Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	790,924	C→T	A36T (GCG→ACG)	galT ←	galactose‑1‑phosphate uridylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	790,924	0	C	T	100.0%	101.6 / NA	30	A36T (GCG→ACG)	galT	galactose‑1‑phosphate uridylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/13);  total (17/13)

AGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGA  >  NC_000913/790787‑791060                                                                                                                                          |                                                                                                                                         aGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGtcc                                                                                                                                        <  2:91983/140‑1 (MQ=255)             aTTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGgc                                                                                                                            >  1:398021/1‑140 (MQ=255)                                   gtgtAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCATCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAAc                                                                                                      >  2:579895/1‑140 (MQ=255)                                         tCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAAt                                                                                                >  2:831875/1‑140 (MQ=255)                                         tCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAAt                                                                                                <  2:463650/140‑1 (MQ=255)                                                 tttATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGcc                                                                                        <  1:569785/140‑1 (MQ=255)                                                                       ttACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAgcggcg                                                                  <  1:147037/140‑1 (MQ=255)                                                                       ttACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAgcggcg                                                                  <  2:814811/140‑1 (MQ=255)                                                                               gcAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGgat                                                          >  1:141147/1‑140 (MQ=255)                                                                                cAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGgatg                                                         >  2:638222/1‑140 (MQ=255)                                                                                     gAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCaa                                                    >  2:943744/1‑140 (MQ=255)                                                                                     gAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCaa                                                    >  2:941769/1‑140 (MQ=255)                                                                                     gAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCaa                                                    <  2:568909/140‑1 (MQ=255)                                                                                     gAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCaa                                                    <  1:943744/140‑1 (MQ=255)                                                                                         cAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACggg                                                <  2:398021/140‑1 (MQ=255)                                                                                          aaTCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGa                                               <  2:951457/140‑1 (MQ=255)                                                                                            tCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGAtt                                             >  1:501257/1‑140 (MQ=255)                                                                                                gATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAAt                                         <  2:501257/140‑1 (MQ=255)                                                                                                     tGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGt                                    >  1:102691/1‑140 (MQ=255)                                                                                                     tGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGt                                    >  2:850349/1‑140 (MQ=255)                                                                                                     tGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGt                                    >  2:842481/1‑140 (MQ=255)                                                                                                      gcgcAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTc                                   >  1:971597/1‑140 (MQ=255)                                                                                                          aGGTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATgg                               >  2:771550/1‑140 (MQ=255)                                                                                                            gTAACACCTGTTTGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTc                             <  2:771078/140‑1 (MQ=255)                                                                                                                      tttGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAAt                   >  1:526632/1‑140 (MQ=255)                                                                                                                      tttGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAAt                   >  1:724063/1‑140 (MQ=255)                                                                                                                      tttGGCTGGCGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAAt                   <  1:941769/140‑1 (MQ=255)                                                                                                                           ctggcGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGa              >  1:234039/1‑140 (MQ=255)                                                                                                                           ctggcGTTTCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGa              <  2:234039/140‑1 (MQ=255)                                                                                                                                   tCCTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCtg      <  1:290510/140‑1 (MQ=255)                                                                                                                                     cTGCGTCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCtgtg    >  2:383219/1‑140 (MQ=255)                                                                                                                                       gcgtCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGa  <  1:655335/140‑1 (MQ=255)                                                                                                                                       gcgtCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGa  <  1:332992/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         AGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGA  >  NC_000913/790787‑791060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑