Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	909,556	A→G	G498G (GGT→GGC)	poxB ←	pyruvate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	909,556	0	A	G	100.0%	188.6 / NA	53	G498G (GGT→GGC)	poxB	pyruvate oxidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/24);  total (29/24)

TTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCA  >  NC_000913/909419‑909692                                                                                                                                          |                                                                                                                                         ttGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGcc                                                                                                                                        >  2:373751/1‑140 (MQ=255) ttGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGcc                                                                                                                                        >  2:295046/1‑140 (MQ=255)        gTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATg                                                                                                                                 >  1:402761/1‑140 (MQ=255)         ttCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGc                                                                                                                                >  1:843058/1‑140 (MQ=255)           cGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGTGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCg                                                                                                                              >  2:684234/1‑140 (MQ=255)           cGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCg                                                                                                                              <  2:360955/140‑1 (MQ=255)           cGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCg                                                                                                                              <  2:402761/140‑1 (MQ=255)             aGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGTACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCa                                                                                                                            <  1:689565/140‑1 (MQ=255)               tttGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAGCACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACg                                                                                                                          <  1:145267/140‑1 (MQ=255)                   aTCTGCGGTGGAATGGCTAACTCTCCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTc                                                                                                                      >  1:824836/1‑140 (MQ=255)                        cGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCaa                                                                                                                 >  1:724383/1‑140 (MQ=255)                               aTGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGc                                                                                                          <  1:653702/140‑1 (MQ=255)                                     aaCTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGtt                                                                                                    >  1:900739/1‑140 (MQ=255)                                        tcttctTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTtgt                                                                                                 <  1:337352/140‑1 (MQ=255)                                         cttcttTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTtgtg                                                                                                <  2:724383/140‑1 (MQ=255)                                         cttcttTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTtgtg                                                                                                <  1:902659/140‑1 (MQ=255)                                           tcttTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTc                                                                                              >  1:819156/1‑140 (MQ=255)                                              ttGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGTGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCgtg                                                                                           <  1:684234/140‑1 (MQ=255)                                                  cGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGt                                                                                       >  2:137079/1‑140 (MQ=255)                                                       accacATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGt                                                                                  >  2:987387/1‑140 (MQ=255)                                                            aTCCACCAACACCGGACCGTCGATAGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCgt                                                                             <  1:911676/140‑1 (MQ=255)                                                               caccaACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCgtcag                                                                          >  2:649087/1‑140 (MQ=255)                                                                   aaCACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCaa                                                                      >  2:405680/1‑140 (MQ=255)                                                                      acCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATa                                                                   >  1:484983/1‑140 (MQ=255)                                                                      acCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATa                                                                   <  1:446692/140‑1 (MQ=255)                                                                      acCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATa                                                                   <  2:824836/140‑1 (MQ=255)                                                                        cGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGc                                                                 >  2:546780/1‑140 (MQ=255)                                                                          gACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGcca                                                               >  1:119461/1‑140 (MQ=255)                                                                           aCCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGccac                                                              >  1:567378/1‑140 (MQ=255)                                                                              gTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAg                                                           <  1:649087/140‑1 (MQ=255)                                                                               tCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAg                                                           <  2:870252/139‑1 (MQ=255)                                                                               tCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAg                                                           >  1:870252/1‑139 (MQ=255)                                                                               tCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGc                                                          >  2:567803/1‑140 (MQ=255)                                                                                   tGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTc                                                      <  2:119461/140‑1 (MQ=255)                                                                                    ggAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCa                                                     >  1:665660/1‑140 (MQ=255)                                                                                         aGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTcc                                                >  2:544676/1‑140 (MQ=255)                                                                                           gcgcGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCAt                                              <  1:655820/140‑1 (MQ=255)                                                                                           gcgcGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCAt                                              >  2:655820/1‑140 (MQ=255)                                                                                                  gCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCcaca                                       <  2:567378/140‑1 (MQ=255)                                                                                                  gCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCcaca                                       <  1:235785/140‑1 (MQ=255)                                                                                                    aGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACaaa                                     >  2:423831/1‑140 (MQ=255)                                                                                                           catcaACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGc                              >  2:528674/1‑140 (MQ=255)                                                                                                           catcaACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGc                              >  1:573329/1‑140 (MQ=255)                                                                                                            atcaACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCa                             <  1:546780/140‑1 (MQ=255)                                                                                                              caACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACg                           <  2:484983/140‑1 (MQ=255)                                                                                                              caACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACg                           <  1:528674/140‑1 (MQ=255)                                                                                                               aaCTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGTACGc                          <  2:665660/140‑1 (MQ=255)                                                                                                               aaCTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGc                          <  2:573329/140‑1 (MQ=255)                                                                                                                  ttCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCtgt                       >  1:426814/1‑140 (MQ=255)                                                                                                                                   cacGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAAtt      >  1:235013/1‑140 (MQ=255)                                                                                                                                     cGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAAtttt    >  1:602593/1‑140 (MQ=255)                                                                                                                                     cGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAAtttt    >  1:228737/1‑140 (MQ=255)                                                                                                                                       gATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCa  <  1:137079/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         TTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCA  >  NC_000913/909419‑909692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑