Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,078,657	A→G	Y28C (TAC→TGC)	yoeF →	CP4‑44 prophage; putative uncharacterized protein YoeF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,078,657	0	A	G	100.0%	210.0 / NA	59	Y28C (TAC→TGC)	yoeF	CP4‑44 prophage; putative uncharacterized protein YoeF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/27);  total (32/27)

GCGTCAACGGAGCACCGTATTGACGCTTATTTATTGGTGAGAACTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATACCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGTCATTGGAGTAGTAACCAAAGCAGA  >  NC_000913/2078523‑2078786                                                                                                                                       |                                                                                                                                  gCGTCAACGGAGCACCGTATTGACGCTTATTTATTGGTGAGAACTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGtt                                                                                                                              <  2:726693/140‑1 (MQ=255)      aaCGGAGCACCGTATTGACGCTTATTTATTGGTGAGAACTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGtt                                                                                                                              <  2:622360/135‑1 (MQ=255)      aaCGGAGCACCGTATTGACGCTTATTTATTGGTGAGAACTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGtt                                                                                                                              >  1:622360/1‑135 (MQ=255)      aaCGGAGCACCGTATTGACGCTTATTTATTGGTGAGAACTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGAc                                                                                                                         >  2:953897/1‑140 (MQ=255)      aaCGGAGCACCGTATTGACGCTTATTTATTGGTGAGAACTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGAc                                                                                                                         >  2:782919/1‑140 (MQ=255)          gAGCACCGTATTGACGCTTATTTATTGGTGAGAACTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCa                                                                                                                     <  1:669135/140‑1 (MQ=255)           aGCACCGTATTGACGCTTATTTATTGGTGAGAACTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGc                                                                                                                      >  2:837568/1‑138 (MQ=255)           aGCACCGTATTGACGCTTATTTATTGGTGAGAACTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGc                                                                                                                      <  1:837568/138‑1 (MQ=255)                     tGACGCTTATTTATTGGTGAGAACTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCtt                                                                                                          >  1:797280/1‑140 (MQ=255)                     tGACGCTTATTTATTGGTGAGAACTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCtt                                                                                                          >  1:643066/1‑140 (MQ=255)                          cTTATTTATTGGTGAGAACTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTg                                                                                                     >  2:137197/1‑140 (MQ=255)                                       gagaACTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGa                                                                                        >  1:961668/1‑140 (MQ=255)                                        agaACTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGaa                                                                                       >  2:609022/1‑140 (MQ=255)                                        agaACTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGaa                                                                                       >  2:854362/1‑140 (MQ=255)                                        agaACTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGaa                                                                                       >  2:593164/1‑140 (MQ=255)                                               cGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTAAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAgc                                                                                >  1:600482/1‑140 (MQ=255)                                                   ccATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTAAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTg                                                                            <  2:600482/140‑1 (MQ=255)                                                    cATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGa                                                                           >  2:198222/1‑140 (MQ=255)                                                     aTGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGaa                                                                          <  1:782919/140‑1 (MQ=255)                                                       ggCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGc                                                                        >  1:400905/1‑140 (MQ=255)                                                            gAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGACTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGaa                                                                   >  2:459735/1‑140 (MQ=255)                                                                      acacGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTaa                                                         >  1:484750/1‑140 (MQ=255)                                                                       cacGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTaaa                                                        >  1:423459/1‑140 (MQ=255)                                                                        acGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTaaaa                                                       <  2:423459/140‑1 (MQ=255)                                                                         cGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGc                                                                        <  2:10357/122‑1 (MQ=255)                                                                         cGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGc                                                                        >  1:10357/1‑122 (MQ=255)                                                                             tGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGcc                                                  <  2:139812/140‑1 (MQ=255)                                                                                 gggCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTAc                                              >  1:352813/1‑140 (MQ=255)                                                                                 gggCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTAc                                              >  2:475541/1‑140 (MQ=255)                                                                                  ggCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTAcc                                             <  1:137197/140‑1 (MQ=255)                                                                                  ggCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTAcc                                             <  2:643066/140‑1 (MQ=255)                                                                                     cATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCggg                                          >  1:693717/1‑140 (MQ=255)                                                                                      aTTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGc                                         <  1:475541/140‑1 (MQ=255)                                                                                            gCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGaa                                   >  1:373774/1‑140 (MQ=255)                                                                                             cAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAAt                                  >  1:338244/1‑140 (MQ=255)                                                                                                tATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCAc                               <  2:664178/140‑1 (MQ=255)                                                                                                 aTGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCAcc                              <  1:953897/140‑1 (MQ=255)                                                                                                  tGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATTTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTAc                                              <  2:270540/123‑1 (MQ=255)                                                                                                  tGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTAc                                              <  2:810867/123‑1 (MQ=255)                                                                                                  tGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTAc                                              >  1:810867/1‑123 (MQ=255)                                                                                                  tGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTAc                                              >  1:270540/1‑123 (MQ=255)                                                                                                  tGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCAccc                             >  1:869482/1‑140 (MQ=255)                                                                                                   gCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCg                            <  2:484750/140‑1 (MQ=255)                                                                                                   gCTCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCg                            <  2:577220/140‑1 (MQ=255)                                                                                                     tCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGt                          <  1:854362/140‑1 (MQ=255)                                                                                                     tCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGt                          <  1:593164/140‑1 (MQ=255)                                                                                                     tCAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGt                          <  2:693717/140‑1 (MQ=255)                                                                                                      cAGAATATGGCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATT‑‑AGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGTCAt                       <  2:400905/140‑1 (MQ=255)                                                                                                              ggCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCg                            <  2:502090/129‑1 (MQ=255)                                                                                                              ggCGCAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCg                            >  1:502090/1‑129 (MQ=255)                                                                                                                cgcAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCa                                >  1:206480/1‑123 (MQ=255)                                                                                                                cgcAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCa                                <  2:206480/123‑1 (MQ=255)                                                                                                                cgcAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGt                          >  2:116607/1‑129 (MQ=255)                                                                                                                cgcAGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGt                          <  1:116607/129‑1 (MQ=255)                                                                                                                   aGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGTCa                        <  2:661745/128‑1 (MQ=255)                                                                                                                   aGATACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGTCa                        >  1:661745/1‑128 (MQ=255)                                                                                                                      tACGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGTCATTGGAGTAGTAACCa         <  2:338244/140‑1 (MQ=255)                                                                                                                       aCGCTTCCGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGTCATTGGAGTAGTAACCaa        >  1:335497/1‑140 (MQ=255)                                                                                                                             ccGGATCGATGCCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGTCATTGGAGTAGTAACCAAAGCAGa  >  1:896835/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                  GCGTCAACGGAGCACCGTATTGACGCTTATTTATTGGTGAGAACTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAGGGCCATTCCGGCAGTATGCTCAGAATATGGCGCAGATACGCTTCCGGATCGATACCGTTCAGACGGCAGGTGCCTGCTTTTCTGGCAACAAGCAGGAATCTCAGCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGTCATTGGAGTAGTAACCAAAGCAGA  >  NC_000913/2078523‑2078786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑