Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,411,793	A→G	G99G (GGT→GGC)	hxpA ←	hexitol phosphatase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,411,793	0	A	G	100.0%	101.1 / NA	30	G99G (GGT→GGC)	hxpA	hexitol phosphatase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/19);  total (11/19)

ATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAA  >  NC_000913/2411658‑2411926                                                                                                                                        |                                                                                                                                      aTACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTg                                                                                                                                   >  1:700794/1‑140 (MQ=255)  tACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACCCCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGc                                                                                                                                  <  2:626429/140‑1 (MQ=255)   aCGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCt                                                                                                                                 <  1:17676/140‑1 (MQ=255)        tCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTAtt                                                                                                                            >  1:869987/1‑140 (MQ=255)         cAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTAACAAACACCTCTGGTGCGGGAAGCCCAGCTACTTTATGGCGCGCTCGCGCTACCGGCATGGCGCCAGAAGTCACAATGGCCCACGGAATGGCTGCTTTAttt                                                                                                                           >  2:882591/1‑139 (MQ=255)               cTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGa                                                                                                                     <  2:869987/140‑1 (MQ=255)               cTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGa                                                                                                                     <  2:417023/140‑1 (MQ=255)                    tttCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAg                                                                                                                <  2:700794/140‑1 (MQ=255)                     ttCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGt                                                                                                               <  2:724662/140‑1 (MQ=255)                       ccGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTaa                                                                                                             <  1:550717/140‑1 (MQ=255)                        cgcgCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTAAg                                                                                                            <  2:29204/140‑1 (MQ=255)                            cTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGa                                                                                                        <  2:476690/140‑1 (MQ=255)                               cACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGATTg                                                                                                     <  2:498546/140‑1 (MQ=255)                                         gCGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAg                                                                                           <  1:990974/140‑1 (MQ=255)                                          cGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAgc                                                                                          <  2:938862/140‑1 (MQ=255)                                          cGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAgc                                                                                          <  1:865806/140‑1 (MQ=255)                                              tACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCgg                                                                                      >  1:509965/1‑140 (MQ=255)                                                   accccTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCTCGGAAGCGCGGTAATa                                                                                 <  1:882591/137‑1 (MQ=255)                                                       ctctGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCtt                                                                             >  1:874642/1‑140 (MQ=255)                                                                gggAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCg                                                                    <  2:180848/140‑1 (MQ=255)                                                                gggAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCg                                                                    <  2:862370/140‑1 (MQ=255)                                                                  gAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCg                                                                    <  2:281234/138‑1 (MQ=255)                                                                  gAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCg                                                                    >  1:281234/1‑138 (MQ=255)                                                                           cTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGAtg                                                         <  2:874642/140‑1 (MQ=255)                                                                                          ctcgcgctACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTa                                          >  1:1564/1‑140 (MQ=255)                                                                                                       cATGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCaa                             >  2:77995/1‑140 (MQ=255)                                                                                                         tGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTcg                                    <  2:282053/131‑1 (MQ=255)                                                                                                         tGGAGCCAGAAGTCACAATGGCCCACGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTcg                                    >  1:282053/1‑131 (MQ=255)                                                                                                                              cccACGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGcc      >  1:567202/1‑140 (MQ=255)                                                                                                                                  cGGAATGCCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATaa  >  1:555423/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      ATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAA  >  NC_000913/2411658‑2411926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑