Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	234,047	A→G	S246P (TCA→CCA)	gloB ←	hydroxyacylglutathione hydrolase GloB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	234,047	0	A	G	100.0%	129.2 / NA	37	S246P (TCA→CCA)	gloB	hydroxyacylglutathione hydrolase GloB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/14);  total (23/14)

ACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTAAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTAT  >  NC_000913/233911‑234182                                                                                                                                         |                                                                                                                                        aCCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCt                                                                                                                                      <  1:888130/140‑1 (MQ=255)      cGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAAcc                                                                                                                                 >  1:684519/1‑140 (MQ=255)                  agGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGct                                                                                                                     >  2:251610/1‑140 (MQ=255)                  agGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGct                                                                                                                     >  1:676024/1‑140 (MQ=255)                   ggCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACg                                                                                                                       >  2:270622/1‑137 (MQ=255)                   ggCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACg                                                                                                                       <  1:270622/137‑1 (MQ=255)                     cGAGTAATATCGCTTTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCtt                                                                                                                  <  1:665484/140‑1 (MQ=255)                       aGTAATATCGCTTTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCa                                                                                                                >  2:994093/1‑140 (MQ=255)                       aGTAATATCGCTTTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCa                                                                                                                >  2:433920/1‑140 (MQ=255)                       aGTAATATCGCTTTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCa                                                                                                                >  1:940234/1‑140 (MQ=255)                       aGTAATATCGCTTTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCa                                                                                                                >  1:699852/1‑140 (MQ=255)                                     tGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGgt                                                                                                             >  1:180131/1‑129 (MQ=255)                                     tGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGgt                                                                                                             <  2:180131/129‑1 (MQ=255)                                            aTGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTttct                                                                                           >  2:275065/1‑140 (MQ=255)                                                 tgtgtCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAGAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTcatt                                                                                      >  1:210758/1‑140 (MQ=255)                                                 tgtgtCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTcatt                                                                                      <  2:676024/140‑1 (MQ=255)                                                 tgtgtCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTcatt                                                                                      >  2:263681/1‑140 (MQ=255)                                                   tgtCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTcattaa                                                                                    <  2:732812/140‑1 (MQ=255)                                                    gtCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTcattaat                                                                                   <  1:85345/140‑1 (MQ=255)                                                        aTAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTcattaattaca                                                                               <  1:275065/140‑1 (MQ=255)                                                         tAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTcattaattacat                                                                              >  2:629803/1‑140 (MQ=255)                                                           gTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTcattaattacatta                                                                            >  2:911703/1‑140 (MQ=255)                                                            ttGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTcattaattacattaa                                                                           >  2:697368/1‑140 (MQ=255)                                                             tGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTcattaattacattaat                                                                          <  2:699852/140‑1 (MQ=255)                                                                          acCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTACATTAATTACATTAATTAAATCAATATCt                                                             >  2:796591/1‑140 (MQ=255)                                                                              aTCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCg                                                         >  2:721043/1‑140 (MQ=255)                                                                                       gCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTaaaa                                                <  1:276900/140‑1 (MQ=255)                                                                                        cGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTaaaaa                                               >  1:193956/1‑140 (MQ=255)                                                                                             ggAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTAAAAAAACAt                                          >  1:96289/1‑140 (MQ=255)                                                                                                                 tttATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTAAAAAAACATTAATTTGCCGCTCATTTTTc                      <  1:994093/140‑1 (MQ=255)                                                                                                                  ttATCAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTAAAAAAACATTAATTTGCCGCTCATTTTTCa                     <  1:433920/140‑1 (MQ=255)                                                                                                                      cAGAACCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTAAAAAAACATTAATTTGCCGCTCATTTTTCAGAAt                 <  2:690277/140‑1 (MQ=255)                                                                                                                         aaCCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTAAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTAc              >  1:34380/1‑140 (MQ=255)                                                                                                                          aCCTATCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTAAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACg             >  1:783501/1‑140 (MQ=255)                                                                                                                              aTCTTTCTTTGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTAAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTa         >  1:179019/1‑140 (MQ=255)                                                                                                                                   tctttGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTAAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGtt    >  2:610243/1‑140 (MQ=255)                                                                                                                                     tttGGCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTAAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTAt  <  1:263681/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        ACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTAAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTAT  >  NC_000913/233911‑234182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑