Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,305,722	C→T	A345T (GCC→ACC)	mqo ←	malate:quinone oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,305,722	0	C	T	100.0%	155.4 / NA	45	A345T (GCC→ACC)	mqo	malate:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/21);  total (24/21)

CACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGG  >  NC_000913/2305585‑2305852                                                                                                                                          |                                                                                                                                   cACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTaa                                                                                                                                  >  2:296806/1‑140 (MQ=255)   ccTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAAt                                                                                                                                >  1:866046/1‑140 (MQ=255)        cTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGccc                                                                                                                           >  1:273999/1‑140 (MQ=255)           aCCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCaaa                                                                                                                        >  1:447675/1‑140 (MQ=255)               gATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGc                                                                                                                    >  2:49859/1‑140 (MQ=255)                      cACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTAcgc                                                                                                             <  2:447675/140‑1 (MQ=255)                      cACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTAcgc                                                                                                             <  2:273999/140‑1 (MQ=255)                          aGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGttt                                                                                                         >  2:200374/1‑140 (MQ=255)                                aaaTTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTc                                                                                                   <  2:543034/140‑1 (MQ=255)                                               aCGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAAC‑CGGGTATCg                                                                                   <  2:632385/140‑1 (MQ=255)                                                cGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCg                                                                                   >  2:393498/1‑140 (MQ=255)                                                 gTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGa                                                                                  <  2:107659/140‑1 (MQ=255)                                                         tcGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCg                                                                                   >  2:270323/1‑131 (MQ=255)                                                         tcGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCg                                                                                   <  1:270323/131‑1 (MQ=255)                                                          cGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGaa                                                                         <  1:42276/140‑1 (MQ=255)                                                               tCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGAc                                                                    >  2:358868/1‑140 (MQ=255)                                                                  cGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATc                                                                 >  1:105776/1‑140 (MQ=255)                                                                         ggtggtggtGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGgtggtg                                                          <  1:200374/140‑1 (MQ=255)                                                                            ggtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCggtgg                                                            <  2:47819/135‑1 (MQ=255)                                                                            ggtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCggtgg                                                            >  1:47819/1‑135 (MQ=255)                                                                             gtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGcc                                                      >  1:361404/1‑140 (MQ=255)                                                                             gtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGcc                                                      <  2:105776/140‑1 (MQ=255)                                                                               ggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCaa                                                    >  2:471650/1‑140 (MQ=255)                                                                                   gAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGa                                                <  2:361404/140‑1 (MQ=255)                                                                                        cATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCtt                                           <  1:49859/140‑1 (MQ=255)                                                                                        cATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCtt                                           <  1:471650/140‑1 (MQ=255)                                                                                           tAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTAc                                        >  1:215728/1‑140 (MQ=255)                                                                                                   acaATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTGTCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCtt                                           >  1:367743/1‑129 (MQ=255)                                                                                                   acaATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTGTCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCtt                                           <  2:367743/129‑1 (MQ=255)                                                                                                   acaATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACgg                                                 >  2:909051/1‑123 (MQ=255)                                                                                                   acaATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACgg                                                 <  1:909051/123‑1 (MQ=255)                                                                                                     aaTGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCtt                              >  2:71021/1‑140 (MQ=255)                                                                                                       tGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCg                            >  1:86175/1‑140 (MQ=255)                                                                                                       tGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCg                            >  1:202472/1‑140 (MQ=255)                                                                                                          aCCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGc                           <  2:360569/138‑1 (MQ=255)                                                                                                          aCCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGc                           >  1:360569/1‑138 (MQ=255)                                                                                                          aCCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCa                         >  2:332218/1‑140 (MQ=255)                                                                                                          aCCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCa                         >  2:133374/1‑140 (MQ=255)                                                                                                            cGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                       <  1:741742/140‑1 (MQ=255)                                                                                                            cGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                       <  1:358868/140‑1 (MQ=255)                                                                                                            cGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGCAAACCTTCGCCAGa                       <  2:215728/140‑1 (MQ=255)                                                                                                               ttttGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATgg                    <  1:133374/140‑1 (MQ=255)                                                                                                                tttGAGGAATTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGt                   <  1:949929/140‑1 (MQ=255)                                                                                                                        aTTTGGTTGAGAAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCAAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAAcc           >  2:436091/1‑140 (MQ=255)                                                                                                                                 agaAGGTGGTAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCggg  >  2:941090/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                   CACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGG  >  NC_000913/2305585‑2305852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑