Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,061,940	C→T	P48S (CCG→TCG)	torD →	trimethylamine‑N‑oxide reductase‑specific chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,061,940	0	C	T	100.0%	162.3 / NA	47	P48S (CCG→TCG)	torD	trimethylamine‑N‑oxide reductase‑specific chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/24);  total (23/24)

ACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCT  >  NC_000913/1061802‑1062070                                                                                                                                           |                                                                                                                                   aCCACGCTGACAGCACAACAGATTGCC‑‑TGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAAtcgc                                                                                                                                 >  1:23993/1‑140 (MQ=255)       cTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCt                                                                                                                             <  1:641501/140‑1 (MQ=255)                caacaGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTgcggc                                                                                                                    >  2:883263/1‑140 (MQ=255)                    aGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTg                                                                                                                >  2:311282/1‑140 (MQ=255)                        tGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACg                                                                                                            >  1:775785/1‑140 (MQ=255)                           cTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCATTGCGGCGGTGAACGAGc                                                                                                         >  2:754532/1‑140 (MQ=255)                                tttACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGaa                                                                                                    <  1:838743/140‑1 (MQ=255)                                tttACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGaa                                                                                                    >  2:623098/1‑140 (MQ=255)                                    cGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAAcc                                                                                                >  2:127705/1‑140 (MQ=255)                                    cGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAAcc                                                                                                >  1:715250/1‑140 (MQ=255)                                         ggCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTAtt                                                                                           <  1:623098/140‑1 (MQ=255)                                             aGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCa                                                                                       <  2:241392/140‑1 (MQ=255)                                              gcgcAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCAc                                                                                      <  1:981884/140‑1 (MQ=255)                                              gcgcAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCAc                                                                                      >  1:38542/1‑140 (MQ=255)                                               cgcAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACg                                                                                     >  1:768317/1‑140 (MQ=255)                                                 cAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCt                                                                                   <  1:812131/140‑1 (MQ=255)                                                         ctcCCGTGAGCTGGACGATGAACAACTGACGCAAATAGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGa                                                                                 <  1:936648/134‑1 (MQ=255)                                                         ctcCCGTGAGCTGGACGATGAACAACTGACGCAAATAGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGa                                                                                 >  2:936648/1‑134 (MQ=255)                                                            ccGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTg                                                                        >  1:815508/1‑140 (MQ=255)                                                            ccGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTg                                                                        >  1:469578/1‑140 (MQ=255)                                                               tGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACg                                                                     >  1:605500/1‑140 (MQ=255)                                                                   cTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGc                                                                 <  2:469578/140‑1 (MQ=255)                                                                   cTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGc                                                                 <  1:622665/140‑1 (MQ=255)                                                                   cTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGc                                                                 <  1:883263/140‑1 (MQ=255)                                                                       aCGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCATTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGt                                                             <  1:754532/140‑1 (MQ=255)                                                                           tGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTgg                                                         >  2:63616/1‑140 (MQ=255)                                                                             aacaacTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGaa                                                       <  1:911978/140‑1 (MQ=255)                                                                              acaacTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAAc                                                      <  2:715250/140‑1 (MQ=255)                                                                                  cTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGc                                                  >  1:119416/1‑140 (MQ=255)                                                                                  cTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGc                                                  >  1:127610/1‑140 (MQ=255)                                                                                     aCGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCcgc                                               >  2:606881/1‑140 (MQ=255)                                                                                      cGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGGCGATGCCCGTCTGGAACTGGCcgcg                                              <  1:752876/140‑1 (MQ=255)                                                                                      cGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCcgcg                                              <  2:127610/140‑1 (MQ=255)                                                                                                 aGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCgg                                   <  2:249486/140‑1 (MQ=255)                                                                                                 aGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCgg                                   <  2:775785/140‑1 (MQ=255)                                                                                                  gTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGc                                  <  1:937987/140‑1 (MQ=255)                                                                                                       cAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGtt                             >  2:645983/1‑140 (MQ=255)                                                                                                            ggCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCtga                        >  1:932583/1‑140 (MQ=255)                                                                                                             gCTGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCtgat                       >  2:525803/1‑140 (MQ=255)                                                                                                               tGAATGGTTTTCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCtgatga                     >  1:64771/1‑140 (MQ=255)                                                                                                                      ttttCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCtgat                       <  2:899420/131‑1 (MQ=255)                                                                                                                      ttttCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCtgat                       >  1:899420/1‑131 (MQ=255)                                                                                                                      ttttCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGacaa              <  2:932583/140‑1 (MQ=255)                                                                                                                        ttCGTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGacaaac            >  1:697981/1‑140 (MQ=255)                                                                                                                           gTTGCTGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAg         <  1:799703/140‑1 (MQ=255)                                                                                                                                tGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAgcggcg    <  2:64771/140‑1 (MQ=255)                                                                                                                                tGAAAAGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAgcggcg    <  1:525803/140‑1 (MQ=255)                                                                                                                                  aaaaGCGAATCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCt  <  1:63616/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                   ACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCT  >  NC_000913/1061802‑1062070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑