Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,695,848	G→A	L16L (CTG→CTA)	mltF →	membrane‑bound lytic murein transglycosylase F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,695,848	0	G	A	100.0%	149.9 / NA	44	L16L (CTG→CTA)	mltF	membrane‑bound lytic murein transglycosylase F
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/21);  total (23/21)

AATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCA  >  NC_000913/2695718‑2695978                                                                                                                                   |                                                                                                                                   aaTGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGAACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTcgcg                                                                                                                           >  2:892545/1‑140 (MQ=255)                   gTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGccatcc                                                                                                         >  2:38261/1‑140 (MQ=255)                   gTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGccatcc                                                                                                         <  2:867508/140‑1 (MQ=255)                     aaaCCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGccatccat                                                                                                       >  2:815364/1‑140 (MQ=255)                       aCCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGccatcc                                                                                                         <  2:665956/136‑1 (MQ=255)                       aCCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGccatcc                                                                                                         >  1:665956/1‑136 (MQ=255)                            tCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGccatccat                                                                                                       <  2:293512/133‑1 (MQ=255)                            tCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGccatccat                                                                                                       >  1:293512/1‑133 (MQ=255)                            tCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTgg                                                                                                <  2:525573/140‑1 (MQ=255)                            tCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTgg                                                                                                >  1:727169/1‑140 (MQ=255)                                  ttattaCGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGccatcc                                                                                                         >  1:570932/1‑125 (MQ=255)                                  ttattaCGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGccatcc                                                                                                         <  2:570932/125‑1 (MQ=255)                                   tattaCGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTa                                                                                         <  2:727169/140‑1 (MQ=255)                                          ttttttCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTa                                                                                         >  1:73103/1‑133 (MQ=255)                                          ttttttCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTa                                                                                         <  2:73103/133‑1 (MQ=255)                                          ttttttCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGa                                                                                  >  1:637630/1‑140 (MQ=255)                                          ttttttCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGa                                                                                  >  1:277842/1‑140 (MQ=255)                                          ttttttCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGa                                                                                  <  1:9633/140‑1 (MQ=255)                                            ttttCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCg                                                                                   >  1:325075/1‑137 (MQ=255)                                            ttttCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCg                                                                                   <  2:325075/137‑1 (MQ=255)                                            ttttCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACa                                                                                >  1:52542/1‑140 (MQ=255)                                            ttttCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACa                                                                                >  1:811560/1‑140 (MQ=255)                                             tttCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACaa                                                                               >  2:193378/1‑140 (MQ=255)                                                      gggACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATcgc                                                                      <  1:892545/140‑1 (MQ=255)                                                       ggACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATcgcc                                                                     >  2:188517/1‑140 (MQ=255)                                                            cgcACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCAGACAACCGTATCGCCGCCAt                                                                >  2:474144/1‑140 (MQ=255)                                                                  acacAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAgc                                                          <  2:811560/140‑1 (MQ=255)                                                                  acacAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCAGACAACCGTATCGCCGCCATTCAAgc                                                          <  1:474144/140‑1 (MQ=255)                                                                         gaATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGga                                                   >  1:398559/1‑140 (MQ=255)                                                                          aaTTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGTCGACAACCGTATCGCCGCCATTCAAGCGCGGGgag                                                  <  1:227574/140‑1 (MQ=255)                                                                               aCTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTg                                             <  2:277842/140‑1 (MQ=255)                                                                                    ttGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCgtgt                                        <  1:38261/140‑1 (MQ=255)                                                                                    ttGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCgtgt                                        >  2:480925/1‑140 (MQ=255)                                                                                       aaaaaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAg                                     <  2:398559/140‑1 (MQ=255)                                                                                             ttAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCTGGGAGAGTTGCGTGTGAGCACCAt                               <  1:193378/140‑1 (MQ=255)                                                                                             ttAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCAt                               <  1:815364/140‑1 (MQ=255)                                                                                                        ttaTCTGTTCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTcccc                    <  2:127996/140‑1 (MQ=255)                                                                                                               ttCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTcccc                    <  1:780542/133‑1 (MQ=255)                                                                                                               ttCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTcccc                    >  2:780542/1‑133 (MQ=255)                                                                                                               ttCATCGGCATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTta             >  2:35097/1‑140 (MQ=255)                                                                                                                       cATTCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCACTGACTTATAACGaaa     >  1:254222/1‑140 (MQ=255)                                                                                                                         ttCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATc   >  1:193824/1‑140 (MQ=255)                                                                                                                         ttCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCACTGACTTATAACGAAATc   <  2:254222/140‑1 (MQ=255)                                                                                                                          tCTGGCACTACTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCa  >  2:158785/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                   AATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCA  >  NC_000913/2695718‑2695978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑