Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,264,292	T→C	F144L (TTC→CTC)	setB →	sugar exporter SetB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,264,292	0	T	C	100.0%	188.8 / NA	54	F144L (TTC→CTC)	setB	sugar exporter SetB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/29);  total (25/29)

TTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCG  >  NC_000913/2264155‑2264421                                                                                                                                          |                                                                                                                                  tttttGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTc                                                                                                                                 >  2:610195/1‑140 (MQ=255)           gAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCttttt                                                                                                                       >  1:819717/1‑140 (MQ=255)           gAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCttttt                                                                                                                       >  1:567454/1‑140 (MQ=255)               cGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTAcg                                                                                                                   <  2:79666/140‑1 (MQ=255)                gCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTAcgc                                                                                                                  <  2:285071/140‑1 (MQ=255)                gCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTAcgc                                                                                                                  <  2:387002/140‑1 (MQ=255)                             tttGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCAc                                                                                                     <  2:817436/140‑1 (MQ=255)                              ttGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACt                                                                                                    >  1:792474/1‑140 (MQ=255)                                 cTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGc                                                                                                 >  1:244560/1‑140 (MQ=255)                                          ggCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCAt                                                                                        <  2:567454/140‑1 (MQ=255)                                              tctttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGc                                                                                    >  2:868444/1‑140 (MQ=255)                                               ctttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGcc                                                                                   <  1:897157/140‑1 (MQ=255)                                                tttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGccc                                                                                  >  1:550994/1‑140 (MQ=255)                                                  tcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCAc                                                                                >  2:519007/1‑140 (MQ=255)                                                    ttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCg                                                                              <  2:501989/140‑1 (MQ=255)                                                    ttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCg                                                                              <  1:364515/140‑1 (MQ=255)                                                     tAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGc                                                                             <  2:8383/140‑1 (MQ=255)                                                     tAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGc                                                                             <  2:337077/140‑1 (MQ=255)                                                        cagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTgg                                                                          >  1:632005/1‑140 (MQ=255)                                                         agcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGc                                                                         <  2:632005/140‑1 (MQ=255)                                                         agcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGc                                                                         >  2:82066/1‑140 (MQ=255)                                                              tGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATg                                                                    >  2:58089/1‑140 (MQ=255)                                                                gCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGcc                                                                  <  2:132099/140‑1 (MQ=255)                                                                gCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGcc                                                                  <  2:931269/140‑1 (MQ=255)                                                                       cGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGa                                                           <  2:966979/140‑1 (MQ=255)                                                                       cGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGa                                                           <  1:565725/140‑1 (MQ=255)                                                                         cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATg                                                         >  1:71692/1‑140 (MQ=255)                                                                         cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATg                                                         >  2:295253/1‑140 (MQ=255)                                                                                gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCa                                                  >  1:810206/1‑140 (MQ=255)                                                                                gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCa                                                  >  1:171530/1‑140 (MQ=255)                                                                                       tttGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTAc                                           <  2:819717/140‑1 (MQ=255)                                                                                       tttGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTAc                                           <  2:810206/140‑1 (MQ=255)                                                                                       tttGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTAc                                           <  1:45228/140‑1 (MQ=255)                                                                                       tttGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTAc                                           <  2:550994/140‑1 (MQ=255)                                                                                       tttGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTAc                                           <  2:171530/140‑1 (MQ=255)                                                                                         tgcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACgg                                         >  1:676393/1‑140 (MQ=255)                                                                                         tgcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACgg                                         <  1:519007/140‑1 (MQ=255)                                                                                           cccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTa                                       <  1:317988/140‑1 (MQ=255)                                                                                                gcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTa                                  >  2:50722/1‑140 (MQ=255)                                                                                                gcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTa                                  >  2:326296/1‑140 (MQ=255)                                                                                                 cccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTAt                                 >  2:542190/1‑140 (MQ=255)                                                                                                 cccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTAt                                 <  2:676393/140‑1 (MQ=255)                                                                                                          aTGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCa                        >  2:987971/1‑140 (MQ=255)                                                                                                           tGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAg                       >  2:352967/1‑140 (MQ=255)                                                                                                           tGCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACCCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCCTAGCGGTGGGTTTCAGCTTTACGGTAATCTATCTGAGCGCac                       >  2:477934/1‑139 (MQ=255)                                                                                                            gCCGACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAGc                      >  1:716441/1‑140 (MQ=255)                                                                                                               gACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAGCGGt                   <  2:71692/140‑1 (MQ=255)                                                                                                               gACAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAGCGGt                   <  2:716441/140‑1 (MQ=255)                                                                                                                aCAAAACCGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAGCGGTa                  >  1:938774/1‑140 (MQ=255)                                                                                                                      ccGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAGCGGTAGCGttt            <  1:326296/140‑1 (MQ=255)                                                                                                                       cGGACGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAGCGGTAGCGTTTa           >  2:108473/1‑140 (MQ=255)                                                                                                                           cGTGAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAGCGGTAGCGTTTATTGt       <  2:938774/140‑1 (MQ=255)                                                                                                                              gAGGCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTg    <  1:542190/140‑1 (MQ=255)                                                                                                                                ggCGGTGATGCTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCg  <  2:336373/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  TTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCG  >  NC_000913/2264155‑2264421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑