Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	992,903	T→C	V761A (GTG→GCG)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	992,903	0	T	C	100.0%	129.9 / NA	37	V761A (GTG→GCG)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/21);  total (16/21)

TTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGCCGAAGATGGCAGCGGTTAC  >  NC_000913/992771‑993036                                                                                                                                     |                                                                                                                                      tttCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGgag                                                                                                                                <  1:507317/140‑1 (MQ=255)    cTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCCGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACg                                                                                                                             <  1:102747/140‑1 (MQ=255)         gcggTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTgcg                                                                                                                        <  1:229697/140‑1 (MQ=255)           ggTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGcgcg                                                                                                                      <  1:676751/140‑1 (MQ=255)            gTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCgg                                                                                                                     <  1:496878/140‑1 (MQ=255)             ttGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGc                                                                                                                    <  2:299589/140‑1 (MQ=255)             ttGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGc                                                                                                                    <  1:473244/140‑1 (MQ=255)              tGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGcc                                                                                                                   <  1:983403/140‑1 (MQ=255)                    acaGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTgc                                                                                                             <  1:579581/140‑1 (MQ=255)                        cTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGcc                                                                                                                   <  1:124309/130‑1 (MQ=255)                        cTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGcc                                                                                                                   >  2:747907/1‑130 (MQ=255)                        cTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGcc                                                                                                                   >  2:124309/1‑130 (MQ=255)                        cTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGcc                                                                                                                   <  1:747907/130‑1 (MQ=255)                             ttGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCt                                                                                                    <  2:990945/140‑1 (MQ=255)                                  gTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCAtt                                                                                                <  1:479349/139‑1 (MQ=255)                                  gTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCAtt                                                                                                >  2:479349/1‑139 (MQ=255)                                     aCGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTAcc                                                                                            <  1:225579/140‑1 (MQ=255)                                          cTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAg                                                                                       <  1:493936/140‑1 (MQ=255)                                                       acgacgACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGt                                                                          >  1:792681/1‑140 (MQ=255)                                                                aGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTAttcgttcg                                                                 <  1:877767/140‑1 (MQ=255)                                                                   ggCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTg                                                              >  1:137495/1‑140 (MQ=255)                                                                                  tGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCg                                               >  1:190105/1‑140 (MQ=255)                                                                                     tGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGgc                                            >  2:943259/1‑140 (MQ=255)                                                                                     tGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGgc                                            >  1:501648/1‑140 (MQ=255)                                                                                         ggATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGgcagca                                        >  1:478480/1‑140 (MQ=255)                                                                                                    ttATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCg                             >  2:143821/1‑140 (MQ=255)                                                                                                    ttATCCTGCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCg                             >  1:231292/1‑140 (MQ=255)                                                                                                           gCAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATg                      >  1:98892/1‑140 (MQ=255)                                                                                                            cAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGc                     >  1:983166/1‑140 (MQ=255)                                                                                                            cAAGCCACCAGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGc                     >  1:840506/1‑140 (MQ=255)                                                                                                                 caccaGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGCCGAAg                >  1:479328/1‑140 (MQ=255)                                                                                                                     aGCCCGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGCCGAAGATgg            <  2:501648/140‑1 (MQ=255)                                                                                                                        ccGGCGGCGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGCCGAAGATGGCAg         <  2:479328/140‑1 (MQ=255)                                                                                                                          ggcggcGAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGCCGAAGATGGCAGCg       <  2:190105/140‑1 (MQ=255)                                                                                                                           gcggcgAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGCCGAAGATGGCAGCgg      >  1:603891/1‑140 (MQ=255)                                                                                                                            cggcgAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGCCGAAGATGGCAGCGGt     <  2:137495/140‑1 (MQ=255)                                                                                                                               cgAATGCGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGCCGAAGATGGCAGCGGTTAc  <  2:719992/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      TTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAACCGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGCCGAAGATGGCAGCGGTTAC  >  NC_000913/992771‑993036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑