Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,143,140	T→C	T410A (ACT→GCT)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,143,140	0	T	C	100.0%	125.5 / NA	36	T410A (ACT→GCT)	rne	ribonuclease E
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/17);  total (19/17)

TTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTT  >  NC_000913/1143004‑1143274                                                                                                                                         |                                                                                                                                       ttttCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCAcc                                                                                                                                     >  2:812228/1‑140 (MQ=255)   ttcattcaGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAg                                                                                                                                   >  2:857655/1‑140 (MQ=255)         cagcagGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGaacaac                                                                                                                             <  2:912793/140‑1 (MQ=255)                   gaagCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAAc                                                                                                                   <  1:753985/140‑1 (MQ=255)                           tGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGAc                                                                                                           <  2:587440/140‑1 (MQ=255)                                caGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGAt                                                                                                      <  2:188030/140‑1 (MQ=255)                                     aCAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCAcc                                                                                                 <  2:973313/140‑1 (MQ=255)                                     aCAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCAcc                                                                                                 <  1:857655/140‑1 (MQ=255)                                             gTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATg                                                                                         >  1:891667/1‑140 (MQ=255)                                                        gggCGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGgcgc                                                                              >  2:451633/1‑140 (MQ=255)                                                           tgtTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGa                                                                           >  1:449139/1‑140 (MQ=255)                                                             ttCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGAGCTCAGGCGCTGACg                                                                         >  2:413856/1‑140 (MQ=255)                                                               ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATg                                                                                         >  2:134840/1‑122 (MQ=255)                                                               ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATg                                                                                         <  1:134840/122‑1 (MQ=255)                                                               ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACggg                                                                       <  1:451633/140‑1 (MQ=255)                                                               ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACggg                                                                       <  2:891667/140‑1 (MQ=255)                                                                                    gATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGc                                                  <  2:449139/140‑1 (MQ=255)                                                                                     aTCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCg                                                 >  2:278063/1‑140 (MQ=255)                                                                                        aGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCgaga                                              >  2:511970/1‑140 (MQ=255)                                                                                        aGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCgaga                                              >  1:367649/1‑140 (MQ=255)                                                                                         gACGCAGAATAGAGAGCGACAGCGATTCGTTGACACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGaa                                             >  2:975347/1‑140 (MQ=255)                                                                                               gAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATgg                                       <  1:511970/140‑1 (MQ=255)                                                                                                aaTAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGc                                      <  1:993178/140‑1 (MQ=255)                                                                                                    gagagCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGAt                                  >  2:200937/1‑140 (MQ=255)                                                                                                       agCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTg                               >  1:657860/1‑140 (MQ=255)                                                                                                        gCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGa                              >  2:135460/1‑140 (MQ=255)                                                                                                          gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAAt                            >  1:442165/1‑140 (MQ=255)                                                                                                          gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAG‑ACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTAACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATa                           >  1:990432/1‑140 (MQ=255)                                                                                                               cGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATAcgcg                       <  2:912925/140‑1 (MQ=255)                                                                                                                 aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                     <  1:440881/140‑1 (MQ=255)                                                                                                                 aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                     >  1:391613/1‑140 (MQ=255)                                                                                                                 aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                     <  1:135460/140‑1 (MQ=255)                                                                                                                 aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGAGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                     <  1:413856/140‑1 (MQ=255)                                                                                                                            cgcacgGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACg          >  2:506836/1‑140 (MQ=255)                                                                                                                                   tGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCt   >  2:712011/1‑140 (MQ=255)                                                                                                                                    gCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCtt  <  1:506836/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       TTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTT  >  NC_000913/1143004‑1143274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑