Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	231,834	A→G	N238S (AAC→AGC)	yafD →	endonuclease/exonuclease/phosphatase domain‑containing protein YafD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	231,834	0	A	G	100.0%	155.6 / NA	45	N238S (AAC→AGC)	yafD	endonuclease/exonuclease/phosphatase domain‑containing protein YafD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/27);  total (18/27)

CCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGG  >  NC_000913/231699‑231960                                                                                                                                        |                                                                                                                               ccTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGTGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTc                                                                                                                            >  1:451106/1‑140 (MQ=255)   tGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAg                                                                                                                          >  2:551823/1‑140 (MQ=255)      aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGTGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGa                                                                                                                       <  2:451106/140‑1 (MQ=255)      aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCTCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGa                                                                                                                       <  1:710951/140‑1 (MQ=255)      aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGa                                                                                                                       <  2:133966/140‑1 (MQ=255)      aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGa                                                                                                                       <  2:156437/140‑1 (MQ=255)      aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGa                                                                                                                       <  2:42520/140‑1 (MQ=255)           tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCtt                                                                                                                  >  2:888035/1‑140 (MQ=255)                ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTa                                                                                                             <  2:372735/140‑1 (MQ=255)                               atCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCt                                                                                              <  2:280578/140‑1 (MQ=255)                                tCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCtt                                                                                             <  1:888035/140‑1 (MQ=255)                                   cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCg                                                                                          >  1:435093/1‑140 (MQ=255)                                     ttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGAt                                                                                        <  2:480741/140‑1 (MQ=255)                                      tGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTc                                                                                            >  2:107504/1‑135 (MQ=255)                                      tGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTc                                                                                            <  1:107504/135‑1 (MQ=255)                                      tGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATc                                                                                       >  1:824871/1‑140 (MQ=255)                                      tGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATc                                                                                       >  1:218856/1‑140 (MQ=255)                                      tGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATc                                                                                       >  2:403652/1‑140 (MQ=255)                                        cgcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGAt                                                                                        <  2:723633/137‑1 (MQ=255)                                        cgcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGAt                                                                                        >  1:723633/1‑137 (MQ=255)                                        cgcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATcac                                                                                     >  2:862268/1‑140 (MQ=255)                                          cgGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACaa                                                                                   <  2:218856/140‑1 (MQ=255)                                              aaaTGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCg                                                                               <  1:846810/140‑1 (MQ=255)                                               aaTGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGc                                                                              <  2:824871/140‑1 (MQ=255)                                                  gTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTAc                                                                           <  1:210411/140‑1 (MQ=255)                                                       tGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGtt                                                                      >  1:909137/1‑140 (MQ=255)                                                               gTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAg                                                              <  1:558780/140‑1 (MQ=255)                                                                          cGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGc                                                   <  2:79458/140‑1 (MQ=255)                                                                          cGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGc                                                   <  1:535302/140‑1 (MQ=255)                                                                             tgatCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTg                                                <  1:855771/140‑1 (MQ=255)                                                                               atCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGat                                              >  2:729154/1‑140 (MQ=255)                                                                                tCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGata                                             <  1:403652/140‑1 (MQ=255)                                                                                   gcgcCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaat                                          >  1:286053/1‑140 (MQ=255)                                                                                     gcCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa                                        <  2:909137/140‑1 (MQ=255)                                                                                       cGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAgg                                      <  1:862268/140‑1 (MQ=255)                                                                                         tCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTa                                    >  2:860379/1‑140 (MQ=255)                                                                                           gggCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATg                                  >  1:285216/1‑140 (MQ=255)                                                                                              cGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCa                               >  2:489442/1‑140 (MQ=255)                                                                                               gTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAg                              <  1:415231/140‑1 (MQ=255)                                                                                                  tGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc                           <  1:489442/140‑1 (MQ=255)                                                                                                  tGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc                           <  1:551823/140‑1 (MQ=255)                                                                                                         ccGCTCGATTTTGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCcaca                    <  2:435093/140‑1 (MQ=255)                                                                                                                 ttttGTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGAc            <  1:630893/140‑1 (MQ=255)                                                                                                                     gTTTTCTACCGTGGTCTGAGCGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAAc        >  1:508859/1‑140 (MQ=255)                                                                                                                           tACCGTGTTCTGAGCGTCAGGGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAACTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTgg  >  1:755202/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                               CCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGG  >  NC_000913/231699‑231960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑