Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,913,124	T→C	T565A (ACG→GCG)	prc ←	tail‑specific protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,913,124	0	T	C	100.0%	191.8 / NA	54	T565A (ACG→GCG)	prc	tail‑specific protease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/25);  total (29/25)

CGTAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACG  >  NC_000913/1912989‑1913256                                                                                                                                        |                                                                                                                                     cGTAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTaaa                                                                                                                                  >  1:285274/1‑140 (MQ=255) cGTAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTaaa                                                                                                                                  >  1:614550/1‑140 (MQ=255)      ttCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTcc                                                                                                                             >  2:612963/1‑140 (MQ=255)          gagaAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGAt                                                                                                                         <  2:599911/140‑1 (MQ=255)           agaAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGAt                                                                                                                         <  2:786712/139‑1 (MQ=255)           agaAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGAt                                                                                                                         >  1:786712/1‑139 (MQ=255)                cGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTcaca                                                                                                                   >  1:219477/1‑140 (MQ=255)                        cgcTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTcgc                                                                                                           <  2:285274/140‑1 (MQ=255)                         gcTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTcgcg                                                                                                          <  2:917013/140‑1 (MQ=255)                            tGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCa                                                                                                       >  1:727352/1‑140 (MQ=255)                             gTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTcgc                                                                                                           <  2:678723/135‑1 (MQ=255)                             gTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTcgc                                                                                                           >  1:678723/1‑135 (MQ=255)                             gTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCAt                                                                                                      >  1:939756/1‑140 (MQ=255)                             gTCCGTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCAt                                                                                                      <  1:545865/140‑1 (MQ=255)                                cTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCaa                                                                                                   <  2:614550/140‑1 (MQ=255)                                   cATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTcgc                                                                                                           <  1:659653/129‑1 (MQ=255)                                   cATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTcgc                                                                                                           >  2:659653/1‑129 (MQ=255)                                   cATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGc                                                                                                <  1:612963/140‑1 (MQ=255)                                                    cgATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGcgcg                                                                               <  2:939756/140‑1 (MQ=255)                                                               atgatgTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTACTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGaa                                                                    >  1:560022/1‑140 (MQ=255)                                                                 gatgTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAAtt                                                                  >  2:618570/1‑140 (MQ=255)                                                                   tgTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGaa                                                                    <  2:844255/136‑1 (MQ=255)                                                                   tgTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGaa                                                                    >  1:844255/1‑136 (MQ=255)                                                                   tgTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTtc                                                                >  1:772145/1‑140 (MQ=255)                                                                   tgTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTtc                                                                >  2:343394/1‑140 (MQ=255)                                                                         ggAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCg                                                                      <  2:889783/128‑1 (MQ=255)                                                                         ggAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCg                                                                      >  1:889783/1‑128 (MQ=255)                                                                         ggAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCAccc                                                          >  2:435535/1‑140 (MQ=255)                                                                            aCTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACccgtt                                                       <  2:269847/140‑1 (MQ=255)                                                                               cAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACccgtttcc                                                    >  1:750974/1‑140 (MQ=255)                                                                                aGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACccgtttccg                                                   >  1:627033/1‑140 (MQ=255)                                                                                      tttCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTttctt                                             <  1:343394/140‑1 (MQ=255)                                                                                       ttCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTttcttc                                            >  1:997667/1‑140 (MQ=255)                                                                                        tCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCa                                           >  2:855403/1‑140 (MQ=255)                                                                                            gATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTAc                                       >  1:197547/1‑140 (MQ=255)                                                                                                cgcgCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGt                                   <  2:97014/140‑1 (MQ=255)                                                                                                cgcgCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGt                                   <  2:197547/140‑1 (MQ=255)                                                                                                  cgcATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCg                                 <  2:627033/140‑1 (MQ=255)                                                                                                          gTTCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCatgatg                         >  1:193537/1‑140 (MQ=255)                                                                                                            tCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCatg                            >  1:497334/1‑135 (MQ=255)                                                                                                            tCCTTCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCatg                            <  2:497334/135‑1 (MQ=255)                                                                                                               ttCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCGCCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTc                    <  2:219477/140‑1 (MQ=255)                                                                                                               ttCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTc                    >  1:887976/1‑140 (MQ=255)                                                                                                               ttCAGCAGCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTc                    >  1:517683/1‑140 (MQ=255)                                                                                                                 cagcagCTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAATCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTg                  >  1:977421/1‑140 (MQ=255)                                                                                                                   gcagcTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGc                >  1:988514/1‑140 (MQ=255)                                                                                                                    cagcTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCa                              <  2:695065/125‑1 (MQ=255)                                                                                                                    cagcTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCa                              >  1:695065/1‑125 (MQ=255)                                                                                                                       cTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTa            <  1:618570/140‑1 (MQ=255)                                                                                                                       cTCCGGTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTa            <  2:560022/140‑1 (MQ=255)                                                                                                                            gTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGtt             >  2:813558/1‑134 (MQ=255)                                                                                                                            gTTCAAAGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGtt             <  1:813558/134‑1 (MQ=255)                                                                                                                                 aaGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACg  <  2:193537/140‑1 (MQ=255)                                                                                                                                 aaGGCCGCTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACg  <  1:435535/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     CGTAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACG  >  NC_000913/1912989‑1913256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑