Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,717,792 T→C K72K (AAA→AAG)  pdxH ← pyridoxal 5‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,717,7920TC100.0% 149.5 / NA 43K72K (AAA→AAG) pdxHpyridoxal 5‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/26);  total (17/26)

ACCGATCACCATCACCTGGCGCTCAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGA  >  NC_000913/1717660‑1717918
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aCCGATCACCATCACCTGGCGCTCAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTa                                                                                                                         <  1:785286/140‑1 (MQ=255)
    atcaccatcaccTGGCGCTCAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAAc                                                                                                                     >  2:84047/1‑140 (MQ=255)
    atcaccatcaccTGGCGCTCAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAAc                                                                                                                     >  2:101211/1‑140 (MQ=255)
       accatcaccTGGCGCTCAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGAt                                                                                                                  <  1:159483/140‑1 (MQ=255)
        ccatcaccTGGCGCTCAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATg                                                                                                                 <  1:84047/140‑1 (MQ=255)
             accTGGCGCTCAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTg                                                                                                            >  1:113643/1‑140 (MQ=255)
              ccTGGCGCTCAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGa                                                                                                           >  1:198232/1‑140 (MQ=255)
                   cgcTCAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAgg                                                                                                      <  1:86345/140‑1 (MQ=255)
                   cgcTCAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAgg                                                                                                      <  2:642999/140‑1 (MQ=255)
                   cgcTCAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAgg                                                                                                      >  2:570102/1‑140 (MQ=255)
                     cTCAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCt                                                                                                    >  2:510708/1‑140 (MQ=255)
                       cAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGAt                                                                                                          >  2:223837/1‑132 (MQ=255)
                       cAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGAt                                                                                                          <  1:223837/132‑1 (MQ=255)
                       cAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGa                                                                                                  >  2:55177/1‑140 (MQ=255)
                       cAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGa                                                                                                  >  1:957886/1‑140 (MQ=255)
                         aGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGAcc                                                                                                <  2:113643/140‑1 (MQ=255)
                             tATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGt                                                                                            <  2:669181/140‑1 (MQ=255)
                             tATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGt                                                                                            <  2:504450/140‑1 (MQ=255)
                                 ccACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCAt                                                                                        <  2:957886/140‑1 (MQ=255)
                                  cACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATc                                                                                       <  2:216105/140‑1 (MQ=255)
                                    cGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCa                                                                                     >  1:787830/1‑140 (MQ=255)
                                    cGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCa                                                                                     <  2:198232/140‑1 (MQ=255)
                                              agGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGac                                                                           <  2:369718/140‑1 (MQ=255)
                                                   aaCGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGAccacca                                                                      <  1:283659/140‑1 (MQ=255)
                                                         cgGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCgg                                                                <  1:55177/140‑1 (MQ=255)
                                                         cgGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCgg                                                                <  1:257483/140‑1 (MQ=255)
                                                           gATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTa                                                              <  1:510708/140‑1 (MQ=255)
                                                              tattTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAggg                                                           >  1:653767/1‑140 (MQ=255)
                                                                          gATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCgg                                                                <  1:782903/123‑1 (MQ=255)
                                                                          gATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCgg                                                                >  2:782903/1‑123 (MQ=255)
                                                                                cTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAGCGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTca                                         <  1:570102/140‑1 (MQ=255)
                                                                                cTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTca                                         <  1:751242/140‑1 (MQ=255)
                                                                                cTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTca                                         >  2:686382/1‑140 (MQ=255)
                                                                                       gCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGCCt                                  <  1:123355/140‑1 (MQ=255)
                                                                                           ccGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGCCTgaga                              >  1:200630/1‑140 (MQ=255)
                                                                                               ggTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGc                          <  1:101211/140‑1 (MQ=255)
                                                                                                  tGGTGTAAAACACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCaa                       <  1:686382/140‑1 (MQ=255)
                                                                                                          aaCCCCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCaaa               <  1:92887/140‑1 (MQ=255)
                                                                                                          aaCACCATGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCaaa               <  1:201912/140‑1 (MQ=255)
                                                                                                                 tGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCaaaa              <  2:85038/134‑1 (MQ=255)
                                                                                                                 tGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCaaaa              >  1:85038/1‑134 (MQ=255)
                                                                                                                 tGCCTTTTTCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGtt        >  1:99097/1‑140 (MQ=255)
                                                                                                                       tttCGTCGTAATGCTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGa  >  2:632940/1‑140 (MQ=255)
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ACCGATCACCATCACCTGGCGCTCAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTTACGGCTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGA  >  NC_000913/1717660‑1717918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑