                                    >  2:649870/1‑140 (MQ=255)                                                                    aTTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAgg                                                               <  1:317868/140‑1 (MQ=255)                                                                    aTTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAgg                                                               >  1:775082/1‑140 (MQ=255)                                                                              cGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCg                                                     <  2:787862/140‑1 (MQ=255)                                                                              cGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCg                                                     <  1:649870/140‑1 (MQ=255)                                                                                gATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCGGGAGATCCTCCGCa                                                   >  2:421371/1‑140 (MQ=255)                                                                                   aaGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCg                                                <  1:165961/140‑1 (MQ=255)                                                                                                         cGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTccc                          <  2:136321/140‑1 (MQ=255)                                                                                                         cGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTccc                          <  2:225720/140‑1 (MQ=255)                                                                                                         cGATGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTccc                          <  1:979632/140‑1 (MQ=255)                                                                                                            tGGTGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCgg                       >  1:355959/1‑140 (MQ=255)                                                                                                               tGCACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGt                    >  1:850797/1‑140 (MQ=255)                                                                                                                 cACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCa                  <  1:990351/140‑1 (MQ=255)                                                                                                                 cACTGAAGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCa                  <  2:24485/140‑1 (MQ=255)                                                                                                                      aaGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCg             >  1:304053/1‑140 (MQ=255)                                                                                                                         ccTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCg                        <  1:924436/126‑1 (MQ=255)                                                                                                                         ccTGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCg                        >  2:924436/1‑126 (MQ=255)                                                                                                                           tGATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGa        <  2:717207/140‑1 (MQ=255)                                                                                                                            gATGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGAc       >  2:548125/1‑140 (MQ=255)                                                                                                                             aTGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACa      >  2:476355/1‑140 (MQ=255)                                                                                                                             aTGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACa      <  1:989874/140‑1 (MQ=255)                                                                                                                              tGCGACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCAGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACaa     >  2:206408/1‑140 (MQ=255)                                                                                                                                 gACGCTTGCCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGt  <  2:214104/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     TACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGT  >  NC_000913/4450873‑4451140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑