Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	45,885	C→T	P27S (CCG→TCG)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	45,885	0	C	T	100.0%	156.5 / NA	45	P27S (CCG→TCG)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/22);  total (23/22)

TGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCG  >  NC_000913/45751‑46019                                                                                                                                       |                                                                                                                                       tGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGttt                                                                                                                                   >  1:346181/1‑140 (MQ=255) tGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGttt                                                                                                                                   <  1:682893/140‑1 (MQ=255)    tGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTg                                                                                                                                <  2:777893/140‑1 (MQ=255)      gTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGa                                                                                                                              <  1:553333/140‑1 (MQ=255)        ttGATACGTAACGCCGCACTGACTCTCATTG‑AAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTAGTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATgg                                                                                                                           <  1:839794/140‑1 (MQ=255)               gTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGt                                                                                                                     <  1:389355/140‑1 (MQ=255)                tAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAACCTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTa                                                                                                                    <  1:966151/140‑1 (MQ=255)                 aaCGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTAc                                                                                                                   <  2:569809/140‑1 (MQ=255)                 aaCGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTAc                                                                                                                   <  1:668316/140‑1 (MQ=255)                      cgcACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTa                                                                                                              >  1:103801/1‑140 (MQ=255)                                       cAAAAAACATGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCt                                                                                             >  1:815031/1‑140 (MQ=255)                                         aaaaaCAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTgg                                                                                           >  2:768316/1‑140 (MQ=255)                                                      aCCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGcc                                                                              >  1:520847/1‑140 (MQ=255)                                                      aCCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGcc                                                                              <  1:734937/140‑1 (MQ=255)                                                           gCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGgcgc                                                                         >  2:786256/1‑140 (MQ=255)                                                           gCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGgcg                                                                          >  1:977516/1‑139 (MQ=255)                                                           gCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGgcg                                                                          <  2:977516/139‑1 (MQ=255)                                                             aaCCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTg                                                                       <  1:814534/140‑1 (MQ=255)                                                                 gTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAAc                                                                   >  2:18160/1‑140 (MQ=255)                                                                            ttGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGa                                                                      <  2:503636/126‑1 (MQ=255)                                                                            ttGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGa                                                                      >  1:503636/1‑126 (MQ=255)                                                                            ttGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGAc                                                        >  2:425715/1‑140 (MQ=255)                                                                             tGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACt                                                       >  2:796485/1‑140 (MQ=255)                                                                                     tcAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGc                                               >  2:113965/1‑140 (MQ=255)                                                                                     tcAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGc                                               >  2:976117/1‑140 (MQ=255)                                                                                             tcctcTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTggg                                       >  2:371008/1‑140 (MQ=255)                                                                                               ctctATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGgcg                                     >  2:767309/1‑140 (MQ=255)                                                                                                     tCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGaa                               >  1:12852/1‑140 (MQ=255)                                                                                                       aCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCt                                          <  1:291741/127‑1 (MQ=255)                                                                                                       aCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCt                                          >  2:291741/1‑127 (MQ=255)                                                                                                       aCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACg                             <  2:862496/140‑1 (MQ=255)                                                                                                       aCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACg                             >  2:752477/1‑140 (MQ=255)                                                                                                         cgccgcATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCt                           >  2:722453/1‑140 (MQ=255)                                                                                                           ccgcATTTTGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCg                         <  2:12852/140‑1 (MQ=255)                                                                                                                ttttGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCggg                    <  2:520847/140‑1 (MQ=255)                                                                                                                   tGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTg                 <  1:767309/140‑1 (MQ=255)                                                                                                                   tGCTGTGGGGAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTg                 <  1:796485/140‑1 (MQ=255)                                                                                                                          gggAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGCCGCTGACTGGATTGGCTTGCTGGGCGCGGGAGCGCTCGCCGGGCTGTTCGTTg          <  2:413448/140‑1 (MQ=255)                                                                                                                          gggAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTg          >  2:778622/1‑140 (MQ=255)                                                                                                                           ggAAGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTgg         >  1:110860/1‑140 (MQ=255)                                                                                                                             aaGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGca       <  2:103801/140‑1 (MQ=255)                                                                                                                             aaGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGca       >  1:963373/1‑140 (MQ=255)                                                                                                                             aaGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGca       <  1:425715/140‑1 (MQ=255)                                                                                                                             aaGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGca       <  1:18160/140‑1 (MQ=255)                                                                                                                             aaGCGGTGGTTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGCTCGTTGGca       >  1:646976/1‑140 (MQ=255)                                                                                                                                  gtggtTCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCg  <  2:963373/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       TGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCG  >  NC_000913/45751‑46019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑