Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,611,174	A→G	A227A (GCT→GCC)	dgcF ←	putative diguanylate cyclase DgcF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,611,174	0	A	G	100.0%	292.3 / NA	81	A227A (GCT→GCC)	dgcF	putative diguanylate cyclase DgcF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/45);  total (36/45)

CGACCACTACCGACGCCAATACTTACCGTGAGATAAAGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGT  >  NC_000913/1611037‑1611307                                                                                                                                          |                                                                                                                                      cgACCACTACCGACGCCAATACTTACCGTGAGATAAAGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGc                                                                                                                                     >  2:643780/1‑140 (MQ=255)       cTACCGACGCCAATACTTACCGTGAGATAAAGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAAttc                                                                                                                               >  1:720256/1‑140 (MQ=255)               gCCAATACTTACCGTGAGATAAAGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTcgccgc                                                                                                                       >  1:935920/1‑140 (MQ=255)                 cAATACTTACCGTGAGATAAAGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACGGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGccc                                                                                                                     >  1:532740/1‑140 (MQ=255)                  aaTACTTACCGTGAGATAAAGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCa                                                                                                                    <  2:533600/140‑1 (MQ=255)                    tACTTACCGTGAGATAAAGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCAtt                                                                                                                  >  1:374170/1‑140 (MQ=255)                      cTTACCGTGAGATAAAGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTcg                                                                                                                <  2:842159/140‑1 (MQ=255)                         aCCGTGAGATAAAGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTcgccgc                                                                                                                       >  1:197778/1‑130 (MQ=255)                         aCCGTGAGATAAAGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTcgccgc                                                                                                                       <  2:197778/130‑1 (MQ=255)                         aCCGTGAGATAAAGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCgcgc                                                                                                             >  2:981864/1‑140 (MQ=255)                           cGTGAGATAAAGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGcca                                                                                                           >  1:120155/1‑140 (MQ=255)                             tGAGATAAAGCGTTTTTTGTTGCCAGGTGAATGTTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGccacc                                                                                                         <  2:997607/140‑1 (MQ=255)                             tGAGATAAAGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGccacc                                                                                                         <  1:1993/140‑1 (MQ=255)                              gagaTAAAGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGccacca                                                                                                        >  1:308763/1‑140 (MQ=255)                              gagaTAAAGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGccac                                                                                                          <  1:595648/138‑1 (MQ=255)                              gagaTAAAGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGccac                                                                                                          >  2:595648/1‑138 (MQ=255)                              gagaTAAAGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCC‑TTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAg                                                                                                       >  1:477775/1‑140 (MQ=255)                                   aaaGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGcccc                                                                                                   <  2:308763/140‑1 (MQ=255)                                     aGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCtt                                                                                                 >  2:239350/1‑140 (MQ=255)                                     aGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCtt                                                                                                 >  1:275500/1‑140 (MQ=255)                                       cGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTAt                                                                                               <  2:935920/140‑1 (MQ=255)                                         ttttttGTTGCCAGGTGAATGGTTGCAGTTCAACGCC‑TTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCAc                                                                                            <  2:477775/140‑1 (MQ=255)                                           ttttGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCAcc                                                                                           >  1:285090/1‑140 (MQ=255)                                           ttttGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCAcc                                                                                           >  2:20508/1‑140 (MQ=255)                                            tttGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCg                                                                                          >  1:378099/1‑140 (MQ=255)                                               gttgCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACa                                                                                       <  1:208123/140‑1 (MQ=255)                                                 tgCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAAt                                                                                     <  1:239350/140‑1 (MQ=255)                                                          aaTGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAat                                                                            >  2:162305/1‑140 (MQ=255)                                                          aaTGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAat                                                                            >  2:859276/1‑140 (MQ=255)                                                          aaTGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAat                                                                            >  2:286234/1‑140 (MQ=255)                                                              gTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGc                                                                        <  1:981864/140‑1 (MQ=255)                                                                  cAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCggg                                                                    <  1:629737/140‑1 (MQ=255)                                                                  cAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCggg                                                                    >  2:567574/1‑140 (MQ=255)                                                                       cAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCATCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAac                                                               >  1:524259/1‑140 (MQ=255)                                                                       cAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAac                                                               <  1:90777/140‑1 (MQ=255)                                                                        aaCGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAaca                                                              >  1:416197/1‑140 (MQ=255)                                                                          cGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACg                                                            <  2:374170/140‑1 (MQ=255)                                                                          cGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACg                                                            <  1:463742/140‑1 (MQ=255)                                                                          cGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACg                                                            <  2:720256/140‑1 (MQ=255)                                                                            ccTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCt                                                          <  2:285090/140‑1 (MQ=255)                                                                            ccTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCt                                                          <  1:859276/140‑1 (MQ=255)                                                                            ccTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCt                                                          <  1:142660/140‑1 (MQ=255)                                                                             cTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCtt                                                         <  2:126396/140‑1 (MQ=255)                                                                               ttACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTaa                                                       >  1:914086/1‑140 (MQ=255)                                                                                  cGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAacac                                                    <  2:275500/140‑1 (MQ=255)                                                                                         tttCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTa                                                <  2:734706/137‑1 (MQ=255)                                                                                         tttCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTa                                                >  1:734706/1‑137 (MQ=255)                                                                                         tttCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCg                                             <  2:120155/140‑1 (MQ=255)                                                                                         tttCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCg                                             <  1:395840/140‑1 (MQ=255)                                                                                            ccGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCca                                          <  1:286234/140‑1 (MQ=255)                                                                                            ccGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAAAATGCCGGGCAAACACGCTTAACACTCTATCGCca                                          <  1:628888/140‑1 (MQ=255)                                                                                             cGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCcac                                         >  1:781233/1‑140 (MQ=255)                                                                                              gCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCcaca                                        <  2:914086/140‑1 (MQ=255)                                                                                                cATTAGCAGACCATCGACAGGATTCATCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACAtt                                      <  2:524259/140‑1 (MQ=255)                                                                                                 aTTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCTGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTc                                     <  2:908137/140‑1 (MQ=255)                                                                                                 aTTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTc                                     <  2:416197/140‑1 (MQ=255)                                                                                                        gACCATCGACGGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCa                              <  2:532740/140‑1 (MQ=255)                                                                                                        gACCATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCa                              <  1:567574/140‑1 (MQ=255)                                                                                                           cATCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAg                           >  1:923981/1‑140 (MQ=255)                                                                                                             tCGACAGGATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGtt                         <  2:378099/140‑1 (MQ=255)                                                                                                             tCGACAGGATTCACCAACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGtt                         <  1:779510/140‑1 (MQ=255)                                                                                                             tCGACAGGATTCACCAACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGtt                         >  2:779510/1‑140 (MQ=255)                                                                                                                    gATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACTGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCa                              <  2:330521/128‑1 (MQ=255)                                                                                                                    gATTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACTGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCa                              >  1:330521/1‑128 (MQ=255)                                                                                                                     aTTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTaa                 >  1:13290/1‑140 (MQ=255)                                                                                                                     aTTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTaa                 <  1:50256/140‑1 (MQ=255)                                                                                                                     aTTCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTaa                 >  2:456966/1‑140 (MQ=255)                                                                                                                      ttCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAAt                <  1:643083/140‑1 (MQ=255)                                                                                                                      ttCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAAt                <  1:909839/140‑1 (MQ=255)                                                                                                                      ttCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAAt                <  1:20508/140‑1 (MQ=255)                                                                                                                       tCACCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATg               >  2:121474/1‑140 (MQ=255)                                                                                                                         aCCGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCt             >  2:602238/1‑140 (MQ=255)                                                                                                                           cGACGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCtgt           >  2:676492/1‑138 (MQ=255)                                                                                                                              cGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAAt                <  1:866308/132‑1 (MQ=255)                                                                                                                              cGGCACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAAt                >  2:866308/1‑132 (MQ=255)                                                                                                                                 cACTGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAAt     >  1:906531/1‑140 (MQ=255)                                                                                                                                    tGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGt  <  1:456966/140‑1 (MQ=255)                                                                                                                                    tGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGt  <  1:602238/140‑1 (MQ=255)                                                                                                                                    tGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGt  >  1:272020/1‑140 (MQ=255)                                                                                                                                    tGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGt  <  2:906531/140‑1 (MQ=255)                                                                                                                                    tGCAACGGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGt  <  1:162305/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      CGACCACTACCGACGCCAATACTTACCGTGAGATAAAGCGTTTTTTGTTGCCAGGTGAATGGTTGCAGTTCAACGCCTTTACGGATTTTTTCCGCCATTAGCAGACCATCGACAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGT  >  NC_000913/1611037‑1611307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑