Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,497,929	A→G	K358K (AAA→AAG)	opgD →	glucan biosynthesis protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,497,929	0	A	G	100.0%	149.7 / NA	44	K358K (AAA→AAG)	opgD	glucan biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/26);  total (18/26)

GATTTGCCGTCCGCTGAATAATCCGCAAAAATTGCAGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAACGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCCAACAACGGGCGAAACGCTGGATAACATTGTCTGCTTCTGGCAGCCAGAAAAAGCTGTAAAAGCAG  >  NC_000913/1497794‑1498062                                                                                                                                        |                                                                                                                                      gATTTGCCGTCCGCTGAATAATCCGCAAAAATTGCAGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGcc                                                                                                                                   <  1:150926/140‑1 (MQ=255) gATTTGCCGTCCGCTGAATAATCCGCAAAAATTGCAGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGcc                                                                                                                                   <  2:620581/140‑1 (MQ=255)  aTTTGCCGTCCGCTGAATAATCCGCAAAAATTGCAGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGccc                                                                                                                                  <  2:974591/140‑1 (MQ=255)        cGTCCGCTGAATAATCCGCAAAAATTGCAGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGGAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCt                                                                                                                            <  1:976128/140‑1 (MQ=255)        cGTCCGCTGAATAATCCGCAAAAATTGCAGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCt                                                                                                                            <  1:493894/140‑1 (MQ=255)        cGTCCGCTGAATAATCCGCAAAAATTGCAGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCt                                                                                                                            <  1:51470/140‑1 (MQ=255)        cGTCCGCTGAATAATCCGCAAAAATTGCAGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCt                                                                                                                            <  1:334446/140‑1 (MQ=255)          tCCGCTGAATAATCCGCAAAAATTGCAGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCtgt                                                                                                                          <  2:639928/140‑1 (MQ=255)          tCCGCTGAATAATCCGCAAAAATTGCAGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCtgt                                                                                                                          <  1:615326/140‑1 (MQ=255)              cTGAATAATCCGCAAAAATTGCAGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGt                                                                                                                              <  1:433500/132‑1 (MQ=255)              cTGAATAATCCGCAAAAATTGCAGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGt                                                                                                                              >  2:433500/1‑132 (MQ=255)                        cGCAAAAATTGCAGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGt                                                                                                            <  1:952702/140‑1 (MQ=255)                           aaaaaTTGCAGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTaa                                                                                                          >  1:58148/1‑139 (MQ=255)                           aaaaaTTGCAGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTaa                                                                                                          <  2:58148/139‑1 (MQ=255)                           aaaaaTTGCAGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAAc                                                                                                         >  1:775463/1‑140 (MQ=255)                                    aGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGt                                                                                                <  1:669043/140‑1 (MQ=255)                                    aGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGt                                                                                                <  2:775463/140‑1 (MQ=255)                                         aaTGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAggg                                                                                           >  2:293950/1‑140 (MQ=255)                                           tGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCa                                                                                         >  2:89384/1‑140 (MQ=255)                                             cTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCAcc                                                                                       >  1:497528/1‑140 (MQ=255)                                                       caacaaCCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGGGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGa                                                                             >  2:675825/1‑140 (MQ=255)                                                         acaacCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATg                                                                           <  1:1000102/140‑1 (MQ=255)                                                          caacCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATgg                                                                          >  2:696530/1‑140 (MQ=255)                                                           aacCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGa                                                                         <  2:517466/140‑1 (MQ=255)                                                           aacCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGa                                                                         <  2:448154/140‑1 (MQ=255)                                                                  aaGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCca                                                                  >  2:828940/1‑140 (MQ=255)                                                                  aaGGGTTTGGTTCATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTTTAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGc                                                                                 >  1:477692/1‑125 (MQ=255)                                                                  aaGGGTTTGGTTCATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTTTAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGc                                                                                 <  2:477692/125‑1 (MQ=255)                                                                            tttATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCCAACAACGGGCg                                                        <  1:828940/140‑1 (MQ=255)                                                                               aTTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCCAACAACGGGCGaaa                                                     <  1:675825/140‑1 (MQ=255)                                                                                       tGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCCAACAACGGGCGAAACGCTGGAt                                             <  2:214529/140‑1 (MQ=255)                                                                                          aTCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCCAACAACGGGCGAAACGCTGGATAAc                                          >  2:474825/1‑140 (MQ=255)                                                                                                   tctcCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCCAACAACGGGCGAAACGCTGGATAACATTGTCTGc                                 >  1:90004/1‑140 (MQ=255)                                                                                                   tctcCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCCAACAACGGGCGAAACGCTGGATAACATTGTCTGc                                 >  2:393987/1‑140 (MQ=255)                                                                                                   tctcCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCCAACAACGGGCGAAACGCTGGATAACATTGTCTGc                                 >  1:961969/1‑140 (MQ=255)                                                                                                     tcCCATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCCAACAACGGGCGAAACGCTGGATAACATTGTCTGCtt                               >  2:36276/1‑140 (MQ=255)                                                                                                      cccATTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCCAACAACGGGCGAAACGCTGGATAACATTGTCTGCTTc                              >  2:378464/1‑140 (MQ=255)                                                                                                         aTTATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCCAACAACGGGCGAAACGCTGGATAACATTGTCTGCTTCTgg                           <  1:393987/140‑1 (MQ=255)                                                                                                          ttATCAGGACATTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCCAACAACGGGCGAAACGCTGGATAACATTGTCTGCTTCTGGc                          <  1:474825/140‑1 (MQ=255)                                                                                                                    aTTATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCCAACAACGGGCGAAACGCTGGATAACATTGTCTGCTTCTGGCAGCCAGaaaa                <  1:378464/140‑1 (MQ=255)                                                                                                                      tATGGGCTGGTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCCAACAACGGGCGAAACGCTGGATAACATTGTCTGCTTCTGGCAGCCAGAAAAAg              >  1:832513/1‑140 (MQ=255)                                                                                                                               gTATAACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCCAACAACGGGCGAAACGCTGGATAACATTGTCTGCTTCTGGCAGCCAGAAAAAGCTGTAAAAg     <  1:696530/140‑1 (MQ=255)                                                                                                                                  taACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCCAACAACGGGCGAAACGCTGGATAACATTGTCTGCTTCTGGCAGCCAGAAAAAGCTGTAAAAGCAg  <  2:33561/140‑1 (MQ=255)                                                                                                                                  taACAAGCGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCCAACAACGGGCGAAACGCTGGATAACATTGTCTGCTTCTGGCAGCCAGAAAAAGCTGTAAAAGCAg  >  1:33561/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      GATTTGCCGTCCGCTGAATAATCCGCAAAAATTGCAGTTCAATGCTTACACCGACAACAACCCGAAAGGGTTTGGTTTATTGCAACTGGATCGTGACTTCTCCCATTATCAGGACATTATGGGCTGGTATAACAAACGCCCAAGTCTGTGGGTGGAACCGCGTAACAAGTGGGGTAAGGGCACCATCGGCCTGATGGAAATCCCAACAACGGGCGAAACGCTGGATAACATTGTCTGCTTCTGGCAGCCAGAAAAAGCTGTAAAAGCAG  >  NC_000913/1497794‑1498062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑