Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,735,220	T→C	R262R (CGT→CGC)	mepH →	peptidoglycan DD‑endopeptidase MepH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,735,220	0	T	C	100.0%	234.3 / NA	66	R262R (CGT→CGC)	mepH	peptidoglycan DD‑endopeptidase MepH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/33);  total (33/33)

CGCGGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGCACAATAAGGCTATTGTACGTATGCAAA  >  NC_000913/1735089‑1735355                                                                                                                                    |                                                                                                                                        cgcgGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAAt                                                                                                                                 >  2:957618/1‑140 (MQ=255)  gcgGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATg                                                                                                                                <  1:82340/140‑1 (MQ=255)    ggCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGAc                                                                                                                              <  2:640354/140‑1 (MQ=255)    ggCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGAc                                                                                                                              >  1:422216/1‑140 (MQ=255)      cacaGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGAccc                                                                                                                            <  1:305382/140‑1 (MQ=255)                   gTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCg                                                                                                               >  1:966811/1‑140 (MQ=255)                     cGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCt                                                                                                             >  2:209673/1‑140 (MQ=255)                     cGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCt                                                                                                             >  1:763775/1‑140 (MQ=255)                        cGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTaaa                                                                                                          >  1:352535/1‑140 (MQ=255)                           gtATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTATCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACt                                                                                                       <  2:203586/140‑1 (MQ=255)                                tCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACCCTTCGCTAAAACTTTTcc                                                                                                  <  2:478159/140‑1 (MQ=255)                                 cggcaacggcaaATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTAccc                                                                                                 >  1:379418/1‑140 (MQ=255)                                  ggcaacggcaaATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTAcc                                                                                                  >  2:266134/1‑138 (MQ=255)                                  ggcaacggcaaATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTAcc                                                                                                  <  1:266134/138‑1 (MQ=255)                                  ggcaacggcaaATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCt                                                                                                <  2:352535/140‑1 (MQ=255)                                     aacggcaaATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCtgtt                                                                                             <  2:233336/140‑1 (MQ=255)                                      acggcaaATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTa                                                                                                    >  2:309663/1‑132 (MQ=255)                                      acggcaaATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTa                                                                                                    <  1:309663/132‑1 (MQ=255)                                         gcaaATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTa                                                                                         >  1:915455/1‑140 (MQ=255)                                               ttATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCaa                                                                                   <  2:966811/140‑1 (MQ=255)                                                tATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAAc                                                                                  <  2:763775/140‑1 (MQ=255)                                                 aTTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTAc                                                                                                   <  2:946327/122‑1 (MQ=255)                                                 aTTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTAc                                                                                                   >  1:946327/1‑122 (MQ=255)                                                  ttCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACCACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAg                                                                                >  2:207307/1‑140 (MQ=255)                                                   tCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAgg                                                                               <  1:970523/140‑1 (MQ=255)                                                     aGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGt                                                                             <  1:856823/140‑1 (MQ=255)                                                      gTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTa                                                                            >  2:581266/1‑140 (MQ=255)                                                         aCCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTa                                                                                         <  2:399027/124‑1 (MQ=255)                                                         aCCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTa                                                                                         >  1:399027/1‑124 (MQ=255)                                                             cgcACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCAt                                                                     >  2:660144/1‑140 (MQ=255)                                                               cacaGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCt                                                                   >  1:689989/1‑140 (MQ=255)                                                                    gTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGt                                                              >  2:121043/1‑140 (MQ=255)                                                                      cAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGtct                                                            <  1:433331/140‑1 (MQ=255)                                                                       aGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGtctc                                                           >  1:574807/1‑140 (MQ=255)                                                                        ggAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCa                                                          <  2:753092/140‑1 (MQ=255)                                                                        ggAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCa                                                          >  1:753092/1‑140 (MQ=255)                                                                            aTTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCa                                                                      >  1:434949/1‑124 (MQ=255)                                                                            aTTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCa                                                                      <  2:434949/124‑1 (MQ=255)                                                                            aTTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCtt                                                      >  1:180713/1‑140 (MQ=255)                                                                                    cACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTg                                              <  2:379418/140‑1 (MQ=255)                                                                                    cACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTg                                              <  2:812705/140‑1 (MQ=255)                                                                                    cACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTg                                              <  1:660144/140‑1 (MQ=255)                                                                                         ctctcAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGc                                             <  1:740400/136‑1 (MQ=255)                                                                                         ctctcAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGc                                             >  2:740400/1‑136 (MQ=255)                                                                                              aGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCGTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATc                                    <  1:636634/140‑1 (MQ=255)                                                                                                 gAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCAt                                 >  2:562052/1‑140 (MQ=255)                                                                                                    gACTACTGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACg                              <  2:411381/140‑1 (MQ=255)                                                                                                     actactGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTg                                              >  2:150867/1‑123 (MQ=255)                                                                                                     actactGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTg                                              <  1:150867/123‑1 (MQ=255)                                                                                                      ctactGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGca                            >  1:679003/1‑140 (MQ=255)                                                                                                         ctGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGCACaa                         <  1:957618/140‑1 (MQ=255)                                                                                                         ctGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGCACaa                         <  1:207307/140‑1 (MQ=255)                                                                                                          tGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGCACAAt                        >  2:333802/1‑140 (MQ=255)                                                                                                          tGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGCACAAt                        <  1:887080/140‑1 (MQ=255)                                                                                                          tGGCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGCACAAt                        >  2:904110/1‑140 (MQ=255)                                                                                                           ggCAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCGCC‑TTTAATTTGCTACCCTATCCATACGCACAATaa                      <  1:581266/140‑1 (MQ=255)                                                                                                             cAGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGCACAATAAg                     <  2:574807/140‑1 (MQ=255)                                                                                                              aGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGCACAATAAgg                    <  2:233525/140‑1 (MQ=255)                                                                                                              aGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGCACAATAAgg                    <  1:904110/140‑1 (MQ=255)                                                                                                              aGCGCCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGCGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGCACAATAAgg                    <  2:689989/140‑1 (MQ=255)                                                                                                                 gcCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGCACAATAAGGCTa                 >  2:970158/1‑140 (MQ=255)                                                                                                                 gcCACTATGTTGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGCACAATAAGGCTa                 >  2:854360/1‑140 (MQ=255)                                                                                                                           tGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGCACAATaa                      >  2:625269/1‑125 (MQ=255)                                                                                                                           tGGCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGCACAATaa                      <  1:625269/125‑1 (MQ=255)                                                                                                                            ggCGCTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGCACAATAAGGCTATTGTACGTATg      >  1:203249/1‑140 (MQ=255)                                                                                                                             gcgcTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGCACAATAAGGCTATTGTACGTATGc     >  1:177830/1‑140 (MQ=255)                                                                                                                                cTCGCCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGCACAATAAGGCTATTGTACGTATGCaaa  >  1:184615/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        CGCGGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCCTATCCATACGCACAATAAGGCTATTGTACGTATGCAAA  >  NC_000913/1735089‑1735355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑