Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,573,154	G→A	T247T (ACG→ACA)	yjiT →	putative uncharacterized protein YjiT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,573,154	0	G	A	100.0%	218.6 / NA	62	T247T (ACG→ACA)	yjiT	putative uncharacterized protein YjiT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/31);  total (31/31)

ATATGGCTGATAACCTCAATTCCTTAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGG  >  NC_000913/4573018‑4573290                                                                                                                                         |                                                                                                                                         atatGGCTGATAACCTCAATTCCTTAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACAt                                                                                                                                       >  2:651259/1‑140 (MQ=255) atatGGCTGATAACCTCAATTCCTTAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACAt                                                                                                                                       >  2:260404/1‑140 (MQ=255)      gCTGATAACCTCAATTCCTTAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACAtttttt                                                                                                                                  <  2:419353/140‑1 (MQ=255)      gCTGATAACCTCAATTCCTTAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACAtttttt                                                                                                                                  <  1:660585/140‑1 (MQ=255)         gATAACCTCAATTCCTTAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGaa                                                                                                                               >  2:779544/1‑140 (MQ=255)         gATAACCTCAATTCCTTAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGaa                                                                                                                               >  2:313252/1‑140 (MQ=255)         gATAACCTCAATTCCTTAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGaa                                                                                                                               >  1:846634/1‑140 (MQ=255)         gATAACCTCAATTCCTTAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGaa                                                                                                                               >  1:530214/1‑140 (MQ=255)                 cAATTCCTTAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGc                                                                                                                       <  1:175401/140‑1 (MQ=255)                 cAATTCCTTAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGc                                                                                                                       <  2:62839/140‑1 (MQ=255)                      ccTTAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATgc                                                                                                                  <  2:846634/140‑1 (MQ=255)                        ttAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATgcgc                                                                                                                <  1:779544/140‑1 (MQ=255)                         tAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCa                                                                                                               <  2:995395/140‑1 (MQ=255)                                 actCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGt                                                                                                       >  1:485236/1‑140 (MQ=255)                                 actCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGt                                                                                                       >  1:823493/1‑140 (MQ=255)                                  ctCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTa                                                                                                      <  1:313252/140‑1 (MQ=255)                                  ctCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTa                                                                                                      <  1:924160/140‑1 (MQ=255)                                      caACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAgg                                                                                                  >  1:288009/1‑140 (MQ=255)                                                 aTCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTa                                                                                                      >  2:915054/1‑125 (MQ=255)                                                 aTCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTa                                                                                                      <  1:915054/125‑1 (MQ=255)                                                   cacaAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTa                                                                                                      >  2:657396/1‑123 (MQ=255)                                                   cacaAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTa                                                                                                      <  1:657396/123‑1 (MQ=255)                                                   cacaAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACt                                                                                     >  1:159908/1‑140 (MQ=255)                                                   cacaAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACt                                                                                     >  2:926656/1‑140 (MQ=255)                                                    acaAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTa                                                                                    >  2:625906/1‑140 (MQ=255)                                                     caAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTAc                                                                                   <  2:31617/140‑1 (MQ=255)                                                     caAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTAc                                                                                   <  2:159908/140‑1 (MQ=255)                                                            ccAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACaaaaaaa                                                                            <  2:20772/140‑1 (MQ=255)                                                                    acaGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTa                                                                    >  1:596635/1‑140 (MQ=255)                                                                          tGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAAt                                                                           <  1:175777/127‑1 (MQ=255)                                                                          tGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAAt                                                                           >  2:175777/1‑127 (MQ=255)                                                                          tGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGtt                                                              >  1:40172/1‑140 (MQ=255)                                                                          tGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGtt                                                              >  2:188194/1‑140 (MQ=255)                                                                                    cACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTa                                                    <  2:823493/140‑1 (MQ=255)                                                                                      cccTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTAtt                                                  <  1:625906/140‑1 (MQ=255)                                                                                         tACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGt                                               <  1:260404/140‑1 (MQ=255)                                                                                           ccTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTg                                                 <  1:843219/136‑1 (MQ=255)                                                                                           ccTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTg                                                 >  2:843219/1‑136 (MQ=255)                                                                                                     cGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATc                                   <  1:716351/140‑1 (MQ=255)                                                                                                     cGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATc                                   >  2:48372/1‑140 (MQ=255)                                                                                                     cGAGTTTCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATc                                   >  1:431420/1‑140 (MQ=255)                                                                                                         tttCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGATTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGa                               <  2:114777/140‑1 (MQ=255)                                                                                                         tttCCAATACCGTTAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGa                               <  2:40172/140‑1 (MQ=255)                                                                                                                     ttAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAgggg                   >  2:542933/1‑139 (MQ=255)                                                                                                                     ttAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGt                   <  1:926656/140‑1 (MQ=255)                                                                                                                     ttAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGt                   >  2:105996/1‑140 (MQ=255)                                                                                                                     ttAGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAG‑AAAGCATCCAGATGAGCTTAGGGTg                  >  2:216569/1‑140 (MQ=255)                                                                                                                       aGATGATGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGa                 >  2:123857/1‑140 (MQ=255)                                                                                                                       aGATGATGAAACAGGAACACATTTTTT‑AATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGAt                <  2:661857/140‑1 (MQ=255)                                                                                                                        gatgatGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGAt                <  1:651259/140‑1 (MQ=255)                                                                                                                        gatgatGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGAt                <  2:530214/140‑1 (MQ=255)                                                                                                                        gatgatGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGAt                <  1:48372/140‑1 (MQ=255)                                                                                                                        gatgatGAAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGAt                <  1:188194/140‑1 (MQ=255)                                                                                                                           gatgaAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCt                         <  1:497532/128‑1 (MQ=255)                                                                                                                           gatgaAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCt                         >  2:497532/1‑128 (MQ=255)                                                                                                                             tgaAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTa                       <  1:632938/128‑1 (MQ=255)                                                                                                                             tgaAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTa                       >  2:632938/1‑128 (MQ=255)                                                                                                                             tgaAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTAt           >  2:234924/1‑140 (MQ=255)                                                                                                                             tgaAACAGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTAt           >  2:389911/1‑140 (MQ=255)                                                                                                                                   aGGAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTc     >  1:546081/1‑140 (MQ=255)                                                                                                                                    ggAACACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTcc    >  1:368346/1‑140 (MQ=255)                                                                                                                                      aaCACATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCgg  <  1:234924/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         ATATGGCTGATAACCTCAATTCCTTAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGG  >  NC_000913/4573018‑4573290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑