Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,784,424	T→C	V483A (GTT→GCT)	fadK →	short chain acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,784,424	0	T	C	100.0%	178.4 / NA	51	V483A (GTT→GCT)	fadK	short chain acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/27);  total (24/27)

TGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGAA  >  NC_000913/1784292‑1784560                                                                                                                                     |                                                                                                                                         tGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATg                                                                                                                                   >  1:188009/1‑140 (MQ=255)      gAGGCTGGCTATATAAAAATTACCGGACGC‑AAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGAt                                                                                                                             <  2:935008/140‑1 (MQ=255)         gctggctATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCAGTGTGGCTGCAATGTCCGATGa                                                                                                                           >  1:706400/1‑140 (MQ=255)               tatataAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGttt                                                                                                                     <  1:527032/140‑1 (MQ=255)                 tataAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAg                                                                                                                   >  1:20619/1‑140 (MQ=255)                   taAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGt                                                                                                                 <  1:901932/140‑1 (MQ=255)                     aaaaTTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATg                                                                                                                            <  2:376663/127‑1 (MQ=255)                     aaaaTTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATg                                                                                                                            >  1:376663/1‑127 (MQ=255)                           aCCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATc                                                                                                         >  1:180676/1‑140 (MQ=255)                           aCCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATc                                                                                                         >  1:236815/1‑140 (MQ=255)                            ccGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCa                                                                                                        <  1:191173/140‑1 (MQ=255)                            ccGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCa                                                                                                        <  2:394977/140‑1 (MQ=255)                            ccGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCa                                                                                                        <  1:394155/140‑1 (MQ=255)                             cGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCa                                                                                                        <  2:943217/139‑1 (MQ=255)                             cGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCa                                                                                                        >  1:943217/1‑139 (MQ=255)                              ggACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATg                                                                                                      <  2:240637/140‑1 (MQ=255)                                  gCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATc                                                                                                         >  2:859562/1‑133 (MQ=255)                                  gCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATc                                                                                                         <  1:859562/133‑1 (MQ=255)                                              ttattGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTg                                                                                      >  2:254197/1‑140 (MQ=255)                                                  tGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAg                                                                                  >  1:425645/1‑140 (MQ=255)                                                    tCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAgcg                                                                                >  1:496437/1‑140 (MQ=255)                                                       gcggcggcgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATTATGCGCTTATGTCGTGCTGAAAGcgccg                                                                             <  2:429021/140‑1 (MQ=255)                                                       gcggcggcgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAgcg                                                                                <  2:811067/137‑1 (MQ=255)                                                       gcggcggcgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAgcg                                                                                >  1:811067/1‑137 (MQ=255)                                                        cggcggcgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGcgccgc                                                                            >  2:504131/1‑140 (MQ=255)                                                         ggcggcgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGca                                                                           <  2:496437/140‑1 (MQ=255)                                                         ggcggcgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGca                                                                           >  2:870798/1‑140 (MQ=255)                                                            ggcgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGcatca                                                                        <  2:180676/140‑1 (MQ=255)                                                              cgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCAtcatt                                                                      >  2:275387/1‑140 (MQ=255)                                                              cgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCAtcatt                                                                      >  1:85504/1‑140 (MQ=255)                                                                  aaTATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATcattcatt                                                                  <  2:178403/140‑1 (MQ=255)                                                                    tatTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTAt                                                                <  1:504131/140‑1 (MQ=255)                                                                       tAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGc                                                             >  1:497193/1‑140 (MQ=255)                                                                             ccGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAag                                                       >  1:737885/1‑140 (MQ=255)                                                                              cGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCt                                                            >  2:856441/1‑134 (MQ=255)                                                                              cGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCt                                                            <  1:856441/134‑1 (MQ=255)                                                                                  aaGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTa                                                  <  2:497193/140‑1 (MQ=255)                                                                                       gAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGc                                             >  1:198377/1‑140 (MQ=255)                                                                                             atTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCtttttt                                       <  2:737885/140‑1 (MQ=255)                                                                                              ttttATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCttttttt                                      <  2:188009/140‑1 (MQ=255)                                                                                                        gcaTCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAAc                            >  1:501303/1‑140 (MQ=255)                                                                                                           tCCTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGCGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACggg                         >  1:108887/1‑140 (MQ=255)                                                                                                             cTAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTc                       >  1:869860/1‑140 (MQ=255)                                                                                                              tAAAATTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCg                      <  2:236815/140‑1 (MQ=255)                                                                                                               aaaaTTCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGc                     <  1:275387/140‑1 (MQ=255)                                                                                                                   ttCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCaaaa                 <  1:348323/140‑1 (MQ=255)                                                                                                                   ttCACGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCACCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCaaaa                 <  1:254197/140‑1 (MQ=255)                                                                                                                      aCGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCg                      >  1:804888/1‑132 (MQ=255)                                                                                                                      aCGATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCg                      <  2:804888/132‑1 (MQ=255)                                                                                                                        gATGCCTGTGTGGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAaaatataa            <  2:501303/140‑1 (MQ=255)                                                                                                                                  tgGCTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGaa  <  2:877118/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         TGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGAA  >  NC_000913/1784292‑1784560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑