Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	758,773	T→C	I23T (ATA→ACA)	sucA →	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	758,773	0	T	C	100.0%	144.3 / NA	41	I23T (ATA→ACA)	sucA	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/19);  total (22/19)

CACATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTT  >  NC_000913/758641‑758902                                                                                                                                     |                                                                                                                                  cacaTCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAg                                                                                                                            >  2:239037/1‑140 (MQ=255) cacaTCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCAT‑AAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGc                                                                                                                           <  1:575448/140‑1 (MQ=255)          gtgCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGaa                                                                                                                   <  2:37399/140‑1 (MQ=255)          gtgCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGaa                                                                                                                   <  1:239037/140‑1 (MQ=255)          gtgCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGaa                                                                                                                   >  1:37399/1‑140 (MQ=255)            gCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGaa                                                                                                                               >  2:929606/1‑126 (MQ=255)            gCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGaa                                                                                                                               <  1:929606/126‑1 (MQ=255)            gCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGa                                                                                                                 >  1:860711/1‑140 (MQ=255)              gTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGAct                                                                                                               >  1:318562/1‑140 (MQ=255)                ggTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAGAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGActtc                                                                                                             >  2:176477/1‑140 (MQ=255)                     taTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAGAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAAc                                                                                                        <  1:176477/140‑1 (MQ=255)                              cGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGa                                                                                               >  1:309126/1‑140 (MQ=255)                               gAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGAc                                                                                              <  1:583322/140‑1 (MQ=255)                                   tAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCgg                                                                                          >  1:481280/1‑140 (MQ=255)                                      gCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTg                                                                                       <  1:628602/140‑1 (MQ=255)                                       cATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGa                                                                                      <  2:481280/140‑1 (MQ=255)                                                     ttAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGtt                                                                        <  2:309126/140‑1 (MQ=255)                                                      tAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGGTCCTGACTCGGTTGACGCTAACTGGCGTTc                                                                       >  1:797529/1‑140 (MQ=255)                                                      tAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTc                                                                       >  1:685546/1‑140 (MQ=255)                                                        aGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGa                                                                     <  1:568098/140‑1 (MQ=255)                                                                  aTGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCcagca                                                           <  2:318562/140‑1 (MQ=255)                                                                   tGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCcagcag                                                          <  1:147785/140‑1 (MQ=255)                                                                    gCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGt                                                         >  1:41596/1‑140 (MQ=255)                                                                     cAGAACAGCGCTTTGGAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGtt                                                        <  2:175156/140‑1 (MQ=255)                                                                        aaCAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCcag                                                             >  2:210353/1‑132 (MQ=255)                                                                        aaCAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCcag                                                             <  1:210353/132‑1 (MQ=255)                                                                        aaCAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTAcc                                                     <  2:41596/140‑1 (MQ=255)                                                                             cgcTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTa                                                >  2:514434/1‑140 (MQ=255)                                                                               cTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTAc                                               <  1:793751/139‑1 (MQ=255)                                                                               cTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTAc                                               >  2:793751/1‑139 (MQ=255)                                                                                    aaaGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGt                                         <  2:529234/140‑1 (MQ=255)                                                                                              tGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGAt                               >  2:940979/1‑140 (MQ=255)                                                                                                 aCTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCaa                            >  1:744761/1‑140 (MQ=255)                                                                                                     ttcttACCTCTCTGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTcc                        <  2:744761/140‑1 (MQ=255)                                                                                                               tctGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCaaa               <  2:364212/139‑1 (MQ=255)                                                                                                               tctGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCaaa               >  1:364212/1‑139 (MQ=255)                                                                                                               tctGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAAc              >  2:664218/1‑140 (MQ=255)                                                                                                               tctGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAAc              >  1:682935/1‑140 (MQ=255)                                                                                                                 tGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAAcgc            >  1:647990/1‑140 (MQ=255)                                                                                                                 tGGCGCAAACCAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAAcgc            >  2:787919/1‑140 (MQ=255)                                                                                                                           cAGAGCTGGACAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATAttt  >  2:354010/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                  CACATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTT  >  NC_000913/758641‑758902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑