Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,336,072	A→G	Y81C (TAC→TGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,336,072	0	A	G	100.0%	164.5 / NA	48	Y81C (TAC→TGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/24);  total (24/24)

CGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTACCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAACCCGCTCTCACCGGTCGACCTGG  >  NC_000913/1335939‑1336206                                                                                                                                      |                                                                                                                                       cGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGccc                                                                                                                                  <  1:247289/140‑1 (MQ=255)    cTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGc                                                                                                                               >  1:137200/1‑140 (MQ=255)    cTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGc                                                                                                                               >  2:815079/1‑140 (MQ=255)    cTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGc                                                                                                                               >  1:344533/1‑140 (MQ=255)      aCCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCaa                                                                                                                             <  1:102276/140‑1 (MQ=255)      aCCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCaa                                                                                                                             >  1:535342/1‑140 (MQ=255)       cccAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAg                                                                                                                            <  2:749662/140‑1 (MQ=255)           aGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTg                                                                                                                        >  2:431014/1‑140 (MQ=255)             tCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTG‑AAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTg                                                                                                                     >  1:482934/1‑140 (MQ=255)                          tGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACttt                                                                                                         >  1:867060/1‑140 (MQ=255)                               gAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCgg                                                                                                    >  2:141181/1‑140 (MQ=255)                                     ctgctgCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTAcc                                                                                              <  1:431014/140‑1 (MQ=255)                                     ctgctgCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTAcc                                                                                              <  2:975198/140‑1 (MQ=255)                                       gctgcGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTg                                                                                            <  1:638334/140‑1 (MQ=255)                                        ctgcGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGc                                                                                           <  1:56588/140‑1 (MQ=255)                                        ctgcGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGc                                                                                           <  1:56587/140‑1 (MQ=255)                                          gcgcTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGACCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCg                                                                                         <  2:179170/140‑1 (MQ=255)                                             cTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCgttg                                                                                      >  1:675792/1‑140 (MQ=255)                                                                  tACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGcgcg                                                                 <  2:137200/140‑1 (MQ=255)                                                                  tACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGcgcg                                                                 >  2:176276/1‑140 (MQ=255)                                                                  tACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGcgcg                                                                 >  1:416470/1‑140 (MQ=255)                                                                  tACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGcgcg                                                                 <  2:683263/140‑1 (MQ=255)                                                                   aCCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGa                                                                <  2:784041/140‑1 (MQ=255)                                                                   aCCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGa                                                                >  2:41409/1‑140 (MQ=255)                                                                    ccGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGaa                                                               >  2:740451/1‑140 (MQ=255)                                                                      gAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGc                                                             <  2:39263/140‑1 (MQ=255)                                                                       aaGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCg                                                            >  2:957208/1‑140 (MQ=255)                                                                                   aCGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTc                                                <  2:675792/140‑1 (MQ=255)                                                                                    cgcgCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGcc                                                  <  1:430639/137‑1 (MQ=255)                                                                                    cgcgCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGcc                                                  >  2:430639/1‑137 (MQ=255)                                                                                    cgcgCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTcg                                               >  2:669749/1‑140 (MQ=255)                                                                                       gcTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTcggcg                                            >  2:405046/1‑140 (MQ=255)                                                                                         tGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCggcggc                                          >  1:524125/1‑140 (MQ=255)                                                                                           gCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAAc                                                     <  2:28726/127‑1 (MQ=255)                                                                                           gCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAAc                                                     >  1:28726/1‑127 (MQ=255)                                                                                           gCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGa                                        <  1:41409/140‑1 (MQ=255)                                                                                           gCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGa                                        <  1:176276/140‑1 (MQ=255)                                                                                                 tGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGc                                  <  2:524125/140‑1 (MQ=255)                                                                                                 tGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGc                                  <  2:344533/140‑1 (MQ=255)                                                                                                 tGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGc                                  >  1:719487/1‑140 (MQ=255)                                                                                                          aaTGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTaa                         >  2:695091/1‑140 (MQ=255)                                                                                                             gCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAAccc                      >  1:612476/1‑140 (MQ=255)                                                                                                                 aTGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAACCCGctc                  >  2:112642/1‑140 (MQ=255)                                                                                                                   gCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAACCCGCtctc                <  2:535342/140‑1 (MQ=255)                                                                                                                       aCCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAACCCGCTCTCACCg            >  2:413659/1‑140 (MQ=255)                                                                                                                        ccGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAACCCGCTCTCACCgg           <  2:769177/140‑1 (MQ=255)                                                                                                                         cGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAACCCGCTCTCACCGGt          <  1:957208/140‑1 (MQ=255)                                                                                                                          gTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAACCCGCTCTCACCGGTc         <  2:719487/140‑1 (MQ=255)                                                                                                                                 tgcctgccGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAACCCGCTCTCACCGGTCGACCTgg  <  1:695091/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       CGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTACCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAACCCGCTCTCACCGGTCGACCTGG  >  NC_000913/1335939‑1336206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑