Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	447,883	A→G	V55A (GTG→GCG)	cyoD ←	cytochrome bo3 ubiquinol oxidase subunit 4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	447,883	0	A	G	100.0%	302.5 / NA	84	V55A (GTG→GCG)	cyoD	cytochrome bo3 ubiquinol oxidase subunit 4
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/41);  total (43/41)

TCCACATAATCCAGATGGAGCCTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCACTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGACG  >  NC_000913/447746‑448018                                                                                                                                          |                                                                                                                                        tCCACATAATCCAGATGGAGCCTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCt                                                                                                                                       >  1:69699/1‑140 (MQ=255)    acaTAATCCAGATGGAGCCTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGcc                                                                                                                                    <  1:438674/140‑1 (MQ=255)    acaTAATCCAGATGGAGCCTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGcc                                                                                                                                    >  1:49898/1‑140 (MQ=255)    acaTAATCCAGATGGAGCCTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGcc                                                                                                                                    <  2:594857/140‑1 (MQ=255)    acaTAATCCAGATGGAGCCTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGcc                                                                                                                                    >  1:594857/1‑140 (MQ=255)          tCCAGATGGAGCCTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGcc                                                                                                                              >  2:109752/1‑140 (MQ=255)              gATGGAGCCTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGa                                                                                                                          <  1:342158/140‑1 (MQ=255)               aTGGAGCCTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGAt                                                                                                                         >  2:537891/1‑140 (MQ=255)                tGGAGCCTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGAtt                                                                                                                        >  1:476864/1‑140 (MQ=255)                    gCCTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGc                                                                                                                               <  1:116796/129‑1 (MQ=255)                    gCCTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGc                                                                                                                               >  2:116796/1‑129 (MQ=255)                    gCCTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGAt                                                                                                                         >  2:39753/1‑135 (MQ=255)                    gCCTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGAt                                                                                                                         <  1:39753/135‑1 (MQ=255)                    gCCTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTc                                                                                                                    >  1:690284/1‑140 (MQ=255)                     ccTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTcc                                                                                                                   <  2:272032/140‑1 (MQ=255)                     ccTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTcc                                                                                                                   >  2:241777/1‑140 (MQ=255)                     ccTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTcc                                                                                                                   <  2:138764/140‑1 (MQ=255)                         cAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGAtt                                                                                                                        >  2:241789/1‑131 (MQ=255)                         cAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGAtt                                                                                                                        <  1:241789/131‑1 (MQ=255)                                   aGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCg                                                                                                     <  2:69699/140‑1 (MQ=255)                                   aGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCg                                                                                                     >  2:383197/1‑140 (MQ=255)                                   aGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCg                                                                                                     >  2:734101/1‑140 (MQ=255)                                    gCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCgg                                                                                                    >  2:16149/1‑140 (MQ=255)                                    gCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCgg                                                                                                    >  1:777737/1‑140 (MQ=255)                                     cGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGATGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGga                                                                                                   <  2:906983/140‑1 (MQ=255)                                     cGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGga                                                                                                   <  2:777737/140‑1 (MQ=255)                                     cGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGga                                                                                                   <  1:537891/140‑1 (MQ=255)                                     cGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGga                                                                                                   >  2:330129/1‑140 (MQ=255)                                     cGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGga                                                                                                   <  1:77203/140‑1 (MQ=255)                                       atgatTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGgaga                                                                                                 >  2:951296/1‑140 (MQ=255)                                       atgatTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGga                                                                                                   <  1:596064/138‑1 (MQ=255)                                       atgatTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGga                                                                                                   >  2:596064/1‑138 (MQ=255)                                            tAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAg                                                                                            <  1:734101/140‑1 (MQ=255)                                              gCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCCGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCt                                                                                          <  2:690284/140‑1 (MQ=255)                                              gCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCt                                                                                          <  2:49898/140‑1 (MQ=255)                                              gCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCt                                                                                          <  1:104900/140‑1 (MQ=255)                                              gCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCt                                                                                          <  1:352875/140‑1 (MQ=255)                                                   gTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGt                                                                                     <  2:856375/140‑1 (MQ=255)                                                    tGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGtc                                                                                    >  2:322372/1‑140 (MQ=255)                                                    tGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGtc                                                                                    >  1:887889/1‑140 (MQ=255)                                                    tGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGtc                                                                                    >  1:534366/1‑140 (MQ=255)                                                     gAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGtca                                                                                   <  1:911302/140‑1 (MQ=255)                                                        gACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGtcatca                                                                                <  2:187123/140‑1 (MQ=255)                                                         aCAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCAc                                                                               >  2:330501/1‑140 (MQ=255)                                                             aCGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCt                                                                                          <  1:419090/125‑1 (MQ=255)                                                             aCGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCt                                                                                          >  2:419090/1‑125 (MQ=255)                                                             aCGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCAGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATc                                                                           >  2:809584/1‑140 (MQ=255)                                                              cGCCGTCATGTTCCAGCCTTCCTCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATcc                                                                          >  2:708766/1‑140 (MQ=255)                                                              cGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATcc                                                                          >  1:193141/1‑140 (MQ=255)                                                                ccGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGTAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCTTGTCATCCCCATCCAg                                                                        <  2:212871/140‑1 (MQ=255)                                                                ccGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCt                                                                                          <  1:964858/122‑1 (MQ=255)                                                                ccGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCt                                                                                          >  2:964858/1‑122 (MQ=255)                                                                       gTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGaa                                                                 >  1:880156/1‑140 (MQ=255)                                                                       gTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGaa                                                                 >  1:227679/1‑140 (MQ=255)                                                                            aGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCAcc                                                            <  1:330129/140‑1 (MQ=255)                                                                             gCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCg                                                           >  1:554690/1‑140 (MQ=255)                                                                             gCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCg                                                           >  1:358622/1‑140 (MQ=255)                                                                             gCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCg                                                           >  1:657135/1‑140 (MQ=255)                                                                              ccTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGt                                                          <  1:109752/140‑1 (MQ=255)                                                                              ccTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGt                                                          <  1:322372/140‑1 (MQ=255)                                                                                  cATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGa                                                      >  2:941848/1‑140 (MQ=255)                                                                                          ttGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGa                                              <  1:392945/140‑1 (MQ=255)                                                                                            ggTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACa                                            >  1:59703/1‑140 (MQ=255)                                                                                                ttCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGAt                                        <  2:534366/140‑1 (MQ=255)                                                                                                ttCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGAt                                        <  1:807446/140‑1 (MQ=255)                                                                                                   aTGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATaaa                                     >  2:368723/1‑140 (MQ=255)                                                                                                       gCAGGAAGCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCt                                 >  2:218473/1‑140 (MQ=255)                                                                                                              gCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGt                          <  2:227679/140‑1 (MQ=255)                                                                                                              gCACACCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGt                          <  2:880156/140‑1 (MQ=255)                                                                                                               cacaCCAGATGCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTa                         >  2:688530/1‑140 (MQ=255)                                                                                                                         gCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATGCAGAACGGAGTCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACg               >  2:893076/1‑140 (MQ=255)                                                                                                                         gCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACg               <  1:368723/140‑1 (MQ=255)                                                                                                                         gCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACg               <  2:193141/140‑1 (MQ=255)                                                                                                                         gCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACg               <  1:24530/140‑1 (MQ=255)                                                                                                                         gCACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACg               <  1:218473/140‑1 (MQ=255)                                                                                                                         gCACCAGAACCAGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACg               <  1:809584/140‑1 (MQ=255)                                                                                                                          cACCAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGc              <  1:708766/140‑1 (MQ=255)                                                                                                                            ccAGAACCTGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTg            >  1:200766/1‑140 (MQ=255)                                                                                                                                 aCCTGTACCGCTGCCATTGCCAGGATTGTTCACAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATg       >  2:978173/1‑140 (MQ=255)                                                                                                                                   cTGTACCGCTGCCATTGCCAGGATTCTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATggg     <  2:887889/140‑1 (MQ=255)                                                                                                                                    tGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACg               <  1:211213/129‑1 (MQ=255)                                                                                                                                    tGTACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACg               >  2:211213/1‑129 (MQ=255)                                                                                                                                      tACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGAcg  >  1:436376/1‑140 (MQ=255)                                                                                                                                      tACCGCTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGAcg  >  2:446509/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        TCCACATAATCCAGATGGAGCCTACAACCAGGATAGCGATGATTAGCACGGTGAAGACAAACGCCGTCATGTTCCAGCCTTCATCTGATTTGGTATTCATGTGCAGGAAGCACACCAGATGCACCAGAACCTGTACCACTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGACG  >  NC_000913/447746‑448018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑