Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,385,070	T→C	R318R (CGT→CGC)	ycjX →	DUF463 domain‑containing protein YcjX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,385,070	0	T	C	100.0%	166.0 / NA	48	R318R (CGT→CGC)	ycjX	DUF463 domain‑containing protein YcjX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/20);  total (28/20)

GAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGTACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGCAACAACTGATTCAGGATGCCTG  >  NC_000913/1384935‑1385201                                                                                                                                        |                                                                                                                                    gAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCAccc                                                                                                                                 >  1:150429/1‑140 (MQ=255) gAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCAccc                                                                                                                                 >  1:499313/1‑140 (MQ=255)            tGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCgt                                                                                                                      >  1:195602/1‑140 (MQ=255)            tGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCAGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCgt                                                                                                                      >  2:874872/1‑140 (MQ=255)                cTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCgtttgt                                                                                                                  >  1:320923/1‑140 (MQ=255)                   tGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGtttt                                                                                                               >  1:841140/1‑140 (MQ=255)                      ccGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGc                                                                                                            <  2:681514/140‑1 (MQ=255)                               tgtgCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCg                                                                                                   <  2:816699/140‑1 (MQ=255)                                gtgCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGa                                                                                                  <  2:934325/140‑1 (MQ=255)                                gtgCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGa                                                                                                  <  2:841140/140‑1 (MQ=255)                                 tgCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGAt                                                                                                 >  1:535600/1‑140 (MQ=255)                                 tgCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGAt                                                                                                 <  2:195602/140‑1 (MQ=255)                                    tggtggATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAg                                                                                              <  2:320923/140‑1 (MQ=255)                                      gtggATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCt                                                                                            >  2:786264/1‑140 (MQ=255)                                      gtggATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCt                                                                                            >  2:142670/1‑140 (MQ=255)                                          aTTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTg                                                                                        >  1:469473/1‑140 (MQ=255)                                          aTTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTg                                                                                        <  1:874872/140‑1 (MQ=255)                                               cTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTtgc                                                                                   <  1:89155/140‑1 (MQ=255)                                                        ctcAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGTGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTaa                                                                          >  1:878055/1‑140 (MQ=255)                                                        ctcAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTaa                                                                          >  2:265108/1‑140 (MQ=255)                                                                 gggCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCa                                                                 >  2:121980/1‑140 (MQ=255)                                                                  ggCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCAt                                                                >  1:167221/1‑140 (MQ=255)                                                                  ggCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCAt                                                                >  1:303287/1‑140 (MQ=255)                                                                  ggCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCAt                                                                <  2:167221/140‑1 (MQ=255)                                                                  ggCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCAt                                                                >  2:661060/1‑140 (MQ=255)                                                                                    atatGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCa                                                         <  1:347671/129‑1 (MQ=255)                                                                                    atatGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCa                                                         >  2:347671/1‑129 (MQ=255)                                                                                    atatGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCa                                               <  1:563038/139‑1 (MQ=255)                                                                                    atatGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCa                                               >  2:563038/1‑139 (MQ=255)                                                                                    atatGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCAc                                              >  1:332506/1‑140 (MQ=255)                                                                                       tGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAg                                                 <  1:465287/134‑1 (MQ=255)                                                                                       tGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAg                                                 >  2:465287/1‑134 (MQ=255)                                                                                        gCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACg                                             <  1:146658/137‑1 (MQ=255)                                                                                        gCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACg                                             >  2:146658/1‑137 (MQ=255)                                                                                          gTCTGGCACTGACGGAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAat                                        <  1:82295/140‑1 (MQ=255)                                                                                          gTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAat                                        <  2:499313/140‑1 (MQ=255)                                                                                          gTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAat                                        <  1:786264/140‑1 (MQ=255)                                                                                           tCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAata                                       >  2:804535/1‑140 (MQ=255)                                                                                                    gACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCAt                              <  2:303287/140‑1 (MQ=255)                                                                                                      cGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCAtt                             <  2:327024/139‑1 (MQ=255)                                                                                                      cGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCAtt                             >  1:327024/1‑139 (MQ=255)                                                                                                         aGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACTATCGATCAGCACGCTAATATGGTTTCATTACTg                         >  1:686684/1‑140 (MQ=255)                                                                                                         aGCTGATGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTg                         >  1:951608/1‑140 (MQ=255)                                                                                                               tGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGcaacaa                   >  2:651904/1‑140 (MQ=255)                                                                                                               tGCAAAGTTTTCACTACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGcaacaa                   <  1:265108/140‑1 (MQ=255)                                                                                                                             tACGGGCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGCAACAACTGATTCAGGATGc     <  2:150429/140‑1 (MQ=255)                                                                                                                                gggCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGCAACAACTGATTCAGGATGCCTg  >  2:427030/1‑140 (MQ=255)                                                                                                                                gggCAGCGCACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGCAACAACTGATTCAGGATGCCTg  >  2:572759/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    GAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGTACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGCAACAACTGATTCAGGATGCCTG  >  NC_000913/1384935‑1385201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑