Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,195,769	A→G	Y216C (TAC→TGC)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,195,769	0	A	G	100.0%	179.1 / NA	51	Y216C (TAC→TGC)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/22);  total (29/22)

CGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATACGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGAAGAGTTTGGCGGTGAACTGATCGACGG  >  NC_000913/1195632‑1195901                                                                                                                                          |                                                                                                                                     cGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCg                                                                                                                                    <  1:649394/140‑1 (MQ=255)     gCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAAt                                                                                                                                >  2:778262/1‑140 (MQ=255)          gaAAGTGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTa                                                                                                                           <  1:20310/140‑1 (MQ=255)          gaAAGTGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTa                                                                                                                           >  2:531893/1‑140 (MQ=255)           aaaGTGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTaa                                                                                                                          <  1:783310/140‑1 (MQ=255)            aaGTGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAAc                                                                                                                         <  1:256092/140‑1 (MQ=255)               tGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGAt                                                                                                                      >  2:921000/1‑140 (MQ=255)               tGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGAt                                                                                                                      >  2:293684/1‑140 (MQ=255)                   tAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCgtg                                                                                                                  >  1:275157/1‑140 (MQ=255)                         ccTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCgtgactctg                                                                                                            >  1:770183/1‑140 (MQ=255)                              gTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCgtgactctgtgact                                                                                                       <  1:293684/140‑1 (MQ=255)                              gTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCgtgactctgtgact                                                                                                       <  1:900295/140‑1 (MQ=255)                                      aTGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGca                                                                                               >  1:362221/1‑140 (MQ=255)                                       tGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGcac                                                                                              >  1:852766/1‑140 (MQ=255)                                        gggggTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGcaca                                                                                             >  2:299914/1‑140 (MQ=255)                                           ggTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAg                                                                                          >  1:560994/1‑140 (MQ=255)                                            gTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAgg                                                                                         >  1:916416/1‑140 (MQ=255)                                            gTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAgg                                                                                         <  2:560994/140‑1 (MQ=255)                                             tGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGTCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTC‑‑TGACTCTGGTGCACAAAGGCaa                                                                                      >  1:786275/1‑140 (MQ=255)                                              gaagaaAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCa                                                                                       <  2:770183/140‑1 (MQ=255)                                                agaaAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAAc                                                                                     >  2:956594/1‑140 (MQ=255)                                                                    cATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAgg                                                                 <  1:778262/140‑1 (MQ=255)                                                                    cATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAgg                                                                 <  2:362221/140‑1 (MQ=255)                                                                     aTTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGa                                                                <  1:299914/140‑1 (MQ=255)                                                                      ttGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAg                                                               <  1:956594/140‑1 (MQ=255)                                                                        gtgGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCg                                                             >  1:776438/1‑140 (MQ=255)                                                                            tATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTa                                                         >  1:691201/1‑140 (MQ=255)                                                                            tATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTa                                                         >  2:823709/1‑140 (MQ=255)                                                                              tCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTaaa                                                       >  2:644946/1‑140 (MQ=255)                                                                                 gTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGAc                                                    >  2:178734/1‑140 (MQ=255)                                                                                 gTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGAc                                                    >  1:327776/1‑140 (MQ=255)                                                                                  tATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACt                                                   <  1:921000/140‑1 (MQ=255)                                                                                      aaGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTgggg                                               >  1:424828/1‑140 (MQ=255)                                                                                         ccGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTa                                            >  1:481774/1‑140 (MQ=255)                                                                                            tgtTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACTATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCt                                             <  2:870929/136‑1 (MQ=255)                                                                                            tgtTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACTATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCt                                             >  1:870929/1‑136 (MQ=255)                                                                                            tgtTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCa                                         >  2:453132/1‑140 (MQ=255)                                                                                            tgtTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCa                                         >  2:191132/1‑140 (MQ=255)                                                                                            tgtTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCa                                         >  2:776430/1‑140 (MQ=255)                                                                                            tgtTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCa                                         >  1:568400/1‑140 (MQ=255)                                                                                             gtTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAg                                        <  2:776438/140‑1 (MQ=255)                                                                                                 ggAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTgg                                    >  1:191086/1‑140 (MQ=255)                                                                                                   aagaagGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGgcg                                  <  1:482305/140‑1 (MQ=255)                                                                                                        ggCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGa                             <  1:178734/140‑1 (MQ=255)                                                                                                                        ttCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGAAGAGTTTGGCGGTGaa             <  1:342292/140‑1 (MQ=255)                                                                                                                          cGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGAAGAGTTTGGCg                  >  2:11244/1‑133 (MQ=255)                                                                                                                          cGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGAAGAGTTTGGCg                  <  1:11244/133‑1 (MQ=255)                                                                                                                             gCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGAAGAGTTTGGCGGTGAACTGAt        <  2:691201/140‑1 (MQ=255)                                                                                                                             gCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGAAGAGTTTGGCGGTGAACTGAt        <  1:776430/140‑1 (MQ=255)                                                                                                                               aGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGAAGAGTTTGGCGGTGAACTGATCg      >  2:718474/1‑140 (MQ=255)                                                                                                                                   aTCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGAAGAGTTTGGCGGTGAACTGATCGACgg  <  2:327776/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     CGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATACGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGAAGAGTTTGGCGGTGAACTGATCGACGG  >  NC_000913/1195632‑1195901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑