Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,418,524	A→G	intergenic (‑295/‑147)	kilR ← / → sieB	Rac prophage; inhibitor of FtsZ, killing protein/Rac prophage; phage superinfection exclusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,418,524	0	A	G	100.0%	211.4 / NA	61	intergenic (‑295/‑147)	kilR/sieB	Rac prophage; inhibitor of FtsZ, killing protein/Rac prophage; phage superinfection exclusion protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/36);  total (25/36)

CATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTGAG  >  NC_000913/1418387‑1418660                                                                                                                                          |                                                                                                                                         cATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGcc                                                                                                                                        >  2:460219/1‑140 (MQ=255) cATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATACCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGcc                                                                                                                                        <  2:719394/140‑1 (MQ=255)   tCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGa                                                                                                                                      <  2:206984/140‑1 (MQ=255)   tCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGa                                                                                                                                      <  2:756354/140‑1 (MQ=255)   tCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGAGCACTCCCACCATTTAAATTTAACAGACAAGGCCGa                                                                                                                                      <  1:337054/140‑1 (MQ=255)    cacaAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGAc                                                                                                                                     <  1:123896/140‑1 (MQ=255)    cacaAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGAc                                                                                                                                     <  2:469210/140‑1 (MQ=255)    cacaAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGAc                                                                                                                                     <  2:479347/140‑1 (MQ=255)    cacaAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGAc                                                                                                                                     <  2:544935/140‑1 (MQ=255)                   aaaaaGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCg                                                                                                                      >  1:514426/1‑140 (MQ=255)                         ggCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATg                                                                                                                <  2:81757/140‑1 (MQ=255)                             gACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGcc                                                                                                            >  1:73888/1‑140 (MQ=255)                              aCATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCt                                                                                                           <  2:525241/140‑1 (MQ=255)                              aCATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCt                                                                                                           >  1:729195/1‑140 (MQ=255)                                        aaCTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACGGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTtgtg                                                                                                 >  1:897267/1‑140 (MQ=255)                                        aaCTCCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTtgtg                                                                                                 >  1:807863/1‑140 (MQ=255)                                              aaGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGt                                                                                           <  1:784038/140‑1 (MQ=255)                                                 aaaaaaaCTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGtttt                                                                                        <  2:830379/140‑1 (MQ=255)                                                   aaaaaCTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTAt                                                                                      <  2:391000/140‑1 (MQ=255)                                                           aTGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTcaccac                                                                              <  1:981485/140‑1 (MQ=255)                                                              ccgccAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTc                                                                           <  1:200750/140‑1 (MQ=255)                                                                          cacacaGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCggtg                                                               >  1:705441/1‑140 (MQ=255)                                                                               aGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGtctc                                                          >  2:455044/1‑140 (MQ=255)                                                                               aGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGtctc                                                          >  2:466625/1‑140 (MQ=255)                                                                               aGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGtctc                                                          >  1:110400/1‑140 (MQ=255)                                                                                gCAATGTCGTTATTTACAACCGGATGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCgg                                                                 >  1:577399/1‑132 (MQ=255)                                                                                gCAATGTCGTTATTTACAACCGGATGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCgg                                                                 <  2:577399/132‑1 (MQ=255)                                                                                  aaTGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGc                                                       <  2:73888/140‑1 (MQ=255)                                                                                    tGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCta                                                     <  1:877652/140‑1 (MQ=255)                                                                                    tGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCta                                                     <  2:729195/140‑1 (MQ=255)                                                                                    tGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCta                                                     <  2:705441/140‑1 (MQ=255)                                                                                      tCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCtata                                                   >  2:478559/1‑140 (MQ=255)                                                                                         ttATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATAccc                                                >  1:357518/1‑140 (MQ=255)                                                                                         ttATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATAccc                                                >  1:968515/1‑140 (MQ=255)                                                                                         ttATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATAccc                                                >  1:773514/1‑140 (MQ=255)                                                                                                 aaCCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCg                                        <  2:152362/140‑1 (MQ=255)                                                                                                     ggAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGagag                                    <  1:757106/140‑1 (MQ=255)                                                                                                      gAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGagag                                    <  2:426889/139‑1 (MQ=255)                                                                                                      gAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGagag                                    >  1:426889/1‑139 (MQ=255)                                                                                                      gAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGc                                   >  1:922274/1‑140 (MQ=255)                                                                                                      gAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGc                                   >  1:911448/1‑140 (MQ=255)                                                                                                      gAGGCGCACTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGc                                   >  2:533480/1‑140 (MQ=255)                                                                                                             aCTCCCACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGtt                            <  2:897267/140‑1 (MQ=255)                                                                                                                cccACCATTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAAc                         >  2:750847/1‑140 (MQ=255)                                                                                                                     caTTTAAATTTAACAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTc                    <  1:903939/140‑1 (MQ=255)                                                                                                                       tttaaatttaaCAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCaa                  >  2:243606/1‑140 (MQ=255)                                                                                                                       tttaaatttaaCAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCaa                  <  2:514426/140‑1 (MQ=255)                                                                                                                       tttaaatttaaCAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCaa                  <  2:911448/140‑1 (MQ=255)                                                                                                                         taaatttaaCAGACACGGCCAACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCACGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATa                >  2:371964/1‑140 (MQ=255)                                                                                                                         taaatttaaCAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTgtgt                             <  2:723991/127‑1 (MQ=255)                                                                                                                         taaatttaaCAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTgtgt                             >  1:723991/1‑127 (MQ=255)                                                                                                                         taaatttaaCAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAGCATTTCAATa                >  1:685028/1‑140 (MQ=255)                                                                                                                             tttaaCAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCCACAGCGAGAGCTTGTGTTAACATTTCAATACCCt            <  1:455044/140‑1 (MQ=255)                                                                                                                              ttaaCAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCtt           <  1:371964/140‑1 (MQ=255)                                                                                                                              ttaaCAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCtt           <  1:750847/140‑1 (MQ=255)                                                                                                                               taaCAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTa          <  2:773514/140‑1 (MQ=255)                                                                                                                                 aCAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTCTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACa        >  2:848019/1‑140 (MQ=255)                                                                                                                                  cAGACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAg       <  1:243606/140‑1 (MQ=255)                                                                                                                                    gACAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTCTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGtt     <  1:848019/140‑1 (MQ=255)                                                                                                                                      cAAGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTga   <  2:807863/140‑1 (MQ=255)                                                                                                                                       aaGGCCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTgag  <  1:381378/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTGAG  >  NC_000913/1418387‑1418660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑