Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,631,586	T→C	T234A (ACT→GCT)	ydfJ ←	putative transporter YdfJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,631,586	0	T	C	100.0%	214.3 / NA	61	T234A (ACT→GCT)	ydfJ	putative transporter YdfJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/25);  total (36/25)

GCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGCCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACC  >  NC_000913/1631452‑1631722                                                                                                                                       |                                                                                                                                         gCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGCCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGaa                                                                                                                                     >  1:820054/1‑140 (MQ=255)    aCCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGCCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTa                                                                                                                                  >  2:990547/1‑140 (MQ=255)     ccAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGCCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTaa                                                                                                                                 >  1:433629/1‑140 (MQ=255)     ccAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGCCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTaa                                                                                                                                 >  1:95169/1‑140 (MQ=255)          tttATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGCCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCgg                                                                                                                            <  2:273631/140‑1 (MQ=255)                   tAACCAACCAAGGAACGGAATGGTCATAAAGCCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCg                                                                                                                   >  1:680102/1‑140 (MQ=255)                                aaCGGAATGGTCATAAAGCCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACg                                                                                                      >  1:1000695/1‑140 (MQ=255)                                aaCGGAATGGTCATAAAGCCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACg                                                                                                      <  2:433629/140‑1 (MQ=255)                                 aCGGAATGGTCATAAAGCCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGc                                                                                                     >  1:607175/1‑140 (MQ=255)                                         gTCATAAAGCCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGt                                                                                             >  1:325198/1‑140 (MQ=255)                                             tAAAGCCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTgcc                                                                                         <  1:454406/140‑1 (MQ=255)                                               aaGCCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTgccag                                                                                       >  1:161937/1‑140 (MQ=255)                                                  ccGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTgccagcca                                                                                    <  2:607175/140‑1 (MQ=255)                                                     agaATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGaa                                                                                 <  2:325198/140‑1 (MQ=255)                                                     agaATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGaa                                                                                 >  1:275384/1‑140 (MQ=255)                                                      gaATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTTACCAAAACGCAGCCCTGTTGCCAGCCAGAAg                                                                                >  1:959777/1‑140 (MQ=255)                                                         tCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGAt                                                                             <  1:505876/140‑1 (MQ=255)                                                          cGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGAtt                                                                            <  2:820054/140‑1 (MQ=255)                                                                  tcatcaATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTgg                                                                    <  1:681472/140‑1 (MQ=255)                                                                     tcaATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAAc                                                                 <  2:95169/140‑1 (MQ=255)                                                                     tcaATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAAc                                                                 <  2:161937/140‑1 (MQ=255)                                                                         tGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCTGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGAt                                                                             >  2:987253/1‑124 (MQ=255)                                                                         tGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCTGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGAt                                                                             <  1:987253/124‑1 (MQ=255)                                                                           cATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTa                                                           >  2:624588/1‑140 (MQ=255)                                                                           cATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTa                                                           >  1:707454/1‑140 (MQ=255)                                                                           cATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTa                                                           >  2:462175/1‑140 (MQ=255)                                                                           cATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTa                                                           >  2:191946/1‑140 (MQ=255)                                                                           cATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTa                                                           >  1:913707/1‑140 (MQ=255)                                                                              cTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGTAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACca                                                        <  2:282045/140‑1 (MQ=255)                                                                              cTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACca                                                        >  2:558932/1‑140 (MQ=255)                                                                               tGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACcag                                                       <  2:913707/140‑1 (MQ=255)                                                                                        tGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAACCGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAgcaggtgcag                                              >  1:669072/1‑140 (MQ=255)                                                                                          cTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAgcaggtgcaggt                                            >  1:576277/1‑140 (MQ=255)                                                                                            ttGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGttt                                          <  2:275384/140‑1 (MQ=255)                                                                                                  aaCAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTTACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGt                                    <  2:959777/140‑1 (MQ=255)                                                                                                  aaCAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGt                                    <  2:670024/140‑1 (MQ=255)                                                                                                    cAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCg                                  <  1:29779/140‑1 (MQ=255)                                                                                                    cAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCg                                  <  1:191946/140‑1 (MQ=255)                                                                                                     aaTAACGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCgg                                 <  2:609227/140‑1 (MQ=255)                                                                                                         aCGTCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCggttgg                             >  1:889279/1‑140 (MQ=255)                                                                                                            tCTGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTa                          <  1:624588/140‑1 (MQ=255)                                                                                                              tGCACTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACt                        >  1:765467/1‑140 (MQ=255)                                                                                                                 aCTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTc                     >  1:863249/1‑140 (MQ=255)                                                                                                                 aCTAAATAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTc                     >  1:297543/1‑140 (MQ=255)                                                                                                                    aaaTAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAACCGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGtt                  <  2:669072/140‑1 (MQ=255)                                                                                                                     aaTAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTa                 >  1:901119/1‑140 (MQ=255)                                                                                                                     aaTAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTa                 >  1:970346/1‑140 (MQ=255)                                                                                                                       tAGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAAc               >  1:78045/1‑140 (MQ=255)                                                                                                                        aGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAAcc              <  2:680102/140‑1 (MQ=255)                                                                                                                        aGCCTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAAcc              <  2:889279/140‑1 (MQ=255)                                                                                                                          ccTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAAcc              <  2:309347/138‑1 (MQ=255)                                                                                                                          ccTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAAcc              >  1:309347/1‑138 (MQ=255)                                                                                                                          ccTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCtt            >  1:102890/1‑140 (MQ=255)                                                                                                                          ccTGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCtt            >  1:961938/1‑140 (MQ=255)                                                                                                                            tGCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTtct          >  1:157587/1‑140 (MQ=255)                                                                                                                             gCAAGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTtctc         >  1:501166/1‑140 (MQ=255)                                                                                                                               aaGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAAGGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACGGTCGTTAACCTTCTCaa       >  2:889846/1‑140 (MQ=255)                                                                                                                               aaGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCaa       >  1:950302/1‑140 (MQ=255)                                                                                                                               aaGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCaa       >  1:307160/1‑140 (MQ=255)                                                                                                                               aaGGAAAGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCaa       >  1:240511/1‑140 (MQ=255)                                                                                                                                    aaGCCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGAcc  <  2:78045/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         GCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGCCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACC  >  NC_000913/1631452‑1631722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑