Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	902,828	A→G	L54P (CTG→CCG)	artI ←	putative ABC transporter periplasmic binding protein ArtI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	902,828	0	A	G	100.0%	210.3 / NA	59	L54P (CTG→CCG)	artI	putative ABC transporter periplasmic binding protein ArtI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/28);  total (31/28)

AGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACAGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCAAAACGAATGGTTTCGGCAGCTGTGGCGGAAAGACTAAAACCTGCAATT  >  NC_000913/902693‑902962                                                                                                                                        |                                                                                                                                       aGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGgcgc                                                                                                                                    <  1:741696/140‑1 (MQ=255) aGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGgcgc                                                                                                                                    >  2:861220/1‑140 (MQ=255) aGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGgcgc                                                                                                                                    >  2:165695/1‑140 (MQ=255)   cACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCtt                                                                                                                                  >  2:868700/1‑140 (MQ=255)      cTGCTTTTCACGCTCCGGAGTGATCTCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTtgtg                                                                                                                               <  1:459383/140‑1 (MQ=255)      cTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTtgtg                                                                                                                               >  2:872905/1‑140 (MQ=255)             tCACGCTCCGGAGTGATATCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTc                                                                                                                        >  1:898250/1‑140 (MQ=255)                   tCCGGAGTGATATCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTc                                                                                                                  >  2:129391/1‑140 (MQ=255)                    ccGGAGTGATATCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCa                                                                                                                 <  1:185888/140‑1 (MQ=255)                           gATATCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCaa                                                                                                          <  1:165695/140‑1 (MQ=255)                            atatCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCaa                                                                                                                <  2:94598/133‑1 (MQ=255)                            atatCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCaa                                                                                                                >  1:94598/1‑133 (MQ=255)                             tatCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACg                                                                                                        <  1:129391/140‑1 (MQ=255)                               tCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGAt                                                                                                      >  1:330074/1‑140 (MQ=255)                               tCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGAt                                                                                                      >  2:167405/1‑140 (MQ=255)                                 cATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCt                                                                                                    >  1:732855/1‑140 (MQ=255)                                  aTGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTg                                                                                                   <  1:770699/140‑1 (MQ=255)                                   tGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTgg                                                                                                  <  1:861220/140‑1 (MQ=255)                                   tGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTgg                                                                                                  <  2:705329/140‑1 (MQ=255)                                   tGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTgg                                                                                                  <  2:34159/140‑1 (MQ=255)                                    gCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGgt                                                                                                 >  2:328271/1‑140 (MQ=255)                                       cGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTgg                                                                                                  >  1:631328/1‑136 (MQ=255)                                       cGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTgg                                                                                                  <  2:631328/136‑1 (MQ=255)                                         ccATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGttt                                                                                            >  1:430852/1‑140 (MQ=255)                                             cACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCAt                                                                                        >  1:568182/1‑140 (MQ=255)                                                ggCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCaa                                                                                     <  2:898250/140‑1 (MQ=255)                                                 gCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAAt                                                                                    <  2:716710/140‑1 (MQ=255)                                                     cTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGAt                                                                                <  2:709073/140‑1 (MQ=255)                                                      tACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGAtt                                                                               >  1:502226/1‑140 (MQ=255)                                                       aCGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTc                                                                              <  1:868700/140‑1 (MQ=255)                                                       aCGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTc                                                                              <  2:333514/140‑1 (MQ=255)                                                        cgcgACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTCCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCa                                                                             >  1:154793/1‑140 (MQ=255)                                                          cgACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCaaa                                                                           <  2:330074/140‑1 (MQ=255)                                                            aCGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACg                                                                         <  2:568977/140‑1 (MQ=255)                                                            aCGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACg                                                                         <  1:250207/140‑1 (MQ=255)                                                            aCGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACg                                                                         <  2:430852/140‑1 (MQ=255)                                                            aCGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTCCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACg                                                                         <  2:154793/140‑1 (MQ=255)                                                                          ttGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCt                                                           >  2:894577/1‑140 (MQ=255)                                                                           tGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCtt                                                          <  2:568182/140‑1 (MQ=255)                                                                                   ggCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCa                                                  >  1:54550/1‑140 (MQ=255)                                                                                    gCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCaa                                                 >  1:181991/1‑140 (MQ=255)                                                                                        tCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACTGAGGATAGGAGGCTTCGGTAGCAAAACg                                             >  2:999150/1‑140 (MQ=255)                                                                                         cAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCAAAACGa                                            <  2:732855/140‑1 (MQ=255)                                                                                                  ggTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCAAAACGAATGGTTTCg                                   >  2:442367/1‑140 (MQ=255)                                                                                                        agaAAGTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCAAAACGAATGGTTTCGGCAGCt                             >  1:446808/1‑140 (MQ=255)                                                                                                             gTGCAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCAAAACGAATGGTTTCGGCAGCTGTGGc                        >  2:246691/1‑140 (MQ=255)                                                                                                                cAGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCAAAACGAATGGTTTCGGCAGCTGTGGCGGa                     <  1:894577/140‑1 (MQ=255)                                                                                                                 aGGTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCAAAACGAATGGTTTCGGCAGCTGTGGCGGaa                    <  1:442367/140‑1 (MQ=255)                                                                                                                  ggTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCAAAACGAATGGTTTCGGCAGCTGTGGCGGaaa                   >  1:148258/1‑140 (MQ=255)                                                                                                                   gTTGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCAAAACGAATGGTTTCGGCAGCTGTGGCGGAAAg                  >  2:558364/1‑140 (MQ=255)                                                                                                                    ttGCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCAAAACGAATGGTTTCGGCAGCTGTGGCGGAAAGa                 >  2:945087/1‑140 (MQ=255)                                                                                                                      gCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACTGAGGATAGGAGGCTTCGGTAGCAAAACGAATGGTTTCGGCAGCTGTGGCGGAAAGACt               <  1:999150/140‑1 (MQ=255)                                                                                                                      gCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCAAAACGAATGGTTTCGGCAGCTGTGGCGGaa                    <  1:595268/135‑1 (MQ=255)                                                                                                                      gCATCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCAAAACGAATGGTTTCGGCAGCTGTGGCGGaa                    >  2:595268/1‑135 (MQ=255)                                                                                                                        aTCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCAAAACGAATGGTTTCGGCAGCTGTGGCGGAAAGACTaa             >  2:200448/1‑140 (MQ=255)                                                                                                                        aTCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCAAAACGAATGGTTTCGGCAGCTGTGGCGGAAAGACTaa             <  2:246855/140‑1 (MQ=255)                                                                                                                        aTCAATCTCTTTACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCAAAACGAATGGTTTCGGCAGCTGTGGCGGAAAGACTaa             >  1:637615/1‑140 (MQ=255)                                                                                                                                 tttACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCAAAACGAATGGTTTCGGCAGCTGTGGCGGAAAGACTAAAACCTGCaa    >  2:8630/1‑140 (MQ=255)                                                                                                                                   tACACGGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCAAAACGAATGGTTTCGGCAGCTGTGGCGGAAAGACTAAAACCTGCAAtt  >  2:248101/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       AGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACAGCGCTTGTGCCAGGTCGACGTCAAAACCAACGATCTGGTTGTTTGCATCAATCGATTCAAACGGAGGATAGGAGGCTTCGGTAGCAAAACGAATGGTTTCGGCAGCTGTGGCGGAAAGACTAAAACCTGCAATT  >  NC_000913/902693‑902962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑