Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,695,409	T→C	Q221Q (CAA→CAG)	uidA ←	beta‑glucuronidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,695,409	0	T	C	100.0%	129.1 / NA	37	Q221Q (CAA→CAG)	uidA	beta‑glucuronidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/17);  total (20/17)

AGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAG  >  NC_000913/1695279‑1695544                                                                                                                                   |                                                                                                                                        aGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCa                                                                                                                                <  2:240636/140‑1 (MQ=255)      tCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACg                                                                                                                           <  1:447184/140‑1 (MQ=255)             ctgtctgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCa                                                                                                                    <  1:543999/140‑1 (MQ=255)             ctgtctgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGGGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCa                                                                                                                    >  2:209619/1‑140 (MQ=255)                tctgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACg                                                                                                                           >  2:280693/1‑130 (MQ=255)                tctgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACg                                                                                                                           <  1:280693/130‑1 (MQ=255)                tctgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACg                                                                                                                 >  1:76157/1‑140 (MQ=255)                tctgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACg                                                                                                                 >  2:72500/1‑140 (MQ=255)                  tgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCt                                                                                                               <  1:886074/140‑1 (MQ=255)                       tttGGCTGTGACGCACAGTTCATAGACATAACCTTCACCCAGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACTACCTGCTGATCCGCATCACGCAGTTCAACGCTGACAt                                                                                                          <  2:883587/140‑1 (MQ=255)                          ggCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACAt                                                                                                          >  1:570722/1‑137 (MQ=255)                          ggCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACAt                                                                                                          <  2:570722/137‑1 (MQ=255)                          ggCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCAc                                                                                                       >  1:131877/1‑140 (MQ=255)                              gtgACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCAtt                                                                                                   >  2:285667/1‑140 (MQ=255)                                 aCGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGc                                                                                                <  1:72500/140‑1 (MQ=255)                                           cATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGc                                                                                      <  1:731938/140‑1 (MQ=255)                                           cATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGc                                                                                      <  1:285667/140‑1 (MQ=255)                                           cATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGc                                                                                      <  1:276908/140‑1 (MQ=255)                                             tAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCa                                                                                    >  1:500066/1‑140 (MQ=255)                                             tAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCa                                                                                    <  1:86259/140‑1 (MQ=255)                                              agagATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCTGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAg                                                                                   >  1:409608/1‑140 (MQ=255)                                                 gaTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCa                                                                                >  1:295009/1‑140 (MQ=255)                                                   tAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAAc                                                                              >  1:177790/1‑140 (MQ=255)                                                      ccTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGa                                                                           <  2:76157/140‑1 (MQ=255)                                                          cACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGAcgcg                                                                       >  2:621405/1‑140 (MQ=255)                                                                gTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTa                                                                 >  2:923802/1‑140 (MQ=255)                                                                           tGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTgcgc                                                      >  1:867706/1‑140 (MQ=255)                                                                               gATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACa                                                  <  2:645729/140‑1 (MQ=255)                                                                                            cAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACgg                                     >  1:235403/1‑140 (MQ=255)                                                                                                    cGCTAGTGCCTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCg                             >  2:618771/1‑140 (MQ=255)                                                                                                             cTTGTCCAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAg                    >  1:418059/1‑140 (MQ=255)                                                                                                                  ccAGTTGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGtt               <  2:500066/140‑1 (MQ=255)                                                                                                                       tGCAACCACCTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCg          <  1:923802/140‑1 (MQ=255)                                                                                                                           accaccTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCgtggt      >  2:740629/1‑140 (MQ=255)                                                                                                                           accaccTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCg          <  2:153000/136‑1 (MQ=255)                                                                                                                           accaccTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCg          >  1:153000/1‑136 (MQ=255)                                                                                                                               ccTGCTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTag  >  1:63851/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        AGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAG  >  NC_000913/1695279‑1695544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑