Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,022,646	A→G	T11A (ACT→GCT)	fliQ →	flagellar biosynthesis protein FliQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,022,646	0	A	G	100.0%	146.0 / NA	42	T11A (ACT→GCT)	fliQ	flagellar biosynthesis protein FliQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/15);  total (27/15)

TTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCA  >  NC_000913/2022518‑2022781                                                                                                                                 |                                                                                                                                        ttGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATg                                                                                                                              >  1:256110/1‑140 (MQ=255)     tctGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAg                                                                                                                          >  1:357408/1‑140 (MQ=255)     tctGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAg                                                                                                                          >  2:371323/1‑140 (MQ=255)         cccTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTcgc                                                                                                                      >  1:249256/1‑140 (MQ=255)          ccTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTcgcg                                                                                                                     <  2:357408/140‑1 (MQ=255)          ccTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTcgcg                                                                                                                     <  2:256110/140‑1 (MQ=255)             ttAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTg                                                                                                                  >  2:564572/1‑140 (MQ=255)              tAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTgg                                                                                                                 >  1:149902/1‑140 (MQ=255)                   tGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCa                                                                                                               <  2:670167/137‑1 (MQ=255)                   tGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCa                                                                                                               >  1:670167/1‑137 (MQ=255)                      tGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCt                                                                                                         >  1:713048/1‑140 (MQ=255)                       gCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTg                                                                                                        <  2:140649/140‑1 (MQ=255)                          gTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGccc                                                                                                     <  2:604403/140‑1 (MQ=255)                               tACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTa                                                                                                <  2:249256/140‑1 (MQ=255)                                     tggATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTAttgttg                                                                                          >  2:598068/1‑140 (MQ=255)                                         tggctggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAg                                                                                      >  2:455723/1‑140 (MQ=255)                                           gctggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCg                                                                                    <  1:674769/140‑1 (MQ=255)                                           gctggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCg                                                                                    <  1:455723/140‑1 (MQ=255)                                              ggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTg                                                                                 >  1:622276/1‑140 (MQ=255)                                              ggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTg                                                                                 >  1:630259/1‑140 (MQ=255)                                              ggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTg                                                                                 >  1:889572/1‑140 (MQ=255)                                                cAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGt                                                                               <  2:630259/140‑1 (MQ=255)                                                         gTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCt                                                                      >  2:762209/1‑140 (MQ=255)                                                               tCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTatcat                                                                <  2:713048/140‑1 (MQ=255)                                                               tCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTatcat                                                                <  2:622276/140‑1 (MQ=255)                                                                      cgcAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACgg                                                                          <  2:798877/123‑1 (MQ=255)                                                                      cgcAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACgg                                                                          >  1:798877/1‑123 (MQ=255)                                                                        cAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTAtttt                                                       >  1:492549/1‑140 (MQ=255)                                                                           agCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCa                                                    <  1:564572/140‑1 (MQ=255)                                                                             cTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAgg                                                  <  2:492549/140‑1 (MQ=255)                                                                                     gCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACg                                          <  1:762209/140‑1 (MQ=255)                                                                                       tAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCa                                        >  1:589374/1‑140 (MQ=255)                                                                                                aaaaTGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGa                               >  2:831414/1‑140 (MQ=255)                                                                                                 aaaTGACACCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGaa                              >  2:445158/1‑140 (MQ=255)                                                                                                      acacCTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGAc                         >  1:536977/1‑140 (MQ=255)                                                                                                         ccTGAATCGGTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCt                      >  2:235357/1‑140 (MQ=255)                                                                                                                  gTCATGATGATGGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTAt             >  1:964639/1‑140 (MQ=255)                                                                                                                       gatgatgGGGGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGa        >  2:940322/1‑140 (MQ=255)                                                                                                                             ggggGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAatca  >  1:553704/1‑140 (MQ=255)                                                                                                                             ggggGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAatca  >  1:571972/1‑140 (MQ=255)                                                                                                                             ggggGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAatca  >  1:883426/1‑140 (MQ=255)                                                                                                                             ggggGCTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAatca  >  2:95918/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                        TTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCA  >  NC_000913/2022518‑2022781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑