Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,523,881	C→T	G72S (GGC→AGC)	yjhG ←	KpLE2 phage‑like element; D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,523,881	0	C	T	100.0%	235.3 / NA	67	G72S (GGC→AGC)	yjhG	KpLE2 phage‑like element; D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/41);  total (26/41)

TCGGAGACGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAG  >  NC_000913/4523745‑4524013                                                                                                                                         |                                                                                                                                     atcgggaCGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTAcc                                                                                                                                   <  2:810216/135‑1 (MQ=255)  cGGAGACGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCg                                                                                                                                  >  1:534504/1‑140 (MQ=255)  cGGAGACGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCg                                                                                                                                  >  1:1000126/1‑140 (MQ=255)   ggAGACGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGa                                                                                                                                 <  1:605342/140‑1 (MQ=255)   ggAGACGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGa                                                                                                                                 <  2:812000/140‑1 (MQ=255)        cGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTa                                                                                                                            <  1:491191/140‑1 (MQ=255)         gTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGa                                                                                                                                 >  2:316877/1‑134 (MQ=255)         gTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGa                                                                                                                                 <  1:316877/134‑1 (MQ=255)          tACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACt                                                                                                                          >  1:276261/1‑140 (MQ=255)          tACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACt                                                                                                                          <  1:499455/140‑1 (MQ=255)           acacGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTg                                                                                                                         <  2:930535/140‑1 (MQ=255)             acGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAg                                                                                                                       <  1:249459/140‑1 (MQ=255)             acGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAg                                                                                                                       <  2:276261/140‑1 (MQ=255)             acGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAg                                                                                                                       <  1:336098/140‑1 (MQ=255)             acGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAg                                                                                                                       <  1:713334/140‑1 (MQ=255)                 cATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTg                                                                                                                         <  1:779681/134‑1 (MQ=255)                 cATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTg                                                                                                                         >  2:779681/1‑134 (MQ=255)                 cATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGt                                                                                                                   >  1:810931/1‑140 (MQ=255)                 cATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGt                                                                                                                   >  1:112198/1‑140 (MQ=255)                  aTAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTa                                                                                                                  <  1:639765/140‑1 (MQ=255)                   tAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGCACTGAGCAGTaa                                                                                                                 >  1:625239/1‑140 (MQ=255)                    agggcagggcATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTaaa                                                                                                                >  1:830436/1‑140 (MQ=255)                      ggcagggcaTGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAAt                                                                                                              >  2:724067/1‑140 (MQ=255)                           ggcaTGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGt                                                                                                         >  1:218023/1‑140 (MQ=255)                           ggcaTGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGt                                                                                                         >  2:860145/1‑140 (MQ=255)                             caTGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTcc                                                                                                       <  1:466414/140‑1 (MQ=255)                              aTGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTccc                                                                                                      <  2:830436/140‑1 (MQ=255)                                ggTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGa                                                                                                    >  2:361484/1‑140 (MQ=255)                                ggTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTACAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGa                                                                                                    <  2:926739/140‑1 (MQ=255)                                 gTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGAt                                                                                                   <  2:1000126/140‑1 (MQ=255)                                     gCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAg                                                                                               >  2:333702/1‑140 (MQ=255)                                       tttAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCt                                                                                             <  2:495263/140‑1 (MQ=255)                                          aataaCCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGt                                                                                          >  2:935722/1‑140 (MQ=255)                                                  cGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGAc                                                                                  <  1:333702/140‑1 (MQ=255)                                                    gccgccGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCAGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCa                                                                                >  2:179770/1‑140 (MQ=255)                                                       gccgcTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAAcc                                                                             <  1:177952/140‑1 (MQ=255)                                                         cgcTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCa                                                                           <  1:724067/140‑1 (MQ=255)                                                          gcTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCAGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCAt                                                                          <  1:179770/140‑1 (MQ=255)                                                           cTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCAtt                                                                         <  2:228610/140‑1 (MQ=255)                                                              tCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCg                                                                      <  2:832351/140‑1 (MQ=255)                                                              tCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCg                                                                      <  1:680408/140‑1 (MQ=255)                                                              tCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCg                                                                      <  2:218023/140‑1 (MQ=255)                                                               cATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCgg                                                                     <  1:361484/140‑1 (MQ=255)                                                                   tGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCAtt                                                                 <  1:278470/140‑1 (MQ=255)                                                                   tGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCAtt                                                                 <  1:763944/140‑1 (MQ=255)                                                                   tGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCAtt                                                                 <  1:903159/140‑1 (MQ=255)                                                                   tGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCAtt                                                                 <  2:165333/140‑1 (MQ=255)                                                                        gTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATgg                                                            <  1:887086/140‑1 (MQ=255)                                                                        gTCCAGTTCGTAATGCCCATGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATgg                                                            <  1:412799/140‑1 (MQ=255)                                                                          ccAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTc                                                          >  2:505649/1‑140 (MQ=255)                                                                               tCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATAcc                                                     >  1:683273/1‑140 (MQ=255)                                                                                            ggTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCg                                        >  2:547013/1‑140 (MQ=255)                                                                                            ggTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCg                                        >  1:997954/1‑140 (MQ=255)                                                                                            ggTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCAAACAG‑TCCCCGc                                       >  2:603284/1‑140 (MQ=255)                                                                                                 aaCGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCgg                                   >  1:83966/1‑140 (MQ=255)                                                                                                  aCGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACGGATCCCCGCTCggg                                  <  2:810931/140‑1 (MQ=255)                                                                                                  aCGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCggg                                  <  1:935722/140‑1 (MQ=255)                                                                                                  aCGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCggg                                  >  2:44347/1‑140 (MQ=255)                                                                                                   cGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGc                                 <  2:462404/140‑1 (MQ=255)                                                                                                   cGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATACCCGCTCGGGc                                 <  2:625239/140‑1 (MQ=255)                                                                                                     ccAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCAAACAG‑TCCCCGCTCGGGCGGt                              <  1:603284/140‑1 (MQ=255)                                                                                                           ccACGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATa                         >  2:695022/1‑140 (MQ=255)                                                                                                             aCGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAg                       <  2:534504/140‑1 (MQ=255)                                                                                                              cGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCgg                               >  1:955850/1‑131 (MQ=255)                                                                                                              cGGGTTTACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCgg                               <  2:955850/131‑1 (MQ=255)                                                                                                                    tACCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATctct                <  1:868590/140‑1 (MQ=255)                                                                                                                     aCCGTCTGCGCCGCGTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTg               <  1:634059/140‑1 (MQ=255)                                                                                                                                  cgTAAGCTACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAg  >  2:818559/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     TCGGAGACGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAG  >  NC_000913/4523745‑4524013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑