Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,850,562	T→C	F576L (TTC→CTC)	sppA →	protease IV, a signal peptide peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,850,562	0	T	C	100.0%	107.1 / NA	31	F576L (TTC→CTC)	sppA	protease IV, a signal peptide peptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/13);  total (18/13)

GTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGTTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCT  >  NC_000913/1850433‑1850697                                                                                                                                  |                                                                                                                                        gTCGCCAAAGCAGCAGCGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCAt                                                                                                                               <  2:896080/140‑1 (MQ=255)      cAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTAc                                                                                                                          <  2:263944/140‑1 (MQ=255)              cagAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCg                                                                                                                  <  1:802816/140‑1 (MQ=255)              cagAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCg                                                                                                                  >  2:802816/1‑140 (MQ=255)                        aaaaGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCgg                                                                                                        >  1:593013/1‑140 (MQ=255)                                       gCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTa                                                                                         >  2:651141/1‑140 (MQ=255)                                             ggAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAaaagtg                                                                                   >  1:608051/1‑140 (MQ=255)                                                 tatTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAaaagtgaaag                                                                               >  2:986103/1‑140 (MQ=255)                                                 tatTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAaaagtgaaag                                                                               >  1:92912/1‑140 (MQ=255)                                                  atTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAaaagtgaaagt                                                                              >  1:498642/1‑140 (MQ=255)                                                         tgatgaACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGc                                                                       >  1:268363/1‑140 (MQ=255)                                                                 cGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCcgcg                                                               >  1:894369/1‑140 (MQ=255)                                                                   aCCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGtt                                                             >  2:545082/1‑140 (MQ=255)                                                                   aCCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTAACAAGCTGGCCGCGtt                                                             >  1:418141/1‑140 (MQ=255)                                                                   aCCTTCTTCGACAAAGCGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGtt                                                             >  1:374946/1‑140 (MQ=255)                                                                                     aTGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCg                                           >  2:328001/1‑140 (MQ=255)                                                                                        gACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTa                                        <  1:651141/140‑1 (MQ=255)                                                                                             caTGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTa                                        <  1:718655/135‑1 (MQ=255)                                                                                             caTGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTa                                        >  2:718655/1‑135 (MQ=255)                                                                                             caTGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGt                                   >  1:641987/1‑140 (MQ=255)                                                                                                     gTTCTGTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTg                           >  2:314651/1‑140 (MQ=255)                                                                                                          gTCCGGGCAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTg                      >  2:744450/1‑140 (MQ=255)                                                                                                           tCCGGGCTATTGTGCCAGATGCGCTCCAGGCCATGATACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTgc                     >  2:255336/1‑140 (MQ=255)                                                                                                                 cAATGTTGCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAAc               <  2:641987/140‑1 (MQ=255)                                                                                                                        gCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGtt        <  2:348830/140‑1 (MQ=255)                                                                                                                        gCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGtt        <  2:109767/140‑1 (MQ=255)                                                                                                                        gCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGtt        <  1:744450/140‑1 (MQ=255)                                                                                                                        gCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGtt        <  1:545082/140‑1 (MQ=255)                                                                                                                        gCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGtt        <  2:92912/140‑1 (MQ=255)                                                                                                                        gCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGtt        <  1:314651/140‑1 (MQ=255)                                                                                                                        gCCAGATGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTAACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGtt        <  2:418141/140‑1 (MQ=255)                                                                                                                              tGCGCTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCt  >  2:564976/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                        GTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGTTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCT  >  NC_000913/1850433‑1850697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑