Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,572,295	A→G	C52C (TGT→TGC)	eutM ←	putative structural protein, ethanolamine utilization microcompartment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,572,295	0	A	G	100.0%	224.1 / NA	63	C52C (TGT→TGC)	eutM	putative structural protein, ethanolamine utilization microcompartment
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/34);  total (29/34)

ACAGGTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTT  >  NC_000913/2572159‑2572428                                                                                                                                         |                                                                                                                                      aCAGGTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAg                                                                                                                                    <  2:332172/140‑1 (MQ=255)      ttGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGc                                                                                                                               <  2:349339/140‑1 (MQ=255)        gctgctATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGTTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCaa                                                                                                                             >  2:376082/1‑140 (MQ=255)        gctgctATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCaa                                                                                                                             >  2:910262/1‑140 (MQ=255)        gctgctATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCaa                                                                                                                             >  2:105294/1‑140 (MQ=255)         ctgctATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAAc                                                                                                                            >  1:643244/1‑140 (MQ=255)               tCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTg                                                                                                                                <  2:601500/130‑1 (MQ=255)               tCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTg                                                                                                                                >  1:601500/1‑130 (MQ=255)                cGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATcacca                                                                                                                     <  2:919529/140‑1 (MQ=255)                cGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATcacca                                                                                                                     <  1:910262/140‑1 (MQ=255)                     ttCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAAc                                                                                                                <  2:116856/140‑1 (MQ=255)                       cAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCa                                                                                                              <  1:626812/140‑1 (MQ=255)                       cAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCa                                                                                                              <  2:196041/140‑1 (MQ=255)                       cAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCa                                                                                                              <  1:362681/140‑1 (MQ=255)                         ggCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATg                                                                                                            >  2:471426/1‑140 (MQ=255)                           ccGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTGCAGGCTGCAACATCACCACGAACCATGGc                                                                                                          <  2:49467/140‑1 (MQ=255)                             gATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAg                                                                                                        >  2:671287/1‑140 (MQ=255)                             gATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAg                                                                                                        >  1:669685/1‑140 (MQ=255)                               tCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTa                                                                                                      >  1:318518/1‑140 (MQ=255)                                        cttcttCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGgccg                                                                                             >  2:20200/1‑140 (MQ=255)                                           cttcCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGgc                                                                                               >  2:787408/1‑135 (MQ=255)                                           cttcCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGgc                                                                                               <  1:787408/135‑1 (MQ=255)                                              ccAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCa                                                                                       >  1:574735/1‑140 (MQ=255)                                              ccAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCa                                                                                       <  2:102486/140‑1 (MQ=255)                                                aGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAAt                                                                                     <  2:990951/140‑1 (MQ=255)                                                aGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAAt                                                                                     <  1:784423/140‑1 (MQ=255)                                                aGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAAt                                                                                     <  1:471426/140‑1 (MQ=255)                                                aGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAAt                                                                                     <  1:290779/140‑1 (MQ=255)                                                     gCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCt                                                                                >  2:43354/1‑140 (MQ=255)                                                      ccGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAAt                                                                                     >  1:813211/1‑134 (MQ=255)                                                      ccGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAAt                                                                                     <  2:813211/134‑1 (MQ=255)                                                                        aCGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGAc                                                             >  2:135166/1‑140 (MQ=255)                                                                             tACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGAcgcgcg                                                        <  1:619943/140‑1 (MQ=255)                                                                             tACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCACCAATCTGCTTAACACCAACCAGCTTGAcgcgcg                                                        <  2:172336/140‑1 (MQ=255)                                                                              aCAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGAcgcgcg                                                        <  1:144761/139‑1 (MQ=255)                                                                              aCAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGAcgcgcg                                                        >  2:144761/1‑139 (MQ=255)                                                                               cAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCa                                                      <  2:669685/140‑1 (MQ=255)                                                                                  aTACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGAcgcgcg                                                        >  1:906118/1‑135 (MQ=255)                                                                                  aTACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGAcgcgcg                                                        <  2:906118/135‑1 (MQ=255)                                                                                  aTACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCt                                                   >  1:423709/1‑140 (MQ=255)                                                                                    aCCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCttt                                                 >  2:92844/1‑140 (MQ=255)                                                                                    aCCAGCTCGCCGATACGCTGTGCCACTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCttt                                                 >  1:756462/1‑140 (MQ=255)                                                                                     ccAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTc                                                <  1:800421/140‑1 (MQ=255)                                                                                       aGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCAc                                              >  2:489003/1‑140 (MQ=255)                                                                                       aGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCAc                                              <  2:318518/140‑1 (MQ=255)                                                                                            gCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGAcgcgcg                                                        <  1:29092/125‑1 (MQ=255)                                                                                            gCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGAcgcgcg                                                        >  2:29092/1‑125 (MQ=255)                                                                                            gCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATcg                                         >  1:421651/1‑140 (MQ=255)                                                                                                 tACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCa                                    >  1:324883/1‑140 (MQ=255)                                                                                                      tgtgCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGc                               <  2:324883/140‑1 (MQ=255)                                                                                                        tgCCGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCt                             >  2:707684/1‑140 (MQ=255)                                                                                                          ccGCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCa                           <  1:671287/140‑1 (MQ=255)                                                                                                            gCTGCGGCCCCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAAt                         <  2:423709/140‑1 (MQ=255)                                                                                                            gCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAAt                         <  2:574735/140‑1 (MQ=255)                                                                                                            gCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAAt                         >  2:757286/1‑140 (MQ=255)                                                                                                            gCTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAAt                         <  1:489003/140‑1 (MQ=255)                                                                                                             cTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATc                        <  2:643244/140‑1 (MQ=255)                                                                                                             cTGCGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATc                        >  1:780244/1‑140 (MQ=255)                                                                                                                cGGCACCTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATAGCGTCAGAAGCCTCAATCAGt                     <  1:92844/140‑1 (MQ=255)                                                                                                                     ccTGCATCGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCACGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAAc                >  2:992228/1‑140 (MQ=255)                                                                                                                            cGGTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGccc         <  1:135166/140‑1 (MQ=255)                                                                                                                             ggTCGCGGCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGcccc        <  1:105294/140‑1 (MQ=255)                                                                                                                                   ggCTTTGCAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGttt  >  2:481591/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      ACAGGTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTT  >  NC_000913/2572159‑2572428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑