Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,408,980	T→C	V40A (GTC→GCC)	yfbR →	dCMP phosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,408,980	0	T	C	100.0%	242.6 / NA	69	V40A (GTC→GCC)	yfbR	dCMP phosphohydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/34);  total (35/34)

CATACGTAAGGTCACTATGAAACAGAGCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGTCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGG  >  NC_000913/2408846‑2409105                                                                                                                                       |                                                                                                                              catACGTAAGGTCACTATGAAACAGAGCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGccc                                                                                                                          >  1:164772/1‑140 (MQ=255)       tAAGGTCACTATGAAACAGAGCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATgcgc                                                                                                                    >  2:331542/1‑140 (MQ=255)       tAAGGTCACTATGAAACAGAGCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATgcgc                                                                                                                    >  1:944826/1‑140 (MQ=255)       tAAGGTCACTATGAAACAGAGCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATgcgc                                                                                                                    >  1:826499/1‑140 (MQ=255)         aGGTCACTATGAAACAGAGCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTg                                                                                                                  >  1:187259/1‑140 (MQ=255)         aGGTCACTATGAAACAGAGCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTg                                                                                                                  >  2:815979/1‑140 (MQ=255)              aCTATGAAACAGAGCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGc                                                                                                             >  2:950870/1‑140 (MQ=255)               cTATGAAACAGAGCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCt                                                                                                            >  2:556167/1‑140 (MQ=255)                tATGAAACAGAGCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTa                                                                                                           >  1:224131/1‑140 (MQ=255)                      aCAGAGCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCaaaa                                                                                                     >  2:70099/1‑140 (MQ=255)                      aCAGAGCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCaaaa                                                                                                     >  1:207519/1‑140 (MQ=255)                        agagCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAAt                                                                                                   >  1:696648/1‑140 (MQ=255)                          agCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGc                                                                                                             >  2:829146/1‑128 (MQ=255)                          agCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGc                                                                                                             <  1:829146/128‑1 (MQ=255)                          agCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCg                                                                                                 >  1:662169/1‑140 (MQ=255)                          agCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCg                                                                                                 >  1:172028/1‑140 (MQ=255)                          agCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCg                                                                                                 >  2:430085/1‑140 (MQ=255)                          agCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCg                                                                                                 >  1:367466/1‑140 (MQ=255)                              aTTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGaaaa                                                                                             >  1:467986/1‑140 (MQ=255)                                ttttttGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAAtt                                                                                           <  2:826499/140‑1 (MQ=255)                                  ttttGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTg                                                                                         <  2:367466/140‑1 (MQ=255)                                   tttGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTgg                                                                                        <  2:944826/140‑1 (MQ=255)                                   tttGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTgg                                                                                        <  2:207519/140‑1 (MQ=255)                                     tGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCg                                                                                      <  1:430085/140‑1 (MQ=255)                                       cccATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGt                                                                                    <  2:187259/140‑1 (MQ=255)                                            ctctcCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGt                                                                               <  1:815979/140‑1 (MQ=255)                                                 ccGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACg                                                                          >  1:940013/1‑140 (MQ=255)                                                    ccTGAAACTCGTTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCg                                                                       >  2:192465/1‑140 (MQ=255)                                                      tGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCg                                                                                      <  2:911392/123‑1 (MQ=255)                                                      tGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCg                                                                                      >  1:911392/1‑123 (MQ=255)                                                       gAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAAc                                                                    >  1:381840/1‑140 (MQ=255)                                                        aaaCTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACg                                                                   <  1:70099/140‑1 (MQ=255)                                                        aaaCTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACg                                                                   >  2:29974/1‑140 (MQ=255)                                                        aaaCTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACg                                                                   <  2:940013/140‑1 (MQ=255)                                                               ttAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCt                                                            <  1:29974/140‑1 (MQ=255)                                                                tAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTAt                                                                >  2:610511/1‑135 (MQ=255)                                                                tAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTAt                                                                <  1:610511/135‑1 (MQ=255)                                                                  aCCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTa                                                         <  1:580344/140‑1 (MQ=255)                                                                      cTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTAc                                                        >  1:977289/1‑137 (MQ=255)                                                                      cTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTAc                                                        <  2:977289/137‑1 (MQ=255)                                                                      cTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTgg                                                     >  1:56563/1‑140 (MQ=255)                                                                      cTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTgg                                                     >  2:240017/1‑140 (MQ=255)                                                                      cTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTgg                                                     <  1:331542/140‑1 (MQ=255)                                                                      cTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTgg                                                     <  2:172028/140‑1 (MQ=255)                                                                        ggCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCg                                                   <  1:192465/140‑1 (MQ=255)                                                                        ggCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCg                                                   <  1:556167/140‑1 (MQ=255)                                                                        ggCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCg                                                   <  1:619694/140‑1 (MQ=255)                                                                                   cgcAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGa                                        <  2:381840/140‑1 (MQ=255)                                                                                   cgcAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGa                                        <  1:270843/140‑1 (MQ=255)                                                                                      aaCGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGc                                     <  2:36895/140‑1 (MQ=255)                                                                                       aCGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCAc                                          >  1:390120/1‑134 (MQ=255)                                                                                       aCGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCAc                                          <  2:390120/134‑1 (MQ=255)                                                                                        cGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCt                                   >  1:998175/1‑140 (MQ=255)                                                                                        cGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCt                                   >  2:877556/1‑140 (MQ=255)                                                                                        cGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCt                                   >  1:870875/1‑140 (MQ=255)                                                                                           gCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCTCAg                                <  2:998175/140‑1 (MQ=255)                                                                                           gCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCTCAg                                <  2:56563/140‑1 (MQ=255)                                                                                                  gaAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCt                         <  1:950870/140‑1 (MQ=255)                                                                                                    aaaTGTGTCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCa                       >  1:342097/1‑140 (MQ=255)                                                                                                         tgtCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGc                  <  1:877556/140‑1 (MQ=255)                                                                                                           tCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGa                <  1:279353/140‑1 (MQ=255)                                                                                                           tCCGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGa                <  1:240017/140‑1 (MQ=255)                                                                                                            ccGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGAt               <  2:224131/140‑1 (MQ=255)                                                                                                            ccGAACACAGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGAt               <  2:140759/140‑1 (MQ=255)                                                                                                                   caGTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTa        <  2:357680/140‑1 (MQ=255)                                                                                                                     gTTTGCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACt      >  2:954033/1‑140 (MQ=255)                                                                                                                         gCAGGTAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCgg  >  2:920208/1‑140 (MQ=255)                                                                                                                              tAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTc     >  2:892248/1‑132 (MQ=255)                                                                                                                              tAGCGATGGCCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTc     <  1:892248/132‑1 (MQ=255)                                                                                                                                       |                                                                                                                              CATACGTAAGGTCACTATGAAACAGAGCCATTTTTTTGCCCATCTCTCCCGCCTGAAACTCATTAACCGCTGGCCGCTCATGCGCAACGTGCGGACGGAAAATGTGTCCGAACACAGTTTGCAGGTAGCGATGGTCGCCCATGCGCTGGCAGCTATCAAAAATCGAAAATTTGGCGGTAATGTCAACGCCGAACGTATCGCTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGG  >  NC_000913/2408846‑2409105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑