Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,364,181	T→C	Y32C (TAC→TGC)	yfaZ ←	putative porin YfaZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,364,181	0	T	C	100.0%	132.2 / NA	40	Y32C (TAC→TGC)	yfaZ	putative porin YfaZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/17);  total (23/17)

CGCCATTAACGGCCCGAGAGGCAGATTCAACCCCAGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAG  >  NC_000913/2364045‑2364315                                                                                                                                         |                                                                                                                                       cGCCATTAACGGCCCGAGAGGCAGATTCAACCCCAGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCAtt                                                                                                                                     >  2:410292/1‑140 (MQ=255)        aaCGGCCCGAGAGGCAGATTCAACCCCAGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTAcc                                                                                                                              >  1:2975/1‑140 (MQ=255)           ggCCCGAGAGGCAGATTCAACCCCAGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGc                                                                                                                           <  1:217087/140‑1 (MQ=255)             cccGAGAGGCAGATTCAACCCCAGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCTCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCt                                                                                                                         >  2:942366/1‑140 (MQ=255)               cGAGAGGCAGATTCAACCCCAGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGCGCATTCTTTACCCGCCTGa                                                                                                                       >  2:66379/1‑140 (MQ=255)                       aGATTCAACCCCAGCCCCACGCCAGCGACATCACCGTCGCCGTCTTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATGGGTGCATTCTTTACCCGCCTGACCGCTGAt                                                                                                               <  2:160379/140‑1 (MQ=255)                         aTTCAACCCCAGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGc                                                                                                             <  1:943555/140‑1 (MQ=255)                         aTTCAACCCCAGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGc                                                                                                             <  1:542767/140‑1 (MQ=255)                            cAACCCCAGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTca                                                                                                          <  1:21792/140‑1 (MQ=255)                            cAACCCCAGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTca                                                                                                          <  1:760030/140‑1 (MQ=255)                                   aGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTcattgcatt                                                                                                   >  2:342059/1‑140 (MQ=255)                                   aGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTcattgcatt                                                                                                   >  2:235382/1‑140 (MQ=255)                                               gCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGa                                                                                       >  2:284383/1‑140 (MQ=255)                                                cGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGa                                                                                             >  1:674033/1‑133 (MQ=255)                                                cGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGa                                                                                             <  2:674033/133‑1 (MQ=255)                                                cGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGaa                                                                                      >  1:830688/1‑140 (MQ=255)                                                       aCCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGCGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACa                                                                               <  1:66379/140‑1 (MQ=255)                                                            cgtcgtcgtTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCAtt                                                                          <  1:342059/140‑1 (MQ=255)                                                                gtcgtTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCgg                                                                      >  1:331415/1‑140 (MQ=255)                                                                           tCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACATCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCt                                                           >  1:838904/1‑140 (MQ=255)                                                                           tCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCt                                                           >  1:738120/1‑140 (MQ=255)                                                                              aGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAgc                                                        >  2:886300/1‑140 (MQ=255)                                                                                  aCCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCaa                                                    >  1:569799/1‑140 (MQ=255)                                                                                   ccGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGGCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAAt                                                   >  1:907914/1‑140 (MQ=255)                                                                                   ccGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAAt                                                   >  2:624421/1‑140 (MQ=255)                                                                                     gCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAAttt                                                 >  1:599926/1‑140 (MQ=255)                                                                                        tAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAAtttttt                                              <  1:624421/140‑1 (MQ=255)                                                                                          aCGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTc                                            >  1:296864/1‑140 (MQ=255)                                                                                           cGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCa                                           <  2:296864/140‑1 (MQ=255)                                                                                                ggCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATc                                      <  1:886300/140‑1 (MQ=255)                                                                                                 gCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATcc                                     >  2:993660/1‑140 (MQ=255)                                                                                                      tcgtTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCttt                                >  2:357986/1‑140 (MQ=255)                                                                                                      tcgtTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCttt                                >  2:757775/1‑140 (MQ=255)                                                                                                        gtTTCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCCGCTAGCGCAATTTTTTTCATTATCCCCTTTcc                              <  2:830688/140‑1 (MQ=255)                                                                                                          ttCAGTCCCAAAACCGACACCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCca                            <  1:775482/140‑1 (MQ=255)                                                                                                                          acacCAATATTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCaaa            <  2:331415/140‑1 (MQ=255)                                                                                                                                 tatTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTcc       <  2:258991/138‑1 (MQ=255)                                                                                                                                 tatTGGTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTcc       >  1:258991/1‑138 (MQ=255)                                                                                                                                     ggTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAg  >  1:224683/1‑139 (MQ=255)                                                                                                                                     ggTGCATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAg  <  2:224683/139‑1 (MQ=255)                                                                                                                                      gtgcATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCaaa            <  1:974651/128‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CGCCATTAACGGCCCGAGAGGCAGATTCAACCCCAGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAG  >  NC_000913/2364045‑2364315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑