Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	695,690	A→G	N197S (AAC→AGC)	ubiF →	2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	695,690	0	A	G	100.0%	116.1 / NA	34	N197S (AAC→AGC)	ubiF	2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/18);  total (16/18)

GTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCAAATTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCA  >  NC_000913/695558‑695813                                                                                                                                     |                                                                                                                            gTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCAAATTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATccc                                                                                                                      >  2:617303/1‑140 (MQ=255) gTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCAAATTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATccc                                                                                                                      >  2:103706/1‑140 (MQ=255)            cgcgcgAAGCTGGTGATTGGTGCCGACGGCGCAAATTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCAc                                                                                                           >  1:197827/1‑140 (MQ=255)              cgcgAAGCTGGTGATTGGTGCCGACGGCGCAAATTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCt                                                                                                         >  1:117300/1‑140 (MQ=255)              cgcgAAGCTGGTGATTGGTGCCGACGGCGCAAACTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCt                                                                                                         >  2:163341/1‑140 (MQ=255)                cgAAGCTGGCGATTGGTGCCGACGGCGCAAATTCGCAGGTGCGACAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGATAGCGATTCCGGCGACAGCACCTgg                                                                                                       >  2:889529/1‑140 (MQ=255)                  aaGCTGGTGATTGGTGCCGACGGCGCAAATTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGgca                                                                                                     <  2:164211/140‑1 (MQ=255)                  aaGCTGGTGATTGGTGCCGACGGCGCAAATTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGgca                                                                                                     <  1:617303/140‑1 (MQ=255)                         tGATTGGTGCCGACGGCGCAAATTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAAttt                                                                                              <  1:947856/140‑1 (MQ=255)                           aTTGGTGCCGACGGCGCAAATTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTAc                                                                                            <  1:103706/140‑1 (MQ=255)                               gTGCCGACGGCGCAAATTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCcg                                                                                        >  2:988555/1‑140 (MQ=255)                               gTGCCGACGGCGCAAATTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCcg                                                                                        >  2:59143/1‑140 (MQ=255)                                  ccGACGGCGCAAATTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCcggac                                                                                     >  2:309738/1‑140 (MQ=255)                                  ccGACGGCGCAAATTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCcggac                                                                                     >  2:245429/1‑140 (MQ=255)                                    gACGGCGCAAATTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCcggacgg                                                                                   <  1:880629/140‑1 (MQ=255)                                         cgcAAATTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACcgc                                                                              <  1:106414/140‑1 (MQ=255)                                                                     ggAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAAATTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTg                                                  <  1:542355/140‑1 (MQ=255)                                                                             cGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGc                                          <  1:245429/140‑1 (MQ=255)                                                                                 catgcatgGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGgt                                      >  2:800757/1‑140 (MQ=255)                                                                                  atgcatgGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGATAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGgtg                                     <  1:889529/140‑1 (MQ=255)                                                                                  atgcatgGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCTCTGgtg                                     <  1:537305/140‑1 (MQ=255)                                                                                    gcatgGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGtgtg                                   <  1:988555/140‑1 (MQ=255)                                                                                       tgGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTa                                >  2:490543/1‑140 (MQ=255)                                                                                       tgGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTa                                <  2:774680/140‑1 (MQ=255)                                                                                       tgGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTa                                <  1:490543/140‑1 (MQ=255)                                                                                        ggCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCAGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCg                                           >  1:597282/1‑128 (MQ=255)                                                                                        ggCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCAGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCg                                           <  2:597282/128‑1 (MQ=255)                                                                                              aTGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTATGActct                         >  1:447322/1‑140 (MQ=255)                                                                                                    aGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTATGACTCTCCGgcg                   >  1:697382/1‑140 (MQ=255)                                                                                                       cGTGCATGTTGATTAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGt                >  2:761537/1‑140 (MQ=255)                                                                                                                   ttAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTg    >  1:749405/1‑140 (MQ=255)                                                                                                                   ttAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTg    <  1:163341/140‑1 (MQ=255)                                                                                                                    tAGCGTCCAGTGCGCGAGCGATCCTGTCGCAAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGc   <  2:697382/140‑1 (MQ=255)                                                                                                                    tAGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGc   <  2:117300/140‑1 (MQ=255)                                                                                                                     aGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCa  <  2:447322/140‑1 (MQ=255)                                                                                                                     aGCGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCa  <  2:197827/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                            GTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCAAATTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCA  >  NC_000913/695558‑695813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑