Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,360,919	A→G	F166L (TTC→CTC)	rhmD ←	L‑rhamnonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,360,919	0	A	G	100.0%	181.4 / NA	52	F166L (TTC→CTC)	rhmD	L‑rhamnonate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/31);  total (21/31)

CATAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAACTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAG  >  NC_000913/2360800‑2361049                                                                                                                          |                                                                                                                                   cATAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAg                                                                                                                <  2:630705/140‑1 (MQ=255)  aTAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAgc                                                                                                               >  1:439190/1‑140 (MQ=255)   tAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAgcg                                                                                                              <  2:439190/140‑1 (MQ=255)          aTCTTTGCGGATCCCCGCATCGCCATCATGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTa                                                                                                       <  1:849667/140‑1 (MQ=255)               tGCGGATCCCCGCATCGCCATCATGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAgcg                                                                                                              <  1:455325/128‑1 (MQ=255)               tGCGGATCCCCGCATCGCCATCATGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAgcg                                                                                                              >  2:455325/1‑128 (MQ=255)                       cccGCATCGCCATCATGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAAc                                                                                          >  1:933631/1‑140 (MQ=255)                       cccGCATCGCCATCATGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAAc                                                                                          >  2:5636/1‑140 (MQ=255)                        ccGCATCGCCATCATGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAAcc                                                                                         <  1:141092/140‑1 (MQ=255)                              cGCCATCATGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGTGGGGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGaa                                                                                     >  1:817795/1‑140 (MQ=255)                              cGCCATCATGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGc                                                                                   >  2:721867/1‑140 (MQ=255)                                   tcatGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTtata                                                                                             >  1:323613/1‑125 (MQ=255)                                   tcatGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTtata                                                                                             <  2:323613/125‑1 (MQ=255)                                     atGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCa                                                                            <  2:997379/140‑1 (MQ=255)                                     atGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCa                                                                            <  1:565219/140‑1 (MQ=255)                                     atGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCa                                                                            <  1:805162/140‑1 (MQ=255)                                        tgGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGccc                                                                                 >  1:388563/1‑132 (MQ=255)                                        tgGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGccc                                                                                 <  2:388563/132‑1 (MQ=255)                                          gCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTg                                                                       >  2:546301/1‑140 (MQ=255)                                             cccAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCg                                                                    >  2:199125/1‑140 (MQ=255)                                              ccAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGa                                                                   <  1:152065/140‑1 (MQ=255)                                              ccAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGa                                                                   <  1:721867/140‑1 (MQ=255)                                               cAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGaa                                                                  >  1:943841/1‑140 (MQ=255)                                               cAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGaa                                                                  >  2:43494/1‑140 (MQ=255)                                                aGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAAc                                                                 <  2:943841/140‑1 (MQ=255)                                                     gTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATc                                                            >  2:246586/1‑140 (MQ=255)                                                        ggCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCca                                                         <  1:199125/140‑1 (MQ=255)                                                        ggCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCca                                                         <  2:992926/140‑1 (MQ=255)                                                        ggCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCca                                                         <  2:105627/140‑1 (MQ=255)                                                            ttttGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACaga                                                     <  1:419319/140‑1 (MQ=255)                                                              ttGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGc                                                   <  1:977642/140‑1 (MQ=255)                                                                   aCCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGAt                                              <  2:843631/140‑1 (MQ=255)                                                                            gCCCATCTCTTTTGCCAGATCCGGACGCGCGCCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGACCAGATCCCACAGAGCCAGATCGACACAAg                                     <  2:702219/140‑1 (MQ=255)                                                                            gCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAg                                     <  1:175685/140‑1 (MQ=255)                                                                               cATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGaaa                                  >  2:368882/1‑140 (MQ=255)                                                                               cATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGaaa                                  >  1:746280/1‑140 (MQ=255)                                                                                  ctctTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCg                               <  1:43494/140‑1 (MQ=255)                                                                                           cAGATCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATtcatca                      >  2:730032/1‑140 (MQ=255)                                                                                               tCCGGACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAg                  >  2:343514/1‑140 (MQ=255)                                                                                                 cGGACGCGCACCTGTGGGGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAg                  <  2:817795/140‑1 (MQ=255)                                                                                                   gACGCGCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGcca              >  2:218942/1‑140 (MQ=255)                                                                                                     cgcgCACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGccacc            <  1:546301/140‑1 (MQ=255)                                                                                                        gcACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAGCAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCAc                     >  2:732330/1‑128 (MQ=255)                                                                                                        gcACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAGCAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCAc                     <  1:732330/128‑1 (MQ=255)                                                                                                         cACCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCagag        >  2:168681/1‑140 (MQ=255)                                                                                                          aCCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGc       <  1:218942/140‑1 (MQ=255)                                                                                                          aCCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGc       <  2:746280/140‑1 (MQ=255)                                                                                                          aCCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGc       <  1:368882/140‑1 (MQ=255)                                                                                                          aCCTGT‑‑GGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGc       <  1:5636/140‑1 (MQ=255)                                                                                                              gt‑‑gGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGa   <  1:198198/140‑1 (MQ=255)                                                                                                              gt‑‑gGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGa   <  2:545787/140‑1 (MQ=255)                                                                                                              gt‑‑gGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGa   >  1:545787/1‑140 (MQ=255)                                                                                                               t‑‑gGCGTAGAGCTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAg  <  1:168681/140‑1 (MQ=255)                                                                                                                          |                                                                                                                                   CATAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGT‑‑GGCGTAGAACTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAG  >  NC_000913/2360800‑2361049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑