Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	885,013	A→G	V73A (GTT→GCT)	ybjH ←	uncharacterized protein YbjH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	885,013	0	A	G	100.0%	171.0 / NA	48	V73A (GTT→GCT)	ybjH	uncharacterized protein YbjH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/19);  total (29/19)

TGGGAAATTCTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAACATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATCAGGAACCACAACCACTTTGTATCCACTCG  >  NC_000913/884883‑885142                                                                                                                                   |                                                                                                                                  tGGGAAATTCTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGAt                                                                                                                          >  1:948683/1‑140 (MQ=255)  gggAAATTCTCACGAAGGGT‑AAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATgc                                                                                                                        <  2:171628/140‑1 (MQ=255)      aaTTCTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATgcggg                                                                                                                     <  2:950419/140‑1 (MQ=255)          ctcACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGt                                                                                                                 <  1:650860/140‑1 (MQ=255)            cACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATgcg                                                                                                                       >  1:188192/1‑132 (MQ=255)            cACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATgcg                                                                                                                       <  2:188192/132‑1 (MQ=255)                             tGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAgg                                                                                              >  1:14050/1‑140 (MQ=255)                              gCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGa                                                                                             >  2:988562/1‑140 (MQ=255)                               ccTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAg                                                                                            >  1:637843/1‑140 (MQ=255)                                cTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGc                                                                                           <  2:671859/140‑1 (MQ=255)                                   aCTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCcac                                                                                        >  2:923566/1‑140 (MQ=255)                                          tgtgCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACg                                                                                 <  1:988562/140‑1 (MQ=255)                                            tgCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGt                                                                               >  2:103717/1‑140 (MQ=255)                                            tgCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGt                                                                               >  1:704732/1‑140 (MQ=255)                                               gATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTg                                                                            >  1:500105/1‑140 (MQ=255)                                                 tCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCg                                                                          >  1:625846/1‑140 (MQ=255)                                                 tCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCg                                                                          >  2:557157/1‑140 (MQ=255)                                                      cATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGcaca                                                                     >  1:58926/1‑140 (MQ=255)                                                      cATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGcaca                                                                     >  1:339206/1‑140 (MQ=255)                                                               aTTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACg                                                            >  2:373950/1‑140 (MQ=255)                                                                       tGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGc                                                    >  1:640665/1‑140 (MQ=255)                                                                       tGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGc                                                    >  2:370670/1‑140 (MQ=255)                                                                       tGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGc                                                    <  2:637843/140‑1 (MQ=255)                                                                         aTGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGAc                                                       <  2:138640/135‑1 (MQ=255)                                                                         aTGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGAc                                                       >  1:138640/1‑135 (MQ=255)                                                                         aTGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCtt                                                  >  2:679262/1‑140 (MQ=255)                                                                           ggTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGGGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGAc                                                       <  2:561003/133‑1 (MQ=255)                                                                           ggTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGGGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGAc                                                       >  1:561003/1‑133 (MQ=255)                                                                               gtcAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGaa                                            <  2:948683/140‑1 (MQ=255)                                                                                      tGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGctgac                                     <  2:330311/140‑1 (MQ=255)                                                                                      tGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGctgac                                     >  2:720437/1‑140 (MQ=255)                                                                                       ggTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTtgtg                                               <  1:944409/129‑1 (MQ=255)                                                                                       ggTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTtgtg                                               >  2:944409/1‑129 (MQ=255)                                                                                           ttCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAAt                                >  2:297562/1‑140 (MQ=255)                                                                                             cGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATCa                              <  1:297562/140‑1 (MQ=255)                                                                                                       cccTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATCAGGaaccacaa                    >  2:310051/1‑140 (MQ=255)                                                                                                        ccTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATCAGGaaccacaac                   <  1:370670/140‑1 (MQ=255)                                                                                                         cTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATCAGGaaccacaacc                  <  1:103717/140‑1 (MQ=255)                                                                                                         cTTCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATCAGGaaccacaacc                  <  2:640665/140‑1 (MQ=255)                                                                                                          ttCACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATCAGGaaccacaacca                 <  2:500105/140‑1 (MQ=255)                                                                                                            cACCTACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATCAGGAACCACAACCACt               >  2:193443/1‑140 (MQ=255)                                                                                                                tACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATCAGGAACCACAACCACTTTGt           <  2:625846/140‑1 (MQ=255)                                                                                                                tACGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATCAGGAACCACAACCACTTTGt           <  1:679262/140‑1 (MQ=255)                                                                                                                 aCGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATCAGGAACCACAACCACTTTGTa          >  1:37221/1‑140 (MQ=255)                                                                                                                  cGCTGGCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATCAGGAACCACAACCACTTTGTAt         >  1:428817/1‑140 (MQ=255)                                                                                                                       gCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATCAGGAACCACAACCACTTTGTATCCACt    >  1:42010/1‑140 (MQ=255)                                                                                                                       gCAAGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATCAGGAACCACAACCACTTTGTATCCACt    >  1:819994/1‑140 (MQ=255)                                                                                                                         aaGATGGCGAGCATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATCAGGAACCACAACCACTTTGTATCCACTCg  >  2:229004/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                  TGGGAAATTCTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAACATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATCAGGAACCACAACCACTTTGTATCCACTCG  >  NC_000913/884883‑885142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑